Matches in SemOpenAlex for { <https://semopenalex.org/work/W2588973387> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2588973387 abstract "Germ cells originate from progenitor cells, denominated as primordial germ cells (PGC). PGC are of extragonadal origin and migrate toward the gonad primordia where they proliferate and, in gonochoristic species, differentiate into sperm cells in males and oocytes in females. At the time of sex differentiation gonads are prone to the influence of external factors, such as the water temperature. Elevation of temperature from 28oC to 36oC during the critical time of gonadal differentiation i.e. 10 days post fertilization until 20 days post fertilization (dpf) is known to bring about masculinization in genetically female Nile tilapia. The first study aimed at the characterization of the micro-morphology of temperature-treated but no masculinized genetic females. A temperature treatment leads to masculinization in 37% of genetic females. Macro-morphologically at 90 dpf in both, the treatment and the control group immature ovaries were found. Maturation commenced at 120 dpf. Micro-morphologically five oocyte stages were found; chromatin nucleolus phase, peri-nucleolus phase, cortical alveolus stage, vitellogenic stage and mature oocyte stage. Chromatin nucleolus phase and the peri-nucleolus phase, being the primary growth phases, were represented at all age groups, whereas the advanced stages surfaced later. The cortical alveolus stage developed at 120 dpf; the vitellogenic stage at 150 dpf; mature stage at 180 dpf. There were slight individual differences in oocyte development. The second study was conducted to identify and monitor the germline stem cells of Nile tilapia raised under different temperature regimes. They were identified with the aid of vasa and PCNA (Proliferating Cell Nuclear Antigen). Samples were sectioned after paraffin-embedding and Polly-freeze-tissue-freezing-medium-embedding. For producing sample sections the paraffin-embedding method was preferable. Ovarian germline stem cells of Nile tilapia were identified located at the germinal epithelium of ovigerous lamellae. Ovarian germline stem cells are occurring either singly or in clutches, and were found in both females that had not been masculinized by temperature treatment and untreated control females. The identification of ovarian germline stem cells was easy at a young age but, due to development of ovigerous lamella, it was tedious in mature ovaries. In summary, this study revealed the existence of germline stem cells and their location in non-masculinized temperature-treated females and control females using immunostaining with vasa and PCNA." @default.
- W2588973387 created "2017-02-24" @default.
- W2588973387 creator A5048653129 @default.
- W2588973387 date "2022-02-21" @default.
- W2588973387 modified "2023-09-26" @default.
- W2588973387 title "Development of ovarian germline stem cells in Nile tilapia (Oreochromis niloticus) reared under different temperature regimes" @default.
- W2588973387 doi "https://doi.org/10.53846/goediss-6132" @default.
- W2588973387 hasPublicationYear "2022" @default.
- W2588973387 type Work @default.
- W2588973387 sameAs 2588973387 @default.
- W2588973387 citedByCount "0" @default.
- W2588973387 crossrefType "dissertation" @default.
- W2588973387 hasAuthorship W2588973387A5048653129 @default.
- W2588973387 hasBestOaLocation W25889733871 @default.
- W2588973387 hasConcept C104317684 @default.
- W2588973387 hasConcept C105702510 @default.
- W2588973387 hasConcept C126322002 @default.
- W2588973387 hasConcept C134018914 @default.
- W2588973387 hasConcept C137487509 @default.
- W2588973387 hasConcept C16685009 @default.
- W2588973387 hasConcept C183873130 @default.
- W2588973387 hasConcept C190062978 @default.
- W2588973387 hasConcept C196843134 @default.
- W2588973387 hasConcept C2776690073 @default.
- W2588973387 hasConcept C2778658382 @default.
- W2588973387 hasConcept C2780129523 @default.
- W2588973387 hasConcept C2781471917 @default.
- W2588973387 hasConcept C28328180 @default.
- W2588973387 hasConcept C2909208804 @default.
- W2588973387 hasConcept C505870484 @default.
- W2588973387 hasConcept C54355233 @default.
- W2588973387 hasConcept C56366318 @default.
- W2588973387 hasConcept C63405245 @default.
- W2588973387 hasConcept C71924100 @default.
- W2588973387 hasConcept C86803240 @default.
- W2588973387 hasConcept C88972607 @default.
- W2588973387 hasConcept C95444343 @default.
- W2588973387 hasConceptScore W2588973387C104317684 @default.
- W2588973387 hasConceptScore W2588973387C105702510 @default.
- W2588973387 hasConceptScore W2588973387C126322002 @default.
- W2588973387 hasConceptScore W2588973387C134018914 @default.
- W2588973387 hasConceptScore W2588973387C137487509 @default.
- W2588973387 hasConceptScore W2588973387C16685009 @default.
- W2588973387 hasConceptScore W2588973387C183873130 @default.
- W2588973387 hasConceptScore W2588973387C190062978 @default.
- W2588973387 hasConceptScore W2588973387C196843134 @default.
- W2588973387 hasConceptScore W2588973387C2776690073 @default.
- W2588973387 hasConceptScore W2588973387C2778658382 @default.
- W2588973387 hasConceptScore W2588973387C2780129523 @default.
- W2588973387 hasConceptScore W2588973387C2781471917 @default.
- W2588973387 hasConceptScore W2588973387C28328180 @default.
- W2588973387 hasConceptScore W2588973387C2909208804 @default.
- W2588973387 hasConceptScore W2588973387C505870484 @default.
- W2588973387 hasConceptScore W2588973387C54355233 @default.
- W2588973387 hasConceptScore W2588973387C56366318 @default.
- W2588973387 hasConceptScore W2588973387C63405245 @default.
- W2588973387 hasConceptScore W2588973387C71924100 @default.
- W2588973387 hasConceptScore W2588973387C86803240 @default.
- W2588973387 hasConceptScore W2588973387C88972607 @default.
- W2588973387 hasConceptScore W2588973387C95444343 @default.
- W2588973387 hasLocation W25889733871 @default.
- W2588973387 hasOpenAccess W2588973387 @default.
- W2588973387 hasPrimaryLocation W25889733871 @default.
- W2588973387 hasRelatedWork W1145599266 @default.
- W2588973387 hasRelatedWork W1965952977 @default.
- W2588973387 hasRelatedWork W2007573057 @default.
- W2588973387 hasRelatedWork W2015750766 @default.
- W2588973387 hasRelatedWork W2071544603 @default.
- W2588973387 hasRelatedWork W2364139764 @default.
- W2588973387 hasRelatedWork W2385325865 @default.
- W2588973387 hasRelatedWork W2588973387 @default.
- W2588973387 hasRelatedWork W2903522182 @default.
- W2588973387 hasRelatedWork W2945581648 @default.
- W2588973387 isParatext "false" @default.
- W2588973387 isRetracted "false" @default.
- W2588973387 magId "2588973387" @default.
- W2588973387 workType "dissertation" @default.