FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2592736407> ?p ?o ?g. }

• W2592736407 endingPage "351" @default.
• W2592736407 startingPage "351" @default.
• W2592736407 abstract "The lactocrine hypothesis describes a mechanism whereby milk-borne bioactive factors are delivered to offspring via nursing and affect development of neonatal tissues. In support of this hypothesis, data for the uterus indicate that, in comparison with gilts fed a porcine milk replacer for two days from birth (postnatal day = PND 0), nursing is essential for support of normal uterine estrogen receptor-α (ESR1), vascular endothelial growth factor (VEGF) A, and matrix metalloproteinase (MMP) 9 protein expression at PND 2. Depressed expression of these markers and mediators of uterine growth and remodeling was not restored when gilts fed milk replacer through PND 2 were switched to nursing until PND 14. These results suggest that a window of opportunity for lactocrine signaling exists within the first two days of life; however, the critical time period for such signaling within the first 48 h from birth is unknown. Here, objectives were to determine the effects of: (1) age at first nursing (0 h, 30 min, 12 h); and (2) duration of nursing (30 min, 12 h, 48 h) on uterine ESR1, VEGFA and MMP9 expression. Gilts (n=5-6/group) were randomly assigned at birth to one of six treatment groups: (1) nursed for 30 min from birth and gavage-fed milk replacer (30ml/kg BW/2 h) for 47.5 h; (2) nursed for 12 h from birth and gavage-fed milk replacer for 36 h; (3) nursed ad libitum for 48 h; (4) gavage-fed milk replacer for 30 min from birth and nursed for 47.5 h; (5) gavage-fed milk replacer for 12 h from birth and nursed for 36 h; or (6) gavage-fed milk replacer for 48 h. Uteri were collected on PND 2 (50 h). ESR1, VEGFA, and MMP9 protein expression was evaluated by immunoblotting using actin as a loading control and quantified by densitometry. Immunoreactive bands at 51 kDa for ESR1, 46 kDa for VEGFA, and 84 and 92 kDa for MMP 9 and pro-MMP9 were detected consistently in uterine tissues from gilts allowed to nurse beginning at 0 h or 30 min of age. However, as observed in gilts fed replacer for 48 h from birth, targeted proteins were undetectable in uterine tissues on PND 2 when gilts were nursed starting at 12 h after birth. Nursing for as little as 30 min from birth was sufficient to induce uterine ESR1, VEGFA and MMP9 expression at PND 2. Increasing the duration of nursing from 30 min to 12 h increased expression of targeted proteins to levels similar to those seen in gilts nursed for 48 h. Results indicate that age at first nursing is important for lactocrine signaling. Nursing initiated at 0 h or 30 min of age is sufficient to induce a normal developmental program as reflected by uterine ESR1, VEGFA, and MMP9 expression on PND 2. However, when a lactocrine-null period is imposed from birth to 12 h of age, resumption of nursing through PND 2 failed to rescue the normal uterine developmental program. Thus, the window for lactocrine signaling important for neonatal uterine development is open within 12 h of birth. Results also show that increased nursing duration correlates positively with levels of uterine ESR1, VEGFA, and MMP9 expression. This study illustrates the importance of age at first nursing and duration of nursing in support of the porcine uterine developmental program and defines the critical period for lactocrine signaling more precisely within two days of birth. [Support: USDA-NRICGP 2007-35203-18098 and NSF-EPS-0814103] (poster)" @default.
• W2592736407 created "2017-03-16" @default.
• W2592736407 creator A5002619546 @default.
• W2592736407 creator A5015255932 @default.
• W2592736407 creator A5016526372 @default.
• W2592736407 creator A5023479477 @default.
• W2592736407 creator A5071806211 @default.
• W2592736407 creator A5072473233 @default.
• W2592736407 date "2011-07-01" @default.
• W2592736407 modified "2023-09-25" @default.
• W2592736407 title "Age and Duration-Dependent Effects of Lactocrine Signaling on Markers and Mediators of Uterine Growth and Remodeling in Neonatal Gilts." @default.
