Matches in SemOpenAlex for { <https://semopenalex.org/work/W2590785909> ?p ?o ?g. }
- W2590785909 endingPage "44" @default.
- W2590785909 startingPage "37" @default.
- W2590785909 abstract "Nitrile hydratase (NHase) has attracted substantial attention for industrial applications to produce large-scale amides. Several NHases have been investigated for functional expression in Escherichia coli (E. coli). A Fe-type NHase was obtained from an acetamiprid-degrading bacterium, Pseudoxanthomonas sp. AAP-7 and functionally expressed in E. coli BL21 (DE3). No significant NHase activity was detected from the E. coli expressing either the NHase gene alone or NHase and P46K genes transcribed as one unit. Purified recombinant NHase, co-expressed with P46K on two separate plasmids, exhibited the maximal enzyme activity. Furthermore, a GST tag attached to the N-terminus of α subunit resulted in a slight increase in the solubility and stability of NHase compared with a His tag at the C-terminus of β subunit. When co-expressed with the chaperones GroEL-GroES, the yield of the soluble recombinant NHase was improved substantially, while a small decrease in NHase activity was observed. The putative activator P46K was strictly required for production of the recombinant NHase for full enzyme activity, although the chaperones GroEL-GroES appeared to assist NHase to fold properly. This study of the expression of a fully active Fe-type NHase would provide another example to enhance our understanding of NHase biosynthesis." @default.
- W2590785909 created "2017-03-03" @default.
- W2590785909 creator A5000442440 @default.
- W2590785909 creator A5003759585 @default.
- W2590785909 creator A5035276566 @default.
- W2590785909 creator A5040905727 @default.
- W2590785909 creator A5075391403 @default.
- W2590785909 creator A5084111615 @default.
- W2590785909 date "2017-05-01" @default.
- W2590785909 modified "2023-10-18" @default.
- W2590785909 title "Chaperone-assisted maturation of the recombinant Fe-type nitrile hydratase is insufficient for fully active expression in Escherichia coli" @default.
- W2590785909 cites W1551390803 @default.
- W2590785909 cites W1583668498 @default.
- W2590785909 cites W1969932115 @default.
- W2590785909 cites W1983777334 @default.
- W2590785909 cites W1990072931 @default.
- W2590785909 cites W1992100606 @default.
- W2590785909 cites W2007062211 @default.
- W2590785909 cites W2009374170 @default.
- W2590785909 cites W2021777552 @default.
- W2590785909 cites W2028262399 @default.
- W2590785909 cites W2039360079 @default.
- W2590785909 cites W2040526720 @default.
- W2590785909 cites W2044863181 @default.
- W2590785909 cites W2050148297 @default.
- W2590785909 cites W2053390725 @default.
- W2590785909 cites W2061120397 @default.
- W2590785909 cites W2066005643 @default.
- W2590785909 cites W2073385989 @default.
- W2590785909 cites W2076330087 @default.
- W2590785909 cites W2084325856 @default.
- W2590785909 cites W2090601506 @default.
- W2590785909 cites W2095188041 @default.
- W2590785909 cites W2141459263 @default.
- W2590785909 cites W2146805005 @default.
- W2590785909 cites W2149020682 @default.
- W2590785909 cites W2152207030 @default.
- W2590785909 cites W2158989718 @default.
- W2590785909 cites W2164079892 @default.
- W2590785909 cites W2169023201 @default.
- W2590785909 cites W2218020728 @default.
- W2590785909 cites W2287166990 @default.
- W2590785909 cites W2334049226 @default.
- W2590785909 cites W2509512978 @default.
- W2590785909 cites W4232605987 @default.
- W2590785909 doi "https://doi.org/10.1016/j.procbio.2017.02.018" @default.
- W2590785909 hasPublicationYear "2017" @default.
- W2590785909 type Work @default.
- W2590785909 sameAs 2590785909 @default.
- W2590785909 citedByCount "5" @default.
- W2590785909 countsByYear W25907859092019 @default.
- W2590785909 countsByYear W25907859092020 @default.
- W2590785909 countsByYear W25907859092021 @default.
- W2590785909 countsByYear W25907859092023 @default.
- W2590785909 crossrefType "journal-article" @default.
- W2590785909 hasAuthorship W2590785909A5000442440 @default.
- W2590785909 hasAuthorship W2590785909A5003759585 @default.
- W2590785909 hasAuthorship W2590785909A5035276566 @default.
- W2590785909 hasAuthorship W2590785909A5040905727 @default.
- W2590785909 hasAuthorship W2590785909A5075391403 @default.
- W2590785909 hasAuthorship W2590785909A5084111615 @default.
- W2590785909 hasConcept C104317684 @default.
- W2590785909 hasConcept C142724271 @default.
- W2590785909 hasConcept C153911025 @default.
- W2590785909 hasConcept C178790620 @default.
- W2590785909 hasConcept C181199279 @default.
- W2590785909 hasConcept C185592680 @default.
- W2590785909 hasConcept C2775962898 @default.
- W2590785909 hasConcept C2779122190 @default.
- W2590785909 hasConcept C2779240715 @default.
- W2590785909 hasConcept C40767141 @default.
- W2590785909 hasConcept C547475151 @default.
- W2590785909 hasConcept C55493867 @default.
- W2590785909 hasConcept C71924100 @default.
- W2590785909 hasConcept C86803240 @default.
- W2590785909 hasConcept C95444343 @default.
- W2590785909 hasConceptScore W2590785909C104317684 @default.
- W2590785909 hasConceptScore W2590785909C142724271 @default.
- W2590785909 hasConceptScore W2590785909C153911025 @default.
- W2590785909 hasConceptScore W2590785909C178790620 @default.
- W2590785909 hasConceptScore W2590785909C181199279 @default.
- W2590785909 hasConceptScore W2590785909C185592680 @default.
- W2590785909 hasConceptScore W2590785909C2775962898 @default.
- W2590785909 hasConceptScore W2590785909C2779122190 @default.
- W2590785909 hasConceptScore W2590785909C2779240715 @default.
- W2590785909 hasConceptScore W2590785909C40767141 @default.
- W2590785909 hasConceptScore W2590785909C547475151 @default.
- W2590785909 hasConceptScore W2590785909C55493867 @default.
- W2590785909 hasConceptScore W2590785909C71924100 @default.
- W2590785909 hasConceptScore W2590785909C86803240 @default.
- W2590785909 hasConceptScore W2590785909C95444343 @default.
- W2590785909 hasLocation W25907859091 @default.
- W2590785909 hasOpenAccess W2590785909 @default.
- W2590785909 hasPrimaryLocation W25907859091 @default.
- W2590785909 hasRelatedWork W1969150026 @default.
- W2590785909 hasRelatedWork W2016628627 @default.
- W2590785909 hasRelatedWork W2074541085 @default.
- W2590785909 hasRelatedWork W2094329052 @default.