FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2588432841> ?p ?o ?g. }

• W2588432841 abstract "Abstract Abstract 4946 Establishing the diagnosis of Myelodysplastic Syndromes (MDS) is a challenging task for hematologists due to the heterogeneity of this clinical entity. Several attempts have been made to include findings from advanced technologies to the diagnostic criteria of MDS, but still in the majority of cases, morphology of peripheral blood and bone marrow remains the cornerstone for the diagnosis. Flow cytometry(FC) can identify abnormal antigen expression on myeloid cells. FC has been proposed as a complementary method in the diagnosis of low and intermediate risk MDS, particularly for patients not exhibiting characteristic karyotype abnormalities. On the other hand, recent literature suggests that these findings are not MDS-related, questioning the specificity of immunophenotyping for the diagnosis of MDS. The aim of the present study is to maximize the utility of FC data and simplify their interpretation for the diagnosis of MDS, by developing new analytical approaches of digital data, other than the conventional sequential biparametric analysis. The applied methodology was based on a mathematical model of scale analysis. Bone marrow(BM) samples from 50 subjects were analysed for the expression of CD45PC7, CD11bPC5, CD16FITC and CD13PE (antigens by Beckman Coulter, FC500 flow cytometer Beckman Coulter). 36 patients were diagnosed with MDS (23 low risk, 13 high risk) and 14 patients had other than an MDS diagnosis (ITP, chronic idiopathic neutropenia, systemic lupus erythematosus, LGL leukemia, age-related cytopenias, aplastic anemia, myelofibrosis etc). Additionally, 3 BM samples of patients with post-MDS acute myeloid leukemia(AML) were analysed. The data used for the development of the mathematical model were the following: two populations (neutro1, neutron2) were gated according to their CD45 and CD13/CD16 antigen expression (Figure 1i-1v).Seven subpopulations of Neutrophils were defined on CD11b/CD16 density plot N=g+h+i and O=k+j (Figure 1vi). In an attempt to identify correlations between data that cannot be routinely revealed by sequential biparametric analysis, we have developed the HeSK* ratio, which is given by: where x is the median of CD11b in gate O, y is the median of CD16 in gate O, z is the median of CD45 in gate neutro, pO is the percentage of gate O in the total CD11b/CD16 diagram gated in neutro, pN is the percentage of gate N in the total CD11b/CD16 gated in neutro and 1000 is an empirical parameter. The HeSK ratio combines fluorescence levels of CD16, CD11b and CD45 with the percentage of two distinct neutrophil populations (N and O), which differ in their maturation and differentiation stage. The ratio can quantify the abnormal differentiation profile of mature myeloid cells and thus distinguish MDS from non-MDS samples with statistical significance P<0. 0001 (Kruskal Wallis test) as indicated in graph 1. Descriptive statistics are shown in table 1. · HeSK ratio is based upon a novel FC analysis method that could change the conventional biparametric routine FC analysis and quantify patterns that are not evaluated properly. Mathematical modeling of antigen expression patterns optimizes the interpretation of single immunophenotype findings. · The present study proposes HeSK as a complementary diagnostic tool for MDS and a strong indicator for the classification of the patients according to their prognosis as well. *the name HeSK comes from the initials of the 4 main authors (H=Hala, e=Evgenia, S=Smirni, K=Kolliopoulou). Table 1 non MDS low risk MDS high risk MDS Number of values 14 23 13 Minimum 50,76 4,789 0,2850 25% Percentile 304,8 26,11 17,05 Median 2133 92,52 47,64 75% Percentile 10650 228,9 144,3 Maximum 55040 3043 671,7 Mean 10320 316,1 122,7 Std. Deviation 17860 647,9 185,1 Std. Error 4773 135,1 51,33 Figure 1 Figure 1. Disclosures: No relevant conflicts of interest to declare." @default.
• W2588432841 created "2017-02-24" @default.
• W2588432841 creator A5007079508 @default.
• W2588432841 creator A5018731677 @default.
• W2588432841 creator A5024960041 @default.
• W2588432841 creator A5036037491 @default.
• W2588432841 creator A5037061424 @default.
• W2588432841 creator A5046315339 @default.
• W2588432841 creator A5059435805 @default.
• W2588432841 creator A5065368922 @default.
• W2588432841 creator A5065916355 @default.
• W2588432841 creator A5073818680 @default.
• W2588432841 creator A5075332628 @default.
• W2588432841 creator A5088869439 @default.
• W2588432841 creator A5091691045 @default.
• W2588432841 date "2012-11-16" @default.
• W2588432841 modified "2023-09-27" @default.
• W2588432841 title "Hesk Ratio: A Complementary Tool for the Diagnosis of Myelodysplastic Syndromes and a Novel Method for Flow Cytometry Analysis" @default.
