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- W2592843175 abstract "Alibrary ofthePsesdomonas putda chromosome, prepared through theuseofthecosmid pJB8ligated to apartial Sau3Adigest ofbacterial DNA,followed byinvitro pa ing intobacteriophage lambdaparticles, wasusedtoconstruct astrain ofEscherichia coliwhichcontained thegenesforhistidine utilization. Thisisolate produced a repressorproduct andallfive enzymesrequired inPseudomonas spp.forhistidine dissimilation, whereas noneofthese could bedetected inthenontransduced parent E.coli strain. Whenthis transductant was grown on various mediacontaining histidine orurocanate astheinducer, itwas observed thatproduction of thecloned histidine clegradative enzymeswas influenced somewhat bythechoice ofnitrogen sourceusedbut notbythecarbon source.Therecombinant cosmid was isolated andfound toconsist of21.1kiolbase pairsof DNA,withapproximately 16kilobase pairs derived fromPseudomonas DNAandtheremainder being fromthe pJB8 vector. Digestion ofthisinsert DNA withEcoRIprovided a 6.1-kilobase-pair fragment which, upon ligation inpUC8andtransformation into anE.coil host, was found toencode histidine ammonia-lyase and urocanase.Theinducible nature ofthis production indicated that thehutrepressor genealso was present on this fragment. Insertional inactivation ofthehistidine ammonia-lyase andurocanase genesbythegamma-delta transposon haspermitted location ofthese structural genesandhasprovided evidence thattranscription proceeds fromurocanasethrough histidine ammonia-lyase. Mapping ofthe16-kilobase-pair Pseudomonas DNAsegment withrestriction enzymesandsubcloning ofadditional portions, oneofwhichcontained thegene forformiminoglutamate hydrolase andanother that could constitutively expressactivities forbothimidazolone propionate hydrolase andformylglutamate hydrolase, hasprovided evidence fortheorganization ofallhut genes." @default.
- W2592843175 created "2017-03-16" @default.
- W2592843175 creator A5052085912 @default.
- W2592843175 date "1985-01-01" @default.
- W2592843175 modified "2023-09-24" @default.
- W2592843175 title "Cloning andExpression inEscherichia coli ofHistidine Utilization GenesfromPseudomonas putidat" @default.
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