Matches in SemOpenAlex for { <https://semopenalex.org/work/W2589554599> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2589554599 endingPage "e13086" @default.
- W2589554599 startingPage "e13086" @default.
- W2589554599 abstract "e13086 Background: Gene therapy is a promising therapeutic approach for cancer. Targeted expression of desired therapeutic proteins within the tumor is the best approach to reduce toxicity and improve survival. This study was to establish a more effective and less toxic gene therapy of cancer. Methods: Combined gene therapy strategy with recombinant adenovirus expressing horseradish peroxidase (HRP) mediated by human telomerase reverse transcriptase (hTERT) promoter (AdhTERTHRP) and murine interleukin-12 (mIL-12) under the control of Cytomegalovirus (CMV) promoter (AdCMVmIL-12) was developed and evaluated against Lewis lung carcinoma (LLC) both in vivo and in vitro. The mechanism of action and systemic toxicities were also investigated. Results: HRP and mIL-12 proteins were detected in LLC cells and subcutaneous tumors. The combination of AdhTERTHRP/IAA treatment and AdCMVmIL-12 resulted in significant tumor growth inhibition and survival improvement compared with AdhTERTHRP/IAA alone (mean tumor volume, 427.4 ± 48.7 mm3 vs 581.9 ± 46.9 mm3, p = 0.005 on day 15; median overall survival (OS), 51 d vs 33 d) or AdCMVmIL-12 alone (mean tumor volume, 362.2 ± 33.8 mm3 vs 494.4 ± 70.2 mm3, p = 0.046 on day 12; median OS, 51 d vs 36 d). The expressed mIL-12 in tumor in combination group stimulated more CD4+ T helper cells and CD8+ cytotoxic T cells, compared with either AdCMVmIL-12 alone (1.3-fold increase for CD4+ T cells and 1.2-fold increase for CD8+ T cells, P<0.01) or AdhTERTHRP alone (2.1-fold increase for CD4+ T cells and 2.2-fold increase for CD8+ T cells, P <0.01). The apoptotic cells in combination group were significantly increased compared with AdCMVmIL-12 alone group (2.8-fold increase, P<0.01) or AdhTERTHRP alone group (1.6-fold increase, P<0.01). No significant systematic toxicities were observed. Conclusions: Combination gene therapy with AdhTERTHRP/IAA and AdCMVmIL-12 could significantly inhibit tumor growth and improve host survival in LLC model, without significant systemic adverse effects." @default.
- W2589554599 created "2017-03-03" @default.
- W2589554599 creator A5013205871 @default.
- W2589554599 creator A5062547752 @default.
- W2589554599 creator A5064203621 @default.
- W2589554599 creator A5071514312 @default.
- W2589554599 creator A5074004084 @default.
- W2589554599 creator A5075992095 @default.
- W2589554599 creator A5080557200 @default.
- W2589554599 date "2011-05-20" @default.
- W2589554599 modified "2023-09-27" @default.
- W2589554599 title "Gene therapy with tumor-specific promoter mediated suicide gene plus interleukin-12 gene enhanced tumor inhibition and prolonged host survival in a murine model of Lewis lung carcinoma." @default.
- W2589554599 doi "https://doi.org/10.1200/jco.2011.29.15_suppl.e13086" @default.
- W2589554599 hasPublicationYear "2011" @default.
- W2589554599 type Work @default.
- W2589554599 sameAs 2589554599 @default.
- W2589554599 citedByCount "0" @default.
- W2589554599 crossrefType "journal-article" @default.
- W2589554599 hasAuthorship W2589554599A5013205871 @default.
- W2589554599 hasAuthorship W2589554599A5062547752 @default.
- W2589554599 hasAuthorship W2589554599A5064203621 @default.
- W2589554599 hasAuthorship W2589554599A5071514312 @default.
- W2589554599 hasAuthorship W2589554599A5074004084 @default.
- W2589554599 hasAuthorship W2589554599A5075992095 @default.
- W2589554599 hasAuthorship W2589554599A5080557200 @default.
- W2589554599 hasConcept C104317684 @default.
