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• W2591337778 startingPage "113" @default.
• W2591337778 abstract "Abstract The rapidity with which epithelial cells cover a wound surface helps determine whether scarring or scar-less healing results. As methylene blue is a vital dye that is absorbed by damaged tissue but not undamaged epidermis, it can be used to assess wound closure. We sought to develop a quantitative methylene blue exclusion assay to estimate the timeframe for re-epithelialization in regenerating appendages in zebrafish and axolotls, two classic model systems of regeneration. Following application of methylene blue to the amputation plane and extensive washing, the regenerating tail was imaged in vivo until staining was no longer visible. The percent area of the amputation plane positive for methylene blue, representing the area of the amputation plane not yet re-epithelialized, was measured for each time point. The loss of methylene blue occurred rapidly, within ~2.5 h in larval and juvenile axolotls and <1 h in adult zebrafish, consistent with high rates of re-epithelialization in these models of regeneration. The assay allows simple, rapid estimation of the time course for regenerative re-epithelialization without affecting subsequent regenerative ability. This technique will permit comparison of re-epithelialization across different strains and stages, as well as under the influence of various pharmacological inhibitors that affect regeneration." @default.
• W2591337778 created "2017-03-03" @default.
• W2591337778 creator A5067652889 @default.
• W2591337778 creator A5068144931 @default.
• W2591337778 date "2017-02-01" @default.
• W2591337778 modified "2023-09-27" @default.
• W2591337778 title "Methylene Blue Assay for Estimation of Regenerative Re-Epithelialization<i>In Vivo</i>" @default.
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• W2591337778 doi "https://doi.org/10.1017/s1431927617000101" @default.
• W2591337778 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28228166" @default.
• W2591337778 hasPublicationYear "2017" @default.
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