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• W2591517847 abstract "Increasing interest in protein immobilization on surfaces has heightened the need for techniques enabling layer-by-layer protein attachment. Here, we report a technique for controlling enzyme-mediated immobilization of layers of protein on the surface using a genetically encoded protecting group. An enterokinase-cleavable peptide sequence was inserted at the N-terminus of bifunctional fluorescent proteins containing Sortase A substrate recognition tags at both ends to control Sortase A-mediated protein immobilization on the surface layer-by-layer. Efficient, sequential immobilization of a second layer of protein using Sortase A required removal of the N-terminal protecting group, suggesting the method enables multilayer synthesis using cyclic deprotection and coupling steps. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:824-831, 2017." @default.
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• W2591517847 date "2017-03-11" @default.
• W2591517847 modified "2023-10-18" @default.
• W2591517847 title "F ine‐tuning sortase‐mediated immobilization of protein layers on surfaces using sequential deprotection and coupling" @default.
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• W2591517847 doi "https://doi.org/10.1002/btpr.2449" @default.
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