- W2589554599 hasConcept C111599444 @default.
- W2589554599 hasConcept C121608353 @default.
- W2589554599 hasConcept C125593758 @default.
- W2589554599 hasConcept C126322002 @default.
- W2589554599 hasConcept C142724271 @default.
- W2589554599 hasConcept C150903083 @default.
- W2589554599 hasConcept C153911025 @default.
- W2589554599 hasConcept C154317977 @default.
- W2589554599 hasConcept C167672396 @default.
- W2589554599 hasConcept C202751555 @default.
- W2589554599 hasConcept C203014093 @default.
- W2589554599 hasConcept C207001950 @default.
- W2589554599 hasConcept C2776256026 @default.
- W2589554599 hasConcept C2777701055 @default.
- W2589554599 hasConcept C2777847059 @default.
- W2589554599 hasConcept C2779013556 @default.
- W2589554599 hasConcept C34981463 @default.
- W2589554599 hasConcept C502942594 @default.
- W2589554599 hasConcept C55493867 @default.
- W2589554599 hasConcept C71924100 @default.
- W2589554599 hasConcept C86803240 @default.
- W2589554599 hasConcept C8891405 @default.
- W2589554599 hasConcept C94581717 @default.
- W2589554599 hasConceptScore W2589554599C104317684 @default.
- W2589554599 hasConceptScore W2589554599C111599444 @default.
- W2589554599 hasConceptScore W2589554599C121608353 @default.
- W2589554599 hasConceptScore W2589554599C125593758 @default.
- W2589554599 hasConceptScore W2589554599C126322002 @default.
- W2589554599 hasConceptScore W2589554599C142724271 @default.
- W2589554599 hasConceptScore W2589554599C150903083 @default.
- W2589554599 hasConceptScore W2589554599C153911025 @default.
- W2589554599 hasConceptScore W2589554599C154317977 @default.
- W2589554599 hasConceptScore W2589554599C167672396 @default.
- W2589554599 hasConceptScore W2589554599C202751555 @default.
- W2589554599 hasConceptScore W2589554599C203014093 @default.
- W2589554599 hasConceptScore W2589554599C207001950 @default.
- W2589554599 hasConceptScore W2589554599C2776256026 @default.
- W2589554599 hasConceptScore W2589554599C2777701055 @default.
- W2589554599 hasConceptScore W2589554599C2777847059 @default.
- W2589554599 hasConceptScore W2589554599C2779013556 @default.
- W2589554599 hasConceptScore W2589554599C34981463 @default.
- W2589554599 hasConceptScore W2589554599C502942594 @default.
- W2589554599 hasConceptScore W2589554599C55493867 @default.
- W2589554599 hasConceptScore W2589554599C71924100 @default.
- W2589554599 hasConceptScore W2589554599C86803240 @default.
- W2589554599 hasConceptScore W2589554599C8891405 @default.
- W2589554599 hasConceptScore W2589554599C94581717 @default.
- W2589554599 hasIssue "15_suppl" @default.
- W2589554599 hasLocation W25895545991 @default.
- W2589554599 hasOpenAccess W2589554599 @default.
- W2589554599 hasPrimaryLocation W25895545991 @default.
- W2589554599 hasRelatedWork W1969260248 @default.
- W2589554599 hasRelatedWork W2009724739 @default.
- W2589554599 hasRelatedWork W2039798454 @default.
- W2589554599 hasRelatedWork W2061548598 @default.
- W2589554599 hasRelatedWork W2078188006 @default.
- W2589554599 hasRelatedWork W2159233376 @default.
- W2589554599 hasRelatedWork W2163606582 @default.
- W2589554599 hasRelatedWork W2320037565 @default.
- W2589554599 hasRelatedWork W2414303356 @default.
- W2589554599 hasRelatedWork W2589554599 @default.
- W2589554599 hasVolume "29" @default.
- W2589554599 isParatext "false" @default.
- W2589554599 isRetracted "false" @default.
- W2589554599 magId "2589554599" @default.
- W2589554599 workType "article" @default.