Matches in SemOpenAlex for { <https://semopenalex.org/work/W2590149512> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2590149512 abstract "Heart failure comprises of clinically distinct inciting causes but a consistent pattern of change in myocardial gene expression supports the hypothesis that unifying biochemical mechanisms underlie disease progression. The recent RNA-seq revolution has enabled whole transcriptome profiling, using deep-sequencing technologies. Up to 70% of the genome is now known to be transcribed into RNA, a significant proportion of which is long non-coding RNAs (lncRNAs), defined as polyribonucleotides of ≥200 nucleotides. This project aims to discover whether the myocardium expression of lncRNAs changes in the failing heart. Paired end RNA-seq from a 300-400bp library of ‘stretched’ mouse myocyte total RNA was carried out to generate 76-mer sequence reads. Mechanically stretching myocytes with equibiaxial stretch apparatus mimics pathological hypertrophy in the heart. Transcripts were assembled and aligned to reference genome mm9 (UCSC), abundance determined and differential expression of novel transcripts and alternative splice variants were compared with that of control (non-stretched) mouse myocytes. Five novel transcripts have been identified in our RNA-seq that are differentially expressed in stretched myocytes compared with non-stretched. These are regions of the genome that are currently unannotated and potentially are transcribed into non-coding RNAs. Roles of known lncRNAs include control of gene expression, either by direct interaction with complementary regions of the genome or association with chromatin remodelling complexes which act on the epigenome.Changes in expression of genes which contribute to the deterioration of the failing heart could be due to the actions of these novel lncRNAs, immediately suggesting a target for new pharmaceuticals. Changes in the expression of these novel transcripts will be validated in a larger sample size of stretched myocytes vs non-stretched myocytes as well as in the hearts of transverse aortic constriction (TAC) mice vs Sham (surgical procedure without the aortic banding). In vivo investigations will then be carried out, using siLNA antisense technology to silence novel lncRNAs in mice." @default.
- W2590149512 created "2017-03-03" @default.
- W2590149512 creator A5015161628 @default.
- W2590149512 creator A5043139833 @default.
- W2590149512 creator A5072865995 @default.
- W2590149512 creator A5078347427 @default.
- W2590149512 creator A5086330332 @default.
- W2590149512 date "2012-08-03" @default.
- W2590149512 modified "2023-09-28" @default.
- W2590149512 title "Abstract 286: Long Noncoding RNAs Are Differentially Expressed in Heart Failure" @default.
- W2590149512 doi "https://doi.org/10.1161/res.111.suppl_1.a286" @default.
- W2590149512 hasPublicationYear "2012" @default.
- W2590149512 type Work @default.
- W2590149512 sameAs 2590149512 @default.
- W2590149512 citedByCount "0" @default.
- W2590149512 crossrefType "journal-article" @default.
- W2590149512 hasAuthorship W2590149512A5015161628 @default.
- W2590149512 hasAuthorship W2590149512A5043139833 @default.
- W2590149512 hasAuthorship W2590149512A5072865995 @default.
- W2590149512 hasAuthorship W2590149512A5078347427 @default.
- W2590149512 hasAuthorship W2590149512A5086330332 @default.
- W2590149512 hasConcept C104317684 @default.
- W2590149512 hasConcept C107397762 @default.
- W2590149512 hasConcept C117717151 @default.
- W2590149512 hasConcept C141231307 @default.
- W2590149512 hasConcept C150194340 @default.
- W2590149512 hasConcept C162317418 @default.
- W2590149512 hasConcept C18431079 @default.
- W2590149512 hasConcept C190727270 @default.
- W2590149512 hasConcept C194993378 @default.
- W2590149512 hasConcept C54355233 @default.
- W2590149512 hasConcept C54458228 @default.
- W2590149512 hasConcept C62203573 @default.
- W2590149512 hasConcept C67705224 @default.
- W2590149512 hasConcept C70721500 @default.
- W2590149512 hasConcept C83640560 @default.
- W2590149512 hasConcept C86803240 @default.
- W2590149512 hasConcept C95444343 @default.
- W2590149512 hasConceptScore W2590149512C104317684 @default.
- W2590149512 hasConceptScore W2590149512C107397762 @default.
- W2590149512 hasConceptScore W2590149512C117717151 @default.
- W2590149512 hasConceptScore W2590149512C141231307 @default.
- W2590149512 hasConceptScore W2590149512C150194340 @default.
- W2590149512 hasConceptScore W2590149512C162317418 @default.
- W2590149512 hasConceptScore W2590149512C18431079 @default.
- W2590149512 hasConceptScore W2590149512C190727270 @default.
- W2590149512 hasConceptScore W2590149512C194993378 @default.
- W2590149512 hasConceptScore W2590149512C54355233 @default.
- W2590149512 hasConceptScore W2590149512C54458228 @default.
- W2590149512 hasConceptScore W2590149512C62203573 @default.
- W2590149512 hasConceptScore W2590149512C67705224 @default.
- W2590149512 hasConceptScore W2590149512C70721500 @default.
- W2590149512 hasConceptScore W2590149512C83640560 @default.
- W2590149512 hasConceptScore W2590149512C86803240 @default.
- W2590149512 hasConceptScore W2590149512C95444343 @default.
- W2590149512 hasIssue "suppl_1" @default.
- W2590149512 hasLocation W25901495121 @default.
- W2590149512 hasOpenAccess W2590149512 @default.
- W2590149512 hasPrimaryLocation W25901495121 @default.
- W2590149512 hasRelatedWork W1483760785 @default.
- W2590149512 hasRelatedWork W1982353721 @default.
- W2590149512 hasRelatedWork W1985256087 @default.
- W2590149512 hasRelatedWork W2090296648 @default.
- W2590149512 hasRelatedWork W2097071638 @default.
- W2590149512 hasRelatedWork W2107766124 @default.
- W2590149512 hasRelatedWork W2157286838 @default.
- W2590149512 hasRelatedWork W2509529079 @default.
- W2590149512 hasRelatedWork W2516250146 @default.
- W2590149512 hasRelatedWork W2594790402 @default.
- W2590149512 hasRelatedWork W2741674059 @default.
- W2590149512 hasRelatedWork W2742692124 @default.
- W2590149512 hasRelatedWork W2768775309 @default.
- W2590149512 hasRelatedWork W2789569485 @default.
- W2590149512 hasRelatedWork W2794379545 @default.
- W2590149512 hasRelatedWork W2890076404 @default.
- W2590149512 hasRelatedWork W3036746316 @default.
- W2590149512 hasRelatedWork W3094992383 @default.
- W2590149512 hasRelatedWork W3207757058 @default.
- W2590149512 hasRelatedWork W3125016605 @default.
- W2590149512 hasVolume "111" @default.
- W2590149512 isParatext "false" @default.
- W2590149512 isRetracted "false" @default.
- W2590149512 magId "2590149512" @default.
- W2590149512 workType "article" @default.