Matches in SemOpenAlex for { <https://semopenalex.org/work/W2591592225> ?p ?o ?g. }
- W2591592225 abstract "ABSTRACT Enterococcus faecium is one of the primary causes of nosocomial infections. Disinfectants are commonly used to prevent infections with multidrug-resistant E. faecium in hospitals. Worryingly, E. faecium strains that exhibit tolerance to disinfectants have already been described. We aimed to identify and characterize E. faecium genes that contribute to tolerance to the disinfectant chlorhexidine (CHX). We used a transposon mutant library, constructed in a multidrug-resistant E. faecium bloodstream isolate, to perform a genome-wide screen to identify genetic determinants involved in tolerance to CHX. We identified a putative two-component system (2CS), composed of a putative sensor histidine kinase (ChtS) and a cognate DNA-binding response regulator (ChtR), which contributed to CHX tolerance in E. faecium . Targeted chtR and chtS deletion mutants exhibited compromised growth in the presence of CHX. Growth of the chtR and chtS mutants was also affected in the presence of the antibiotic bacitracin. The CHX- and bacitracin-tolerant phenotype of E. faecium E1162 was linked to a unique, nonsynonymous single nucleotide polymorphism in chtR . Transmission electron microscopy showed that upon challenge with CHX, the Δ chtR and Δ chtS mutants failed to divide properly and formed long chains. Normal growth and cell morphology were restored when the mutations were complemented in trans . Morphological abnormalities were also observed upon exposure of the Δ chtR and Δ chtS mutants to bacitracin. The tolerance to both chlorhexidine and bacitracin provided by ChtRS in E. faecium highlights the overlap between responses to disinfectants and antibiotics and the potential for the development of cross-tolerance for these classes of antimicrobials." @default.
- W2591592225 created "2017-03-16" @default.
- W2591592225 creator A5019726320 @default.
- W2591592225 creator A5021655542 @default.
- W2591592225 creator A5024002499 @default.
- W2591592225 creator A5032000937 @default.
- W2591592225 creator A5032590471 @default.
- W2591592225 creator A5040732518 @default.
- W2591592225 creator A5046314876 @default.
- W2591592225 creator A5048223661 @default.
- W2591592225 creator A5055970684 @default.
- W2591592225 creator A5071940038 @default.
- W2591592225 creator A5072187427 @default.
- W2591592225 date "2017-05-01" @default.
- W2591592225 modified "2023-10-14" @default.
- W2591592225 title "The Two-Component System ChtRS Contributes to Chlorhexidine Tolerance in Enterococcus faecium" @default.
- W2591592225 cites W1227555702 @default.
- W2591592225 cites W153768368 @default.
- W2591592225 cites W1587896079 @default.
- W2591592225 cites W1887482410 @default.
- W2591592225 cites W1966309728 @default.
- W2591592225 cites W1969383727 @default.
- W2591592225 cites W1969828336 @default.
- W2591592225 cites W1972389906 @default.
- W2591592225 cites W1974222802 @default.
- W2591592225 cites W1976667730 @default.
- W2591592225 cites W1990152860 @default.
- W2591592225 cites W1992601854 @default.
- W2591592225 cites W1999755040 @default.
- W2591592225 cites W2001530132 @default.
- W2591592225 cites W2009914378 @default.
- W2591592225 cites W2010893628 @default.
- W2591592225 cites W2012529887 @default.
- W2591592225 cites W2035388141 @default.
- W2591592225 cites W2035636470 @default.
- W2591592225 cites W2046368318 @default.
- W2591592225 cites W2055351105 @default.
- W2591592225 cites W2057460911 @default.
- W2591592225 cites W2061020866 @default.
- W2591592225 cites W2071731040 @default.
- W2591592225 cites W2072118486 @default.
- W2591592225 cites W2073444517 @default.
- W2591592225 cites W2074273917 @default.
- W2591592225 cites W2078994321 @default.
- W2591592225 cites W2091299154 @default.
- W2591592225 cites W2092214414 @default.
- W2591592225 cites W2095459919 @default.
- W2591592225 cites W2103599303 @default.
- W2591592225 cites W2109613442 @default.
- W2591592225 cites W2110343075 @default.
- W2591592225 cites W2110769734 @default.
- W2591592225 cites W2117491496 @default.
- W2591592225 cites W2119654574 @default.
- W2591592225 cites W2123543206 @default.
- W2591592225 cites W2125584125 @default.
- W2591592225 cites W2130351906 @default.
- W2591592225 cites W2131709544 @default.
- W2591592225 cites W2132963724 @default.
- W2591592225 cites W2137167776 @default.
- W2591592225 cites W2137742056 @default.
- W2591592225 cites W2138105880 @default.
- W2591592225 cites W2138606009 @default.
- W2591592225 cites W2144221610 @default.
- W2591592225 cites W2144227498 @default.
- W2591592225 cites W2147792727 @default.
- W2591592225 cites W2153842659 @default.
- W2591592225 cites W2157979510 @default.
- W2591592225 cites W2163750088 @default.
- W2591592225 cites W2165595509 @default.
- W2591592225 cites W2178540098 @default.
- W2591592225 cites W2180695509 @default.
- W2591592225 cites W2218892404 @default.
- W2591592225 cites W2264366671 @default.
- W2591592225 cites W2398608465 @default.
- W2591592225 cites W2516641646 @default.
- W2591592225 doi "https://doi.org/10.1128/aac.02122-16" @default.
- W2591592225 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5404517" @default.
- W2591592225 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28242664" @default.
- W2591592225 hasPublicationYear "2017" @default.
- W2591592225 type Work @default.
- W2591592225 sameAs 2591592225 @default.
- W2591592225 citedByCount "33" @default.
- W2591592225 countsByYear W25915922252016 @default.
- W2591592225 countsByYear W25915922252017 @default.
- W2591592225 countsByYear W25915922252018 @default.
- W2591592225 countsByYear W25915922252019 @default.
- W2591592225 countsByYear W25915922252020 @default.
- W2591592225 countsByYear W25915922252021 @default.
- W2591592225 countsByYear W25915922252022 @default.
- W2591592225 countsByYear W25915922252023 @default.
- W2591592225 crossrefType "journal-article" @default.
- W2591592225 hasAuthorship W2591592225A5019726320 @default.
- W2591592225 hasAuthorship W2591592225A5021655542 @default.
- W2591592225 hasAuthorship W2591592225A5024002499 @default.
- W2591592225 hasAuthorship W2591592225A5032000937 @default.
- W2591592225 hasAuthorship W2591592225A5032590471 @default.
- W2591592225 hasAuthorship W2591592225A5040732518 @default.
- W2591592225 hasAuthorship W2591592225A5046314876 @default.
- W2591592225 hasAuthorship W2591592225A5048223661 @default.
- W2591592225 hasAuthorship W2591592225A5055970684 @default.