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- W2588332046 abstract "Pepper is one of the most important crops in Korean agricultural industries. However, it has been caused by several pepper diseases, which damage the quality and reduce the production, including Phytophthora root rot (P. capsici), anthracnose (Colletotrichum acutatum and C. capsici), powdery mildew (Leveillula taurica), Tobacco mosaic virus (TMV), Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), and some Potyvirus. Therefore, it is getting important to develop pepper varieties which are resistant to these diseases and have high capsaicinoid content. For this reason, it is necessary to develop a high-throughput genotyping system which can analyze a number of samples with multiple SNP markers. In this study, we aimed to genotype 191 pepper samples with 24 SNP markers using Fluidigm 192.24 Dynamic Array, which yields 4,608 data points at one time. We re-designed 24 SNP markers from STS or HRM markers for Phytophthora root rot (M3-2, M3-3), anthracnose (CcR9, CA09g12180, CA09g19170), powdery mildew (Ltr4.1-40344, Ltr4.2-585119), CMV (Cmr1-2), TSWV (Tsw1-1, Tsw1-2), TMV (L4), Potyvirus (pvr1, pvr2), and capsaicinoid content (qdhc2.1-1335057, qdhc2.2-43829, qcap3.1-40134, qcap6.1-299931, qcap6.1-299931). Plant materials were a total of 191 samples consisting of 33 pepper cultivars, 23 paprika cultivars, 39 pepper accessions, and one negative control. As the result, 18 SNP markers were successfully analyzed. This approach can be helpful to multiplex many SNP markers for foreground selection in pepper breeding programs." @default.
- W2588332046 created "2017-02-24" @default.
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- W2588332046 date "2016-06-29" @default.
- W2588332046 modified "2023-09-27" @default.
- W2588332046 title "Application of SNP Markers for Disease Resistance and Capsaicinoid Content in Pepper and Paprika Cultivars Using Fluidigm EP1TM System" @default.
- W2588332046 hasPublicationYear "2016" @default.
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