FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2591610528> ?p ?o ?g. }

• W2591610528 endingPage "1553" @default.
• W2591610528 startingPage "1553" @default.
• W2591610528 abstract "Purpose: To establish and evaluate a new multiplex solid-phase strip polymerase chain reaction (strip PCR) for concurrent detection of common ocular infectious disease pathogens. Methods: A new multiplex strip PCR was established to detect 24 common ocular infectious disease pathogens: herpes simplex virus (HSV) 1, HSV2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus (HHV) 6, HHV7, HHV8, human T-cell lymphotropic virus (HTLV)-1, adenovirus, Mycobacterium tuberculosis, Treponema pallidum, Propionibacterium acnes (P. acnes), bacterial 16S ribosomal RNA (rRNA), Candida species (Candida sp.), C. glabrata, C. krusei, Aspergillus, Fusarium, fungal 28S rRNA, Toxoplasma (T. gondii), Toxocara, Chlamydia trachomatis (C. trachomatis), and Acanthamoeba. Strip PCR was tested with a negative control (distilled water) and standard positive control DNA. Cutoffs of quantification cycle (Cq) values were determined with noninfectious ocular samples to avoid false-positives caused by contamination with P. acnes, bacterial 16S, and fungal 28S from reagents and ocular surfaces. A pilot study to evaluate the strip PCR was performed using infectious ocular samples (aqueous humor, vitreous, cornea, and tears) by strip PCR and previously developed capillary-type multiplex PCR and quantitative real-time PCR (qPCR). Results: Strip PCR was verified with negative and positive controls. Strip PCR rapidly detected HSV1, HSV2, VZV, EBV, CMV, HHV6, HHV7, HTLV-1, adenovirus, P. acnes, bacterial 16S, Candida sp., C. glabrata, Aspergillus, fungal 28S, T. gondii, C. trachomatis, and Acanthamoeba in patient samples. The sensitivity was comparable to that of qPCR. Conclusions: Our novel strip PCR assay is a simple, rapid, and high-sensitivity method for detecting ocular infectious disease pathogens." @default.
• W2591610528 created "2017-03-16" @default.
• W2591610528 creator A5013615484 @default.
• W2591610528 creator A5017008708 @default.
• W2591610528 creator A5019063960 @default.
• W2591610528 creator A5029122307 @default.
• W2591610528 creator A5030655388 @default.
• W2591610528 creator A5031396339 @default.
• W2591610528 creator A5040963064 @default.
• W2591610528 creator A5046537981 @default.
• W2591610528 creator A5071116694 @default.
• W2591610528 creator A5084495215 @default.
• W2591610528 date "2017-03-10" @default.
• W2591610528 modified "2023-10-17" @default.
• W2591610528 title "Establishment of Multiplex Solid-Phase Strip PCR Test for Detection of 24 Ocular Infectious Disease Pathogens" @default.
• W2591610528 cites W1989464120 @default.
• W2591610528 cites W1994617816 @default.
• W2591610528 cites W2005757972 @default.
• W2591610528 cites W2021096912 @default.
• W2591610528 cites W2038981357 @default.
• W2591610528 cites W2049018136 @default.
• W2591610528 cites W2049268191 @default.
• W2591610528 cites W2050717506 @default.
• W2591610528 cites W2067658693 @default.
• W2591610528 cites W2068136640 @default.
• W2591610528 cites W2083502458 @default.
• W2591610528 cites W2086240162 @default.
• W2591610528 cites W2100513280 @default.
• W2591610528 cites W2111206049 @default.
• W2591610528 cites W2118853478 @default.
• W2591610528 cites W2123320007 @default.
• W2591610528 cites W2125584715 @default.
• W2591610528 cites W2134276568 @default.
• W2591610528 doi "https://doi.org/10.1167/iovs.16-20556" @default.
• W2591610528 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28282487" @default.
• W2591610528 hasPublicationYear "2017" @default.
