Matches in SemOpenAlex for { <https://semopenalex.org/work/W2591980198> ?p ?o ?g. }
- W2591980198 endingPage "521" @default.
- W2591980198 startingPage "511" @default.
- W2591980198 abstract "Recently, with the dramatic increase in demand for therapeutic antibodies, Chinese hamster ovary (CHO) cell culture systems have made significant progress in recombinant antibody production. Over the past two decades, recombinant antibody productivity has been improved by more than 100-fold. Medium optimization has been identified as an important key approach for increasing product concentrations. In this study, we evaluated the effects of deoxyuridine addition to fed-batch cultures of antibody-expressing CHO cell lines. Furthermore, we investigated the effects of combined addition of deoxyuridine, thymidine, and deoxycytidine. Our results suggest that addition of these pyrimidine nucleosides can increase CHO cell growth, with no significant change in the specific production rate. As a result of the increased cell growth, the antibody concentration was elevated and we were able to achieve more than 9 g/L during 16 days of culture. Similar effects of nucleoside addition were observed in fed-batch cultures of a Fab fragment-expressing CHO cell line, and the final Fab fragment concentration was more than 4 g/L. This nucleoside addition strategy could be a powerful platform for efficient antibody production." @default.
- W2591980198 created "2017-03-16" @default.
- W2591980198 creator A5001629306 @default.
- W2591980198 creator A5002595269 @default.
- W2591980198 creator A5023005896 @default.
- W2591980198 creator A5061847309 @default.
- W2591980198 date "2017-03-02" @default.
- W2591980198 modified "2023-10-18" @default.
- W2591980198 title "The enhancement of antibody concentration and achievement of high cell density CHO cell cultivation by adding nucleoside" @default.
- W2591980198 cites W1498699116 @default.
- W2591980198 cites W1784586630 @default.
- W2591980198 cites W1973762921 @default.
- W2591980198 cites W1974459715 @default.
- W2591980198 cites W1985727066 @default.
- W2591980198 cites W1986406786 @default.
- W2591980198 cites W2000213886 @default.
- W2591980198 cites W2006636970 @default.
- W2591980198 cites W2006994535 @default.
- W2591980198 cites W2016490955 @default.
- W2591980198 cites W2020455821 @default.
- W2591980198 cites W2024074032 @default.
- W2591980198 cites W2025119470 @default.
- W2591980198 cites W2029878671 @default.
- W2591980198 cites W2045074917 @default.
- W2591980198 cites W2046105552 @default.
- W2591980198 cites W2053454347 @default.
- W2591980198 cites W2060385448 @default.
- W2591980198 cites W2063700718 @default.
- W2591980198 cites W2066318808 @default.
- W2591980198 cites W2071297165 @default.
- W2591980198 cites W2071793771 @default.
- W2591980198 cites W2079933818 @default.
- W2591980198 cites W2085856131 @default.
- W2591980198 cites W2095522936 @default.
- W2591980198 cites W2109666318 @default.
- W2591980198 cites W2117045376 @default.
- W2591980198 cites W2117471612 @default.
- W2591980198 cites W2118118130 @default.
- W2591980198 cites W2118540273 @default.
- W2591980198 cites W2128761324 @default.
- W2591980198 cites W2152015546 @default.
- W2591980198 cites W2156639989 @default.
- W2591980198 cites W2157181633 @default.
- W2591980198 cites W2160724997 @default.
- W2591980198 cites W2161263785 @default.
- W2591980198 cites W2161810778 @default.
- W2591980198 cites W2162406947 @default.
- W2591980198 cites W2168338758 @default.
- W2591980198 cites W2171701744 @default.
- W2591980198 cites W2289965054 @default.
- W2591980198 cites W2292054199 @default.
- W2591980198 cites W2408938989 @default.
- W2591980198 cites W2917973694 @default.
- W2591980198 cites W4233249586 @default.
- W2591980198 cites W4365787623 @default.
- W2591980198 doi "https://doi.org/10.1007/s10616-017-0066-7" @default.
- W2591980198 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5461242" @default.
- W2591980198 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28251404" @default.
- W2591980198 hasPublicationYear "2017" @default.
- W2591980198 type Work @default.
- W2591980198 sameAs 2591980198 @default.
- W2591980198 citedByCount "33" @default.
- W2591980198 countsByYear W25919801982017 @default.
- W2591980198 countsByYear W25919801982018 @default.
- W2591980198 countsByYear W25919801982019 @default.
- W2591980198 countsByYear W25919801982020 @default.
- W2591980198 countsByYear W25919801982021 @default.
- W2591980198 countsByYear W25919801982022 @default.
- W2591980198 countsByYear W25919801982023 @default.
- W2591980198 crossrefType "journal-article" @default.
- W2591980198 hasAuthorship W2591980198A5001629306 @default.
- W2591980198 hasAuthorship W2591980198A5002595269 @default.
- W2591980198 hasAuthorship W2591980198A5023005896 @default.
- W2591980198 hasAuthorship W2591980198A5061847309 @default.
- W2591980198 hasBestOaLocation W25919801982 @default.
- W2591980198 hasConcept C104317684 @default.
- W2591980198 hasConcept C153911025 @default.
- W2591980198 hasConcept C159654299 @default.
- W2591980198 hasConcept C175656101 @default.
- W2591980198 hasConcept C185592680 @default.
- W2591980198 hasConcept C203014093 @default.
- W2591980198 hasConcept C2776543447 @default.
- W2591980198 hasConcept C2777108182 @default.
- W2591980198 hasConcept C2777111502 @default.
- W2591980198 hasConcept C40767141 @default.
- W2591980198 hasConcept C54355233 @default.
- W2591980198 hasConcept C552990157 @default.
- W2591980198 hasConcept C55493867 @default.
- W2591980198 hasConcept C62112901 @default.
- W2591980198 hasConcept C81885089 @default.
- W2591980198 hasConcept C86803240 @default.
- W2591980198 hasConceptScore W2591980198C104317684 @default.
- W2591980198 hasConceptScore W2591980198C153911025 @default.
- W2591980198 hasConceptScore W2591980198C159654299 @default.
- W2591980198 hasConceptScore W2591980198C175656101 @default.
- W2591980198 hasConceptScore W2591980198C185592680 @default.
- W2591980198 hasConceptScore W2591980198C203014093 @default.
- W2591980198 hasConceptScore W2591980198C2776543447 @default.