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- W2590204611 abstract "To investigate the presence of interactions between DNAJB13 and HK1.The open reading frame of Dnajb13 gene was amplified from mouse testis cDNA by PCR. The PCR products were then inserted into pGEX-4T-1 vector after double digestion and identified by sequencing. The recombinant plasmids were transformated into competent DH5a cells, and the fusion protein was expressed with IPTG induction. SDS-PAGE Coomassie brilliant blue staining and Western blot analysis were used to detect the fusion protein expression. The protein precipitated by GST-DNAJB13 in GST pull down assay was detected by Western blotting.The recombinant plasmid pGEX-4T-1-Dnajb13 was successfully constructed and verified. E.coli transformed with the recombinant plasmid expressed abundant fusion protein. GST pull down assay showed interactions between DNAJB13 and HK1.DNAJB13 interacts with HK1 in mouse testis and probably participates in spermatogenesis and the regulation of sperm motility." @default.
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- W2590204611 date "2016-12-20" @default.
- W2590204611 modified "2023-09-23" @default.
- W2590204611 title "[Interaction of DNAJB13 with HK1 in mouse]." @default.
- W2590204611 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27998865" @default.
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