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• W2589733533 abstract "Summary Tuberculosis ( TB ), the leading cause of death among infectious diseases worldwide, is caused by Mycobacterium tuberculosis ( M. tuberculosis ). Early accurate diagnosis means earlier prevention, treatment and control of TB . To confirm efficient diagnostic antigens for M. tuberculosis , the serodiagnosis value of four recombinant proteins including Rv0220, Rv2958c, Rv2994 and Rv3347c was evaluated in this study. The specificities and sensitivities of four recombinant proteins were determined based on enzyme‐linked immunosorbent assay ( ELISA ) by screening sera from smear‐positive pulmonary TB patients ( n = 92), uninfected individuals ( n = 60) and patients with Mycoplasma pneumoniae ( n = 32) that potentially cross‐react with M. tuberculosis . The ELISA s showed that Rv0220, Rv2958c, Rv2994 and Rv3347c exhibited high specificities and sensitivities in detecting immunoglobulin G (IgG) antibody, with 98.3/91.3%, 91.7/85.9%, 93.3/89.1% and 93.3/80.4% respectively. According to the receiver‐operating characteristic ( ROC ) analysis, the area under the ROC of the target proteins was 0.988, 0.969, 0.929 and 0.945 respectively. Western blot was established to evaluate the immunoreactivities of target proteins to mice and human sera. Results demonstrated that Rv0220, Rv2958c, Rv2994 and Rv3347c could specifically recognize TB ‐positive sera and the sera of mice immunized with the corresponding protein. Thus, Rv0220, Rv2958c, Rv2994 and Rv3347c were valuable potential diagnostic antigens for M. tuberculosis ." @default.
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• W2589733533 date "2017-02-20" @default.
• W2589733533 modified "2023-09-22" @default.
• W2589733533 title "Evaluation of Rv0220, Rv2958c, Rv2994 and Rv3347c of <i>Mycobacterium tuberculosis</i> for serodiagnosis of tuberculosis" @default.
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• W2589733533 doi "https://doi.org/10.1111/1751-7915.12697" @default.
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