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- W2591570310 abstract "Abstract A label-free dual-cycle targets recycling strategy is firstly proposed for ultrasensitive detection of Hg 2+ based on the Hg 2+ -triggered toehold binding, exonuclease III-assisted dual-cycle targets recycling signal amplification and hemin/G-quadruplex (DNAzyme) signal amplification. In the presence of Hg 2+ , the matched hybridization of Hg 2+ discrimination probe and the duplex DNA probe by T–Hg 2+ –T coordination triggers the Exo III-assisted DNA cleavages that release Hg 2+ , Hg 2+ discrimination probe, G-quadruplex forming DNA probe and a new target DNA fragment (I-region). The released Hg 2+ (cycle 1) and the new target DNA (cycle 2) are recycled. The Exo III-assisted dual-cycle targets recycling amplification triggered by ultratrace amounts of Hg 2+ produces numerous quadruplex-forming sequences that bind hemin to yield G-quadruplex–hemin DNAzymes, which efficiently catalyze the H 2 O 2 -mediated oxidation of the ABTS 2− to the colored products. This method exhibits a high sensitivity toward Hg 2+ with a detection limit of 0.05 pmol/L, and the calibration curve is linear in the range of 0.05 pmol/L to 100 pmol/L ( R 2 = 0.9905). This proposed strategy displayed excellent sensitivity, simplicity, low-cost and remarkable specificity, and was successfully implemented for colorimetric detection of Hg 2+ in tap water, lake water and Xiangjiang River water samples with good recovery and accuracy." @default.
- W2591570310 created "2017-03-03" @default.
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- W2591570310 date "2017-07-01" @default.
- W2591570310 modified "2023-10-14" @default.
- W2591570310 title "Hg2+-triggered exonuclease III-assisted dual-cycle targets recycling amplification for label-free and ultrasensitive colorimetric detection of Hg2+" @default.
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- W2591570310 doi "https://doi.org/10.1016/j.snb.2017.02.103" @default.
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