• W2592736407 doi "https://doi.org/10.1093/biolreprod/85.s1.351" @default.
• W2592736407 hasPublicationYear "2011" @default.
• W2592736407 type Work @default.
• W2592736407 sameAs 2592736407 @default.
• W2592736407 citedByCount "0" @default.
• W2592736407 crossrefType "journal-article" @default.
• W2592736407 hasAuthorship W2592736407A5002619546 @default.
• W2592736407 hasAuthorship W2592736407A5015255932 @default.
• W2592736407 hasAuthorship W2592736407A5016526372 @default.
• W2592736407 hasAuthorship W2592736407A5023479477 @default.
• W2592736407 hasAuthorship W2592736407A5071806211 @default.
• W2592736407 hasAuthorship W2592736407A5072473233 @default.
• W2592736407 hasConcept C109523444 @default.
• W2592736407 hasConcept C112672928 @default.
• W2592736407 hasConcept C121608353 @default.
• W2592736407 hasConcept C126322002 @default.
• W2592736407 hasConcept C134018914 @default.
• W2592736407 hasConcept C159110408 @default.
• W2592736407 hasConcept C16685009 @default.
• W2592736407 hasConcept C170493617 @default.
• W2592736407 hasConcept C2776659692 @default.
• W2592736407 hasConcept C2777164284 @default.
• W2592736407 hasConcept C2779066055 @default.
• W2592736407 hasConcept C2779234561 @default.
• W2592736407 hasConcept C530470458 @default.
• W2592736407 hasConcept C54355233 @default.
• W2592736407 hasConcept C71924100 @default.
• W2592736407 hasConcept C84606932 @default.
• W2592736407 hasConcept C86803240 @default.
• W2592736407 hasConceptScore W2592736407C109523444 @default.
• W2592736407 hasConceptScore W2592736407C112672928 @default.
• W2592736407 hasConceptScore W2592736407C121608353 @default.
• W2592736407 hasConceptScore W2592736407C126322002 @default.
• W2592736407 hasConceptScore W2592736407C134018914 @default.
• W2592736407 hasConceptScore W2592736407C159110408 @default.
• W2592736407 hasConceptScore W2592736407C16685009 @default.
• W2592736407 hasConceptScore W2592736407C170493617 @default.
• W2592736407 hasConceptScore W2592736407C2776659692 @default.
• W2592736407 hasConceptScore W2592736407C2777164284 @default.
• W2592736407 hasConceptScore W2592736407C2779066055 @default.
• W2592736407 hasConceptScore W2592736407C2779234561 @default.
• W2592736407 hasConceptScore W2592736407C530470458 @default.
• W2592736407 hasConceptScore W2592736407C54355233 @default.
• W2592736407 hasConceptScore W2592736407C71924100 @default.
• W2592736407 hasConceptScore W2592736407C84606932 @default.
• W2592736407 hasConceptScore W2592736407C86803240 @default.
• W2592736407 hasIssue "Suppl_1" @default.
• W2592736407 hasLocation W25927364071 @default.
• W2592736407 hasOpenAccess W2592736407 @default.
• W2592736407 hasPrimaryLocation W25927364071 @default.
• W2592736407 hasRelatedWork W2004424018 @default.
• W2592736407 hasRelatedWork W2068408721 @default.
• W2592736407 hasRelatedWork W2076203191 @default.
• W2592736407 hasRelatedWork W2077978869 @default.
• W2592736407 hasRelatedWork W2158864732 @default.
• W2592736407 hasRelatedWork W2162614805 @default.
• W2592736407 hasRelatedWork W2330971285 @default.
• W2592736407 hasRelatedWork W2334410700 @default.
• W2592736407 hasRelatedWork W2544107670 @default.
• W2592736407 hasRelatedWork W2740214960 @default.
• W2592736407 hasVolume "85" @default.
• W2592736407 isParatext "false" @default.
• W2592736407 isRetracted "false" @default.
• W2592736407 magId "2592736407" @default.
• W2592736407 workType "article" @default.