• W2591610528 type Work @default.
• W2591610528 sameAs 2591610528 @default.
• W2591610528 citedByCount "36" @default.
• W2591610528 countsByYear W25916105282018 @default.
• W2591610528 countsByYear W25916105282019 @default.
• W2591610528 countsByYear W25916105282020 @default.
• W2591610528 countsByYear W25916105282021 @default.
• W2591610528 countsByYear W25916105282022 @default.
• W2591610528 countsByYear W25916105282023 @default.
• W2591610528 crossrefType "journal-article" @default.
• W2591610528 hasAuthorship W2591610528A5013615484 @default.
• W2591610528 hasAuthorship W2591610528A5017008708 @default.
• W2591610528 hasAuthorship W2591610528A5019063960 @default.
• W2591610528 hasAuthorship W2591610528A5029122307 @default.
• W2591610528 hasAuthorship W2591610528A5030655388 @default.
• W2591610528 hasAuthorship W2591610528A5031396339 @default.
• W2591610528 hasAuthorship W2591610528A5040963064 @default.
• W2591610528 hasAuthorship W2591610528A5046537981 @default.
• W2591610528 hasAuthorship W2591610528A5071116694 @default.
• W2591610528 hasAuthorship W2591610528A5084495215 @default.
• W2591610528 hasBestOaLocation W25916105281 @default.
• W2591610528 hasConcept C104317684 @default.
• W2591610528 hasConcept C159047783 @default.
• W2591610528 hasConcept C2522874641 @default.
• W2591610528 hasConcept C2777391075 @default.
• W2591610528 hasConcept C2781188995 @default.
• W2591610528 hasConcept C2781196997 @default.
• W2591610528 hasConcept C49105822 @default.
• W2591610528 hasConcept C55493867 @default.
• W2591610528 hasConcept C60644358 @default.
• W2591610528 hasConcept C86803240 @default.
• W2591610528 hasConcept C89423630 @default.
• W2591610528 hasConcept C90583042 @default.
• W2591610528 hasConceptScore W2591610528C104317684 @default.
• W2591610528 hasConceptScore W2591610528C159047783 @default.
• W2591610528 hasConceptScore W2591610528C2522874641 @default.
• W2591610528 hasConceptScore W2591610528C2777391075 @default.
• W2591610528 hasConceptScore W2591610528C2781188995 @default.
• W2591610528 hasConceptScore W2591610528C2781196997 @default.
• W2591610528 hasConceptScore W2591610528C49105822 @default.
• W2591610528 hasConceptScore W2591610528C55493867 @default.
• W2591610528 hasConceptScore W2591610528C60644358 @default.
• W2591610528 hasConceptScore W2591610528C86803240 @default.
• W2591610528 hasConceptScore W2591610528C89423630 @default.
• W2591610528 hasConceptScore W2591610528C90583042 @default.
• W2591610528 hasIssue "3" @default.
• W2591610528 hasLocation W25916105281 @default.
• W2591610528 hasLocation W25916105282 @default.
• W2591610528 hasOpenAccess W2591610528 @default.
• W2591610528 hasPrimaryLocation W25916105281 @default.
• W2591610528 hasRelatedWork W1972800817 @default.
• W2591610528 hasRelatedWork W1984842047 @default.
• W2591610528 hasRelatedWork W2016052042 @default.
• W2591610528 hasRelatedWork W2158340333 @default.
• W2591610528 hasRelatedWork W2302132233 @default.
• W2591610528 hasRelatedWork W2319498260 @default.
• W2591610528 hasRelatedWork W2353812755 @default.
• W2591610528 hasRelatedWork W2557491197 @default.
• W2591610528 hasRelatedWork W3140247098 @default.
• W2591610528 hasRelatedWork W4245855095 @default.
• W2591610528 hasVolume "58" @default.
• W2591610528 isParatext "false" @default.

• next