• W2588432841 doi "https://doi.org/10.1182/blood.v120.21.4946.4946" @default.
• W2588432841 hasPublicationYear "2012" @default.
• W2588432841 type Work @default.
• W2588432841 sameAs 2588432841 @default.
• W2588432841 citedByCount "0" @default.
• W2588432841 crossrefType "journal-article" @default.
• W2588432841 hasAuthorship W2588432841A5007079508 @default.
• W2588432841 hasAuthorship W2588432841A5018731677 @default.
• W2588432841 hasAuthorship W2588432841A5024960041 @default.
• W2588432841 hasAuthorship W2588432841A5036037491 @default.
• W2588432841 hasAuthorship W2588432841A5037061424 @default.
• W2588432841 hasAuthorship W2588432841A5046315339 @default.
• W2588432841 hasAuthorship W2588432841A5059435805 @default.
• W2588432841 hasAuthorship W2588432841A5065368922 @default.
• W2588432841 hasAuthorship W2588432841A5065916355 @default.
• W2588432841 hasAuthorship W2588432841A5073818680 @default.
• W2588432841 hasAuthorship W2588432841A5075332628 @default.
• W2588432841 hasAuthorship W2588432841A5088869439 @default.
• W2588432841 hasAuthorship W2588432841A5091691045 @default.
• W2588432841 hasConcept C126322002 @default.
• W2588432841 hasConcept C142724271 @default.
• W2588432841 hasConcept C143998085 @default.
• W2588432841 hasConcept C196166836 @default.
• W2588432841 hasConcept C203014093 @default.
• W2588432841 hasConcept C2777063308 @default.
• W2588432841 hasConcept C2778461978 @default.
• W2588432841 hasConcept C2778729363 @default.
• W2588432841 hasConcept C2779282312 @default.
• W2588432841 hasConcept C2780007613 @default.
• W2588432841 hasConcept C2780076729 @default.
• W2588432841 hasConcept C2780817109 @default.
• W2588432841 hasConcept C29730261 @default.
• W2588432841 hasConcept C553184892 @default.
• W2588432841 hasConcept C71924100 @default.
• W2588432841 hasConceptScore W2588432841C126322002 @default.
• W2588432841 hasConceptScore W2588432841C142724271 @default.
• W2588432841 hasConceptScore W2588432841C143998085 @default.
• W2588432841 hasConceptScore W2588432841C196166836 @default.
• W2588432841 hasConceptScore W2588432841C203014093 @default.
• W2588432841 hasConceptScore W2588432841C2777063308 @default.
• W2588432841 hasConceptScore W2588432841C2778461978 @default.
• W2588432841 hasConceptScore W2588432841C2778729363 @default.
• W2588432841 hasConceptScore W2588432841C2779282312 @default.
• W2588432841 hasConceptScore W2588432841C2780007613 @default.
• W2588432841 hasConceptScore W2588432841C2780076729 @default.
• W2588432841 hasConceptScore W2588432841C2780817109 @default.
• W2588432841 hasConceptScore W2588432841C29730261 @default.
• W2588432841 hasConceptScore W2588432841C553184892 @default.
• W2588432841 hasConceptScore W2588432841C71924100 @default.
• W2588432841 hasLocation W25884328411 @default.
• W2588432841 hasOpenAccess W2588432841 @default.
• W2588432841 hasPrimaryLocation W25884328411 @default.
• W2588432841 hasRelatedWork W122351395 @default.
• W2588432841 hasRelatedWork W1928182686 @default.
• W2588432841 hasRelatedWork W2020847543 @default.
• W2588432841 hasRelatedWork W2327694594 @default.
• W2588432841 hasRelatedWork W2382810426 @default.
• W2588432841 hasRelatedWork W2485963307 @default.
• W2588432841 hasRelatedWork W2500778132 @default.
• W2588432841 hasRelatedWork W2549173985 @default.
• W2588432841 hasRelatedWork W2549681019 @default.
• W2588432841 hasRelatedWork W2551029969 @default.
• W2588432841 hasRelatedWork W2557824125 @default.
• W2588432841 hasRelatedWork W2567923817 @default.
• W2588432841 hasRelatedWork W2580585049 @default.
• W2588432841 hasRelatedWork W2801378611 @default.
• W2588432841 hasRelatedWork W2897675944 @default.
• W2588432841 hasRelatedWork W2979540147 @default.
• W2588432841 hasRelatedWork W2980041280 @default.
• W2588432841 hasRelatedWork W3005051076 @default.
• W2588432841 hasRelatedWork W3029919508 @default.
• W2588432841 hasRelatedWork W97609973 @default.
• W2588432841 isParatext "false" @default.
• W2588432841 isRetracted "false" @default.
• W2588432841 magId "2588432841" @default.
• W2588432841 workType "article" @default.