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- W2593032885 abstract "we devised an assay for validation and measurements of nitrites (NO 2 ) and nitrates (NO 3 ) by liquid chromatography with UV detection. It was made in homogenate brain of rats treated with pentylenetetrazole (PTZ) 20 mg/kg and NaCl 0.9 %. Assay linearity for standards ranged between 10-100 nM with correlation coefficient (r) of 0.9999. The limit of detection for NO 2 and NO 3 in standards was 1 nM. Determination coefficient (r) 0.9998. The slope of the regression line was different from zero. The analysis of varianza test proved no showed significant differences between slopes in standards. The method was accurate and was satisfactory with a coefficient of variation less than 15 %. The method turned out to be rapid, selective, linear, reproducible and accurate. Our procedure may be reliable for determination of NO 2 and NO 3 in rat brain. Palabras clave: Cerebro, PTZ, HPLC, nitrito, nitrato" @default.
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- W2593032885 date "2005-01-01" @default.
- W2593032885 modified "2023-09-27" @default.
- W2593032885 title "Nuevo método de cromatografía de líquidos para medición de nitritos y nitratos en cerebro de rata New method for measure nitrites and nitrates levels in rat brain by liquid chromatography" @default.
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