FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2588850413> ?p ?o ?g. }

• W2588850413 abstract "Abstract Background: Sickle cell disease (SCD) patients are frequently transfused and, finding adequate and safe blood component for them has a great impact in transfusion services and in patients clinical follow-up. DNA genotyping for RBC antigens is a tool for the management of these patients to overcome the limitations of hemagglutination assays. However, the polymerase chain reaction (PCR) by restriction fragment length polymorphism (RFLP) is time consuming. The aim of this study was to develop a rapid PCR specific sequence primer (SSP) approach for Duffy genotyping in SCD patients. STUDY DESIGN AND METHODS: Blood samples from 91 SCD patients were studied by hemagglutination and by PCR-SSP for Duffy system. All samples typed as Fy(b-) were submitted to adsorption and elution techniques to detect the weak expression of Fyb antigen. DNA was extracted by a commercial DNA extraction kit (QIAamp DNA, QIAGEN, Germany). Detection of FY*A, FY*B, FY*B-33, FY*B-265T and FY*B-298A were performed using specific primers by PCR-SSP. DNA amplification was carried out in a final volume of 20μL with identical reaction mixture and cycling conditions as follows: 0.2μg of genomic DNA, 0.2mM of each dNTP, 5% of glycerol, 1.5 units of platinum Taq polymerase (Invitrogen, Brazil) in the buffer supplied, 5.0mM of MgCl2 and 10.0 pmoles of each primer. PCR fragments were separated for 90 minutes at 100V using 0.5μg.mL-1 ethidium bromide-stained 2.0% agarose gel and visualized in a UV light apparatus (Eagle Eye II, EUA). Results: Table 1 shows the results of Duffy phenotyping, Duffy genotyping and gene frequencies in SCD patients The most common phenotype found in our study was Fy(a–b+), 35.2%. In three samples (3.3%) we detected weak Fyb antigen and identified FY*B-265T alelle. All samples phenotyped as Fy(a–b–) genotyped as (FYB-33/FYB-33). CONCLUSION: PCR-SSP is a method that contributes for easily and accurate Duffy genotyping for SCD patients. In our study, all SCD patients with Fy(a–b–) can be safely transfused with Fy(b+) red blood cell components with no risk for developing anti-Fyb saving rare Fy(a–b–) units. Table 1 Phenotype Genotype n Genotype Frequency Fy(a+b+) FYA/FYB 16 0.176 Fy(a–b+) FYB/FYB 23 0.253 FYB/FYB-265T 9 0.099 Fy(a+b–) FYA/FYA 18 0.198 FYA/FYB-33 4 0.044 Fy(a–b–) FYB-33/FYB-33 21 0.231 TOTAL 91 1.00" @default.
• W2588850413 created "2017-02-24" @default.
• W2588850413 creator A5002753016 @default.
• W2588850413 creator A5016216682 @default.
• W2588850413 creator A5041243009 @default.
• W2588850413 creator A5047861224 @default.
• W2588850413 creator A5056473514 @default.
• W2588850413 creator A5073859748 @default.
• W2588850413 date "2007-11-16" @default.
• W2588850413 modified "2023-09-27" @default.
• W2588850413 title "Rapid Duffy Genotyping in Sickle Cell Disease Patients Using PCR-SSP Is a Valuable Tool for Blood Component Selection." @default.
• W2588850413 doi "https://doi.org/10.1182/blood.v110.11.2901.2901" @default.
• W2588850413 hasPublicationYear "2007" @default.
• W2588850413 type Work @default.
• W2588850413 sameAs 2588850413 @default.
• W2588850413 citedByCount "0" @default.
• W2588850413 crossrefType "journal-article" @default.
• W2588850413 hasAuthorship W2588850413A5002753016 @default.
• W2588850413 hasAuthorship W2588850413A5016216682 @default.
• W2588850413 hasAuthorship W2588850413A5041243009 @default.
• W2588850413 hasAuthorship W2588850413A5047861224 @default.
• W2588850413 hasAuthorship W2588850413A5056473514 @default.
• W2588850413 hasAuthorship W2588850413A5073859748 @default.
• W2588850413 hasConcept C104317684 @default.
• W2588850413 hasConcept C135763542 @default.
• W2588850413 hasConcept C153911025 @default.
• W2588850413 hasConcept C159047783 @default.
• W2588850413 hasConcept C168007829 @default.
• W2588850413 hasConcept C17757408 @default.
• W2588850413 hasConcept C178790620 @default.
• W2588850413 hasConcept C185592680 @default.
• W2588850413 hasConcept C2777563447 @default.
• W2588850413 hasConcept C31467283 @default.
• W2588850413 hasConcept C49105822 @default.
• W2588850413 hasConcept C54355233 @default.
• W2588850413 hasConcept C552990157 @default.
• W2588850413 hasConcept C78063203 @default.
• W2588850413 hasConcept C86803240 @default.
• W2588850413 hasConceptScore W2588850413C104317684 @default.
• W2588850413 hasConceptScore W2588850413C135763542 @default.
• W2588850413 hasConceptScore W2588850413C153911025 @default.
• W2588850413 hasConceptScore W2588850413C159047783 @default.
• W2588850413 hasConceptScore W2588850413C168007829 @default.
• W2588850413 hasConceptScore W2588850413C17757408 @default.
• W2588850413 hasConceptScore W2588850413C178790620 @default.
• W2588850413 hasConceptScore W2588850413C185592680 @default.
• W2588850413 hasConceptScore W2588850413C2777563447 @default.
• W2588850413 hasConceptScore W2588850413C31467283 @default.
• W2588850413 hasConceptScore W2588850413C49105822 @default.
• W2588850413 hasConceptScore W2588850413C54355233 @default.
• W2588850413 hasConceptScore W2588850413C552990157 @default.
• W2588850413 hasConceptScore W2588850413C78063203 @default.
• W2588850413 hasConceptScore W2588850413C86803240 @default.
• W2588850413 hasLocation W25888504131 @default.
• W2588850413 hasOpenAccess W2588850413 @default.
• W2588850413 hasPrimaryLocation W25888504131 @default.
• W2588850413 hasRelatedWork W17396297 @default.
• W2588850413 hasRelatedWork W1982076953 @default.
• W2588850413 hasRelatedWork W2080534576 @default.
• W2588850413 hasRelatedWork W2153355221 @default.
• W2588850413 hasRelatedWork W2368927906 @default.
• W2588850413 hasRelatedWork W2376152126 @default.
• W2588850413 hasRelatedWork W2376690311 @default.
• W2588850413 hasRelatedWork W2605221517 @default.
• W2588850413 hasRelatedWork W2796139176 @default.
• W2588850413 hasRelatedWork W3179802576 @default.
• W2588850413 isParatext "false" @default.
• W2588850413 isRetracted "false" @default.
• W2588850413 magId "2588850413" @default.
• W2588850413 workType "article" @default.