FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2588294589> ?p ?o ?g. }

• W2588294589 endingPage "1103" @default.
• W2588294589 startingPage "1095" @default.
• W2588294589 abstract "Polysaccharide monooxygenases (PMOs) are secreted metalloenzymes that catalyze the oxidative degradation of polysaccharides in a copper-, oxygen-, and reductant-dependent manner. Cellulose-active fungal PMOs degrade cellulosic substrates to be utilized as a carbon source for fungal growth. To gain insight into the PMO mechanism, the role of conserved residues in the copper coordination sphere was investigated. Here, we report active-site hydrogen-bonding motifs in the secondary copper coordination sphere of MtPMO3*, a C1-oxidizing PMO from the ascomycete fungus Myceliophthora thermophila. A series of point substitutions that disrupt this conserved network are used to interrogate its function. Activity assays, in conjunction with EPR spectroscopy, demonstrate that residues H161 and Q167 are involved in stabilizing bound oxygen, and H161 appears to play a role in proton transfer. Additionally, Q167 increases the ligand donor strength of Y169 to the copper via a hydrogen-bonding interaction. Altogether, H161 and Q167 are important for oxygen activation, and the results are suggestive of a copper-oxyl active intermediate." @default.
• W2588294589 created "2017-02-24" @default.
• W2588294589 creator A5031634252 @default.
• W2588294589 creator A5033319200 @default.
• W2588294589 creator A5039561796 @default.
• W2588294589 creator A5072187299 @default.
• W2588294589 creator A5086254191 @default.
• W2588294589 date "2017-03-03" @default.
• W2588294589 modified "2023-10-13" @default.
• W2588294589 title "The Role of the Secondary Coordination Sphere in a Fungal Polysaccharide Monooxygenase" @default.
• W2588294589 cites W1539796472 @default.
• W2588294589 cites W1722029454 @default.
• W2588294589 cites W1876058484 @default.
• W2588294589 cites W1966889444 @default.
• W2588294589 cites W1967796434 @default.
• W2588294589 cites W1970713347 @default.
• W2588294589 cites W1975165348 @default.
• W2588294589 cites W1995038206 @default.
• W2588294589 cites W1996442036 @default.
• W2588294589 cites W1997124914 @default.
• W2588294589 cites W2000291296 @default.
• W2588294589 cites W2001641653 @default.
• W2588294589 cites W2011773599 @default.
• W2588294589 cites W2022734245 @default.
• W2588294589 cites W2023071558 @default.
• W2588294589 cites W2036635330 @default.
• W2588294589 cites W2048645097 @default.
• W2588294589 cites W2050782835 @default.
• W2588294589 cites W2056453014 @default.
• W2588294589 cites W2058713295 @default.
• W2588294589 cites W2063796154 @default.
• W2588294589 cites W2063908784 @default.
• W2588294589 cites W2069097530 @default.
• W2588294589 cites W2077621515 @default.
• W2588294589 cites W2091513159 @default.
• W2588294589 cites W2091605104 @default.
• W2588294589 cites W2092383076 @default.
• W2588294589 cites W2108274584 @default.
• W2588294589 cites W2113975655 @default.
• W2588294589 cites W2129774902 @default.
• W2588294589 cites W2144081223 @default.
• W2588294589 cites W2146051607 @default.
• W2588294589 cites W2152513829 @default.
• W2588294589 cites W2168506412 @default.
• W2588294589 cites W2169442257 @default.
• W2588294589 cites W2179113683 @default.
• W2588294589 cites W2291103326 @default.
• W2588294589 cites W2328433590 @default.
• W2588294589 cites W2561236934 @default.
• W2588294589 cites W2593815967 @default.
• W2588294589 doi "https://doi.org/10.1021/acschembio.7b00016" @default.
• W2588294589 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5554615" @default.
• W2588294589 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28257189" @default.
• W2588294589 hasPublicationYear "2017" @default.
• W2588294589 type Work @default.
• W2588294589 sameAs 2588294589 @default.
• W2588294589 citedByCount "85" @default.
• W2588294589 countsByYear W25882945892017 @default.
• W2588294589 countsByYear W25882945892018 @default.
• W2588294589 countsByYear W25882945892019 @default.
• W2588294589 countsByYear W25882945892020 @default.
• W2588294589 countsByYear W25882945892021 @default.
• W2588294589 countsByYear W25882945892022 @default.
• W2588294589 countsByYear W25882945892023 @default.
• W2588294589 crossrefType "journal-article" @default.
• W2588294589 hasAuthorship W2588294589A5031634252 @default.
• W2588294589 hasAuthorship W2588294589A5033319200 @default.
• W2588294589 hasAuthorship W2588294589A5039561796 @default.
• W2588294589 hasAuthorship W2588294589A5072187299 @default.
• W2588294589 hasAuthorship W2588294589A5086254191 @default.
• W2588294589 hasBestOaLocation W25882945892 @default.
• W2588294589 hasConcept C100817775 @default.
• W2588294589 hasConcept C11111787 @default.
• W2588294589 hasConcept C112887158 @default.
• W2588294589 hasConcept C116569031 @default.
• W2588294589 hasConcept C13002179 @default.
• W2588294589 hasConcept C170493617 @default.
• W2588294589 hasConcept C178790620 @default.
• W2588294589 hasConcept C181199279 @default.
• W2588294589 hasConcept C185592680 @default.
• W2588294589 hasConcept C2779251873 @default.
• W2588294589 hasConcept C32909587 @default.
• W2588294589 hasConcept C41183919 @default.
• W2588294589 hasConcept C526171541 @default.
• W2588294589 hasConcept C544778455 @default.
• W2588294589 hasConcept C55493867 @default.
• W2588294589 hasConcept C71240020 @default.
• W2588294589 hasConceptScore W2588294589C100817775 @default.
• W2588294589 hasConceptScore W2588294589C11111787 @default.
• W2588294589 hasConceptScore W2588294589C112887158 @default.
• W2588294589 hasConceptScore W2588294589C116569031 @default.
• W2588294589 hasConceptScore W2588294589C13002179 @default.
• W2588294589 hasConceptScore W2588294589C170493617 @default.
• W2588294589 hasConceptScore W2588294589C178790620 @default.
• W2588294589 hasConceptScore W2588294589C181199279 @default.
• W2588294589 hasConceptScore W2588294589C185592680 @default.
• W2588294589 hasConceptScore W2588294589C2779251873 @default.
• W2588294589 hasConceptScore W2588294589C32909587 @default.

• next