Matches in SemOpenAlex for { <https://semopenalex.org/work/W2589713069> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2589713069 abstract "The CRISPR/Cas9 technology has revolutionized gene-editing approaches, by favoring rapid and efficient modifications of gene function, from knock-out to knock-in, and even to silencing or activation of target genes (1,2). In the October 25 th issue of Cell Reports, Gundry et al . (3) describe a novel and efficient method to engineer hematopoietic stem/progenitor cells (HSPCs), of both human and murine origin. In this study, highly efficient disruption of critical genes for myeloid cell development and function, including Dnmt3a , Eed and Suz12 (4), was achieved through virus-free systems. The authors achieve efficient delivery of small guide RNAs (sgRNAs) to murine Cas9-expressing HSPCs by electroporation, with subsequent knock-out of target genes, while maintaining cell viability and colony-forming properties of the edited cells. Targeting of Eed and Suz12 increased proliferation of the edited cells, and their capacity to serially replate, confirming the oncogenic properties of the gene targets. Importantly, the authors report complete virus-free delivery of both Cas9 and sgRNA in human primary T cells and in cord-blood derived HSPCs. By optimizing culture and transfection protocols they could achieve targeting efficiencies of over 80% in these cells. Off-target effects of the sgRNAs were detectable at a very low rate, while multilineage reconstitution capacity of stem cells was maintained." @default.
- W2589713069 created "2017-03-03" @default.
- W2589713069 creator A5040590433 @default.
- W2589713069 creator A5073190879 @default.
- W2589713069 date "2017-02-01" @default.
- W2589713069 modified "2023-09-27" @default.
- W2589713069 title "CRISPR made easy in human and murine hematopoietic precursors" @default.
- W2589713069 doi "https://doi.org/10.21037/12136" @default.
- W2589713069 hasPublicationYear "2017" @default.
- W2589713069 type Work @default.
- W2589713069 sameAs 2589713069 @default.
- W2589713069 citedByCount "0" @default.
- W2589713069 crossrefType "journal-article" @default.
- W2589713069 hasAuthorship W2589713069A5040590433 @default.
- W2589713069 hasAuthorship W2589713069A5073190879 @default.
- W2589713069 hasConcept C104317684 @default.
- W2589713069 hasConcept C109159458 @default.
- W2589713069 hasConcept C111599444 @default.
- W2589713069 hasConcept C119056186 @default.
- W2589713069 hasConcept C132455925 @default.
- W2589713069 hasConcept C135983454 @default.
- W2589713069 hasConcept C144501496 @default.
- W2589713069 hasConcept C166703698 @default.
- W2589713069 hasConcept C174510640 @default.
- W2589713069 hasConcept C186852380 @default.
- W2589713069 hasConcept C189819185 @default.
- W2589713069 hasConcept C201750760 @default.
- W2589713069 hasConcept C2780914630 @default.
- W2589713069 hasConcept C28328180 @default.
- W2589713069 hasConcept C54009773 @default.
- W2589713069 hasConcept C54355233 @default.
- W2589713069 hasConcept C67705224 @default.
- W2589713069 hasConcept C86803240 @default.
- W2589713069 hasConcept C95444343 @default.
- W2589713069 hasConcept C97702854 @default.
- W2589713069 hasConcept C98108389 @default.
- W2589713069 hasConceptScore W2589713069C104317684 @default.
- W2589713069 hasConceptScore W2589713069C109159458 @default.
- W2589713069 hasConceptScore W2589713069C111599444 @default.
- W2589713069 hasConceptScore W2589713069C119056186 @default.
- W2589713069 hasConceptScore W2589713069C132455925 @default.
- W2589713069 hasConceptScore W2589713069C135983454 @default.
- W2589713069 hasConceptScore W2589713069C144501496 @default.
- W2589713069 hasConceptScore W2589713069C166703698 @default.
- W2589713069 hasConceptScore W2589713069C174510640 @default.
- W2589713069 hasConceptScore W2589713069C186852380 @default.
- W2589713069 hasConceptScore W2589713069C189819185 @default.
- W2589713069 hasConceptScore W2589713069C201750760 @default.
- W2589713069 hasConceptScore W2589713069C2780914630 @default.
- W2589713069 hasConceptScore W2589713069C28328180 @default.
- W2589713069 hasConceptScore W2589713069C54009773 @default.
- W2589713069 hasConceptScore W2589713069C54355233 @default.
- W2589713069 hasConceptScore W2589713069C67705224 @default.
- W2589713069 hasConceptScore W2589713069C86803240 @default.
- W2589713069 hasConceptScore W2589713069C95444343 @default.
- W2589713069 hasConceptScore W2589713069C97702854 @default.
- W2589713069 hasConceptScore W2589713069C98108389 @default.
- W2589713069 hasIssue "1" @default.
- W2589713069 hasLocation W25897130691 @default.
- W2589713069 hasOpenAccess W2589713069 @default.
- W2589713069 hasPrimaryLocation W25897130691 @default.
- W2589713069 hasRelatedWork W2318717934 @default.
- W2589713069 hasRelatedWork W2563601716 @default.
- W2589713069 hasRelatedWork W2576159092 @default.
- W2589713069 hasRelatedWork W2769059387 @default.
- W2589713069 hasRelatedWork W2790984880 @default.
- W2589713069 hasRelatedWork W2810487964 @default.
- W2589713069 hasRelatedWork W2897118504 @default.
- W2589713069 hasRelatedWork W2919875709 @default.
- W2589713069 hasRelatedWork W2950101654 @default.
- W2589713069 hasRelatedWork W2966022850 @default.
- W2589713069 hasRelatedWork W2967892870 @default.
- W2589713069 hasRelatedWork W2977551986 @default.
- W2589713069 hasRelatedWork W2978300467 @default.
- W2589713069 hasRelatedWork W3013645106 @default.
- W2589713069 hasRelatedWork W3033044611 @default.
- W2589713069 hasRelatedWork W3040250801 @default.
- W2589713069 hasRelatedWork W3096470472 @default.
- W2589713069 hasRelatedWork W3117761089 @default.
- W2589713069 hasRelatedWork W3127333316 @default.
- W2589713069 hasRelatedWork W45599239 @default.
- W2589713069 hasVolume "6" @default.
- W2589713069 isParatext "false" @default.
- W2589713069 isRetracted "false" @default.
- W2589713069 magId "2589713069" @default.
- W2589713069 workType "article" @default.