Matches in SemOpenAlex for { <https://semopenalex.org/work/W2591747248> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2591747248 abstract "Single-molecule recycling (SMR) in a nanochannel, in which a molecule in solution quickly passes through a focused laser beam and the solution flow is reversed after a set delay following each passage, provides an attractive alternative to feedback-driven trapping for prolonging the observation of a single molecule in a confocal microscope, as most of the time the molecule is in the dark, which extends the time before irreversible photobleaching and also gives time for recovery from photogenerated reversible dark states between passages. Guided by suggestions in previous SMR reports, we have utilized a National Instruments FPGA card and LabVIEW Realtime to implement 10 ns photon time-stamping, weighted sliding sum digital filtering, maximum-likelihood (ML) analysis of photon time-stamps, and real-time control of electrokinetic voltage in SMR experiments in order to improve the detection and timing of passages of the single molecule through the focused laser spot. We have developed a ML technique for measuring the diffusivity of the single molecule in the nanochannel, which uses a look-up table to update the probability density function of the diffusivity with each detected passage, thereby also providing confidence limits for the measurement. We use Monte Carlo simulations to examine prior experiments, validate the ML diffusivity measurement strategy, and evaluate choice of experimental parameters." @default.
- W2591747248 created "2017-03-16" @default.
- W2591747248 creator A5012183092 @default.
- W2591747248 creator A5056168495 @default.
- W2591747248 date "2017-02-21" @default.
- W2591747248 modified "2023-09-25" @default.
- W2591747248 title "Improved timing and diffusivity measurement in single-molecule recycling in a nanochannel" @default.
- W2591747248 cites W1976799229 @default.
- W2591747248 cites W2029069256 @default.
- W2591747248 cites W2035368727 @default.
- W2591747248 cites W2061391624 @default.
- W2591747248 cites W2113175661 @default.
- W2591747248 cites W2133701212 @default.
- W2591747248 cites W2152248309 @default.
- W2591747248 cites W2158698604 @default.
- W2591747248 doi "https://doi.org/10.1117/12.2252555" @default.
- W2591747248 hasPublicationYear "2017" @default.
- W2591747248 type Work @default.
- W2591747248 sameAs 2591747248 @default.
- W2591747248 citedByCount "1" @default.
- W2591747248 countsByYear W25917472482021 @default.
- W2591747248 crossrefType "proceedings-article" @default.
- W2591747248 hasAuthorship W2591747248A5012183092 @default.
- W2591747248 hasAuthorship W2591747248A5056168495 @default.
- W2591747248 hasConcept C105795698 @default.
- W2591747248 hasConcept C120665830 @default.
- W2591747248 hasConcept C121332964 @default.
- W2591747248 hasConcept C136009344 @default.
- W2591747248 hasConcept C159317903 @default.
- W2591747248 hasConcept C192562407 @default.
- W2591747248 hasConcept C197055811 @default.
- W2591747248 hasConcept C33923547 @default.
- W2591747248 hasConcept C37668627 @default.
- W2591747248 hasConcept C41008148 @default.
- W2591747248 hasConcept C520434653 @default.
- W2591747248 hasConcept C62520636 @default.
- W2591747248 hasConcept C67649825 @default.
- W2591747248 hasConceptScore W2591747248C105795698 @default.
- W2591747248 hasConceptScore W2591747248C120665830 @default.
- W2591747248 hasConceptScore W2591747248C121332964 @default.
- W2591747248 hasConceptScore W2591747248C136009344 @default.
- W2591747248 hasConceptScore W2591747248C159317903 @default.
- W2591747248 hasConceptScore W2591747248C192562407 @default.
- W2591747248 hasConceptScore W2591747248C197055811 @default.
- W2591747248 hasConceptScore W2591747248C33923547 @default.
- W2591747248 hasConceptScore W2591747248C37668627 @default.
- W2591747248 hasConceptScore W2591747248C41008148 @default.
- W2591747248 hasConceptScore W2591747248C520434653 @default.
- W2591747248 hasConceptScore W2591747248C62520636 @default.
- W2591747248 hasConceptScore W2591747248C67649825 @default.
- W2591747248 hasLocation W25917472481 @default.
- W2591747248 hasOpenAccess W2591747248 @default.
- W2591747248 hasPrimaryLocation W25917472481 @default.
- W2591747248 hasRelatedWork W1533201884 @default.
- W2591747248 hasRelatedWork W1970054703 @default.
- W2591747248 hasRelatedWork W1979227821 @default.
- W2591747248 hasRelatedWork W1982899134 @default.
- W2591747248 hasRelatedWork W1986728867 @default.
- W2591747248 hasRelatedWork W2032814381 @default.
- W2591747248 hasRelatedWork W2033028181 @default.
- W2591747248 hasRelatedWork W2038850709 @default.
- W2591747248 hasRelatedWork W2056653332 @default.
- W2591747248 hasRelatedWork W2070171776 @default.
- W2591747248 hasRelatedWork W2071293932 @default.
- W2591747248 hasRelatedWork W2528525486 @default.
- W2591747248 hasRelatedWork W2619346018 @default.
- W2591747248 hasRelatedWork W3098831175 @default.
- W2591747248 hasRelatedWork W3127892618 @default.
- W2591747248 hasRelatedWork W3160199327 @default.
- W2591747248 hasRelatedWork W820465735 @default.
- W2591747248 hasRelatedWork W1969220360 @default.
- W2591747248 hasRelatedWork W2964888746 @default.
- W2591747248 hasRelatedWork W3020198788 @default.
- W2591747248 isParatext "false" @default.
- W2591747248 isRetracted "false" @default.
- W2591747248 magId "2591747248" @default.
- W2591747248 workType "article" @default.