FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2588352913> ?p ?o ?g. }

• W2588352913 abstract "Abstract Aim To establish a simplified and efficient protocol for the isolation and concentration of matrix proteins from human dentine, and to assess the effects of extracted dentine matrix proteins ( eDMP ) on the behaviour of human pulp cells. Methodology Matrix proteins were isolated from human dentine, purified, concentrated and characterized with protein and enzyme‐linked immunosorbent assays (ELISA). Culture media were supplemented with eDMP in different concentrations, referred to as eDMP 1–10 000, to assess viability and proliferation of human pulp cells by DNA and MTT assays; apoptotic events were quantified by flow cytometry. Chemotactic effects of eDMP were assessed in a modified Boyden chamber assay. Expression levels of odontoblastic marker genes in pulp cells cultured with eDMP s were determined by real‐time quantitative PCR, and the ability to induce mineralization was demonstrated by alizarin red staining. Nonparametric statistical analysis was performed to pairwise compare different groups at all time‐points (Mann–Whitney U ‐test, α = 0.05). Results High concentrations of eDMP exhibited significant antiproliferative effects ( P ≤ 0.023) after 5 ( eDMP 1000) and 7 days ( eDMP 500) without affecting cell viability. Apoptosis was barely influenced ( P ≥ 0.089). eDMP exerted a concentration‐dependent chemotactic stimulus on dental pulp cells with statistical significance already at low dosage ( P = 0.006 at eDMP 10). Changes in gene expression indicated a differentiation into odontoblast‐like cells, which was corroborated by findings of mineral nodule formation. Conclusions A novel, effective and time‐saving protocol for isolation and concentration of dentine matrix proteins is presented. As eDMP stimulates chemotaxis, differentiation and mineralization without affecting viability, endogenous dentine matrix proteins might be valuable for approaches to regenerate or engineer dental pulp." @default.
• W2588352913 created "2017-02-24" @default.
• W2588352913 creator A5003816815 @default.
• W2588352913 creator A5014229409 @default.
• W2588352913 creator A5032830434 @default.
• W2588352913 creator A5040546347 @default.
• W2588352913 creator A5041476458 @default.
• W2588352913 creator A5059385052 @default.
• W2588352913 creator A5072707127 @default.
• W2588352913 date "2017-03-14" @default.
• W2588352913 modified "2023-10-12" @default.
• W2588352913 title "Dentine matrix proteins: isolation and effects on human pulp cells" @default.
• W2588352913 cites W1636022999 @default.
• W2588352913 cites W1970457474 @default.
• W2588352913 cites W1978622017 @default.
• W2588352913 cites W1989880616 @default.
• W2588352913 cites W1991614762 @default.
• W2588352913 cites W1997812904 @default.
• W2588352913 cites W2008161915 @default.
• W2588352913 cites W2017011504 @default.
• W2588352913 cites W2019777614 @default.
• W2588352913 cites W2022838892 @default.
• W2588352913 cites W2023153539 @default.
• W2588352913 cites W2029365671 @default.
• W2588352913 cites W2035817026 @default.
• W2588352913 cites W2041809728 @default.
• W2588352913 cites W2055416616 @default.
• W2588352913 cites W2056023417 @default.
• W2588352913 cites W2059773838 @default.
• W2588352913 cites W2063034762 @default.
• W2588352913 cites W2072148716 @default.
• W2588352913 cites W2073322026 @default.
• W2588352913 cites W2075356405 @default.
• W2588352913 cites W2094197406 @default.
• W2588352913 cites W2102236548 @default.
• W2588352913 cites W2103554755 @default.
• W2588352913 cites W2109382896 @default.
• W2588352913 cites W2113597392 @default.
• W2588352913 cites W2114429558 @default.
• W2588352913 cites W2114570899 @default.
• W2588352913 cites W2116502042 @default.
• W2588352913 cites W2124912625 @default.
• W2588352913 cites W2131036394 @default.
• W2588352913 cites W2137986174 @default.
• W2588352913 cites W2139055053 @default.
• W2588352913 cites W2149767015 @default.
• W2588352913 cites W2159161003 @default.
• W2588352913 cites W2161829550 @default.
• W2588352913 cites W2203217203 @default.
• W2588352913 cites W2213204092 @default.
• W2588352913 cites W2315292709 @default.
• W2588352913 cites W2415427012 @default.
• W2588352913 cites W2419107273 @default.
• W2588352913 doi "https://doi.org/10.1111/iej.12754" @default.
• W2588352913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28211068" @default.
• W2588352913 hasPublicationYear "2017" @default.
• W2588352913 type Work @default.
• W2588352913 sameAs 2588352913 @default.
• W2588352913 citedByCount "34" @default.
• W2588352913 countsByYear W25883529132017 @default.
• W2588352913 countsByYear W25883529132018 @default.
• W2588352913 countsByYear W25883529132019 @default.
• W2588352913 countsByYear W25883529132020 @default.
• W2588352913 countsByYear W25883529132021 @default.
• W2588352913 countsByYear W25883529132022 @default.
• W2588352913 countsByYear W25883529132023 @default.
• W2588352913 crossrefType "journal-article" @default.
• W2588352913 hasAuthorship W2588352913A5003816815 @default.
• W2588352913 hasAuthorship W2588352913A5014229409 @default.
• W2588352913 hasAuthorship W2588352913A5032830434 @default.
• W2588352913 hasAuthorship W2588352913A5040546347 @default.
• W2588352913 hasAuthorship W2588352913A5041476458 @default.
• W2588352913 hasAuthorship W2588352913A5059385052 @default.
• W2588352913 hasAuthorship W2588352913A5072707127 @default.
• W2588352913 hasBestOaLocation W25883529131 @default.
• W2588352913 hasConcept C106487976 @default.
• W2588352913 hasConcept C153911025 @default.
• W2588352913 hasConcept C185592680 @default.
• W2588352913 hasConcept C188442228 @default.
• W2588352913 hasConcept C190283241 @default.
• W2588352913 hasConcept C199343813 @default.
• W2588352913 hasConcept C2520015 @default.
• W2588352913 hasConcept C2780783641 @default.
• W2588352913 hasConcept C43617362 @default.
• W2588352913 hasConcept C53227056 @default.
• W2588352913 hasConcept C553184892 @default.
• W2588352913 hasConcept C55493867 @default.
• W2588352913 hasConcept C71924100 @default.
• W2588352913 hasConcept C86803240 @default.
• W2588352913 hasConcept C95444343 @default.
• W2588352913 hasConceptScore W2588352913C106487976 @default.
• W2588352913 hasConceptScore W2588352913C153911025 @default.
• W2588352913 hasConceptScore W2588352913C185592680 @default.
• W2588352913 hasConceptScore W2588352913C188442228 @default.
• W2588352913 hasConceptScore W2588352913C190283241 @default.
• W2588352913 hasConceptScore W2588352913C199343813 @default.
• W2588352913 hasConceptScore W2588352913C2520015 @default.
• W2588352913 hasConceptScore W2588352913C2780783641 @default.
• W2588352913 hasConceptScore W2588352913C43617362 @default.
• W2588352913 hasConceptScore W2588352913C53227056 @default.

• next