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- W2589881200 abstract "High performance thin layer chromatographic method has been developed for the simultaneous determination of Propranolol hydrochloride and Hydrochlorothiazide from bulk and formulations. Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60F254, with chloroform: ethyl acetate: methanol 4: 4: 2 (v/v/v) as mobile phase. Detection was performed densitometrically at 274 nm. The Rf of Propranolol hydrochloride and Hydrochlorothiazide were 0.27± 0.02 and 0.56 ± 0.02, respectively. Parameters such as linearity, precision, accuracy, specificity and robustness are studied as reported in the ICH guidelines. Linearity was observed in the concentration range of 160-960 ng/band for Propranolol hydrochloride and 100-600 ng/band for Hydrochlorothiazide. The mean recoveries obtained for Propranolol hydrochloride and Hydrochlorothiazide were 99.63 % and 99.15 % respectively. Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of Propranolol hydrochloride and Hydrochlorothiazide." @default.
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- W2589881200 date "2016-01-01" @default.
- W2589881200 modified "2023-09-24" @default.
- W2589881200 title "Simultaneous analysis of propranolol HCl and hydrochlorothiazide byHPTLC" @default.
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