FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2593113312> ?p ?o ?g. }

• W2593113312 abstract "Drugs often kill some cancer cells while others survive. This stochastic outcome is seen even in clonal cells grown under the same conditions. Understanding the molecular reasons for this stochastic outcome is a current challenge, which requires studying the proteome at the single cell level over time. In a previous study we used dynamic proteomics to study the response of cancer cells to a DNA damaging drug, camptothecin. Several proteins showed bimodal dynamics: they rose in some cells and decreased in others, in a way that correlated with eventual cell fate: death or survival. Here we ask whether bimodality is a special case for camptothecin, or whether it occurs for other drugs as well. To address this, we tested a second drug with a different mechanism of action, an HSP90 inhibitor. We used dynamic proteomics to follow 100 proteins in space and time, endogenously tagged in their native chromosomal location in individual living human lung-cancer cells, following drug administration.We find bimodal dynamics for a quarter of the proteins. In some cells these proteins strongly rise in level about 12 h after treatment, but in other cells their level drops or remains constant. The proteins which rise in surviving cells included anti-apoptotic factors such as DDX5, and cell cycle regulators such as RFC1. The proteins that rise in cells that eventually die include pro-apoptotic factors such as APAF1. The two drugs shared some aspects in their single-cell response, including 7 of the bimodal proteins and translocation of oxidative response proteins to the nucleus, but differed in other aspects, with HSP90i showing more bimodal proteins. Moreover, the cell cycle phase at drug administration impacted the probability to die from HSP90i but not camptothecin.Single-cell dynamic proteomics reveals sub-populations of cells within a clonal cell line with different protein dynamics in response to a drug. These different dynamics correlate with cell survival or death. Bimodal proteins which correlate with cell fate may be potential drug targets to enhance the effects of therapy." @default.
• W2593113312 created "2017-03-16" @default.
• W2593113312 creator A5010209966 @default.
• W2593113312 creator A5017206938 @default.
• W2593113312 creator A5028437651 @default.
• W2593113312 creator A5054622481 @default.
• W2593113312 date "2017-03-07" @default.
• W2593113312 modified "2023-10-12" @default.
• W2593113312 title "Dynamic proteomics reveals bimodal protein dynamics of cancer cells in response to HSP90 inhibitor" @default.
• W2593113312 cites W1536538430 @default.
• W2593113312 cites W1692324497 @default.
• W2593113312 cites W1974934611 @default.
• W2593113312 cites W1987012261 @default.
• W2593113312 cites W1997626871 @default.
• W2593113312 cites W1998728654 @default.
• W2593113312 cites W2002851265 @default.
• W2593113312 cites W2006105383 @default.
• W2593113312 cites W2006818294 @default.
• W2593113312 cites W2009980816 @default.
• W2593113312 cites W2010601590 @default.
• W2593113312 cites W2013324041 @default.
• W2593113312 cites W2015213533 @default.
• W2593113312 cites W2018607339 @default.
• W2593113312 cites W2022531767 @default.
• W2593113312 cites W2029533042 @default.
• W2593113312 cites W2050296416 @default.
• W2593113312 cites W2061265815 @default.
• W2593113312 cites W2062880004 @default.
• W2593113312 cites W2063926640 @default.
• W2593113312 cites W2077244922 @default.
• W2593113312 cites W2087684358 @default.
• W2593113312 cites W2106391833 @default.
• W2593113312 cites W2111510713 @default.
• W2593113312 cites W2115995437 @default.
• W2593113312 cites W2124426078 @default.
• W2593113312 cites W2129297473 @default.
• W2593113312 cites W2135461398 @default.
• W2593113312 cites W2138784667 @default.
• W2593113312 cites W2152627545 @default.
• W2593113312 cites W2153574940 @default.
• W2593113312 cites W2332292689 @default.
• W2593113312 cites W4245469960 @default.
• W2593113312 doi "https://doi.org/10.1186/s12918-017-0410-8" @default.
• W2593113312 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5341406" @default.
• W2593113312 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28270142" @default.
• W2593113312 hasPublicationYear "2017" @default.
• W2593113312 type Work @default.
• W2593113312 sameAs 2593113312 @default.
• W2593113312 citedByCount "11" @default.
• W2593113312 countsByYear W25931133122017 @default.
• W2593113312 countsByYear W25931133122018 @default.
• W2593113312 countsByYear W25931133122021 @default.
• W2593113312 countsByYear W25931133122022 @default.
• W2593113312 crossrefType "journal-article" @default.
• W2593113312 hasAuthorship W2593113312A5010209966 @default.
• W2593113312 hasAuthorship W2593113312A5017206938 @default.
• W2593113312 hasAuthorship W2593113312A5028437651 @default.
• W2593113312 hasAuthorship W2593113312A5054622481 @default.
• W2593113312 hasBestOaLocation W25931133121 @default.
• W2593113312 hasConcept C104317684 @default.
• W2593113312 hasConcept C104397665 @default.
• W2593113312 hasConcept C121608353 @default.
• W2593113312 hasConcept C143425029 @default.
• W2593113312 hasConcept C1491633281 @default.
• W2593113312 hasConcept C190283241 @default.
• W2593113312 hasConcept C2779682216 @default.
• W2593113312 hasConcept C29537977 @default.
• W2593113312 hasConcept C46111723 @default.
• W2593113312 hasConcept C502942594 @default.
• W2593113312 hasConcept C54355233 @default.
• W2593113312 hasConcept C552990157 @default.
• W2593113312 hasConcept C55493867 @default.
• W2593113312 hasConcept C60644358 @default.
• W2593113312 hasConcept C80311884 @default.
• W2593113312 hasConcept C86803240 @default.
• W2593113312 hasConcept C95444343 @default.
• W2593113312 hasConcept C96232424 @default.
• W2593113312 hasConceptScore W2593113312C104317684 @default.
• W2593113312 hasConceptScore W2593113312C104397665 @default.
• W2593113312 hasConceptScore W2593113312C121608353 @default.
• W2593113312 hasConceptScore W2593113312C143425029 @default.
• W2593113312 hasConceptScore W2593113312C1491633281 @default.
• W2593113312 hasConceptScore W2593113312C190283241 @default.
• W2593113312 hasConceptScore W2593113312C2779682216 @default.
• W2593113312 hasConceptScore W2593113312C29537977 @default.
• W2593113312 hasConceptScore W2593113312C46111723 @default.
• W2593113312 hasConceptScore W2593113312C502942594 @default.
• W2593113312 hasConceptScore W2593113312C54355233 @default.
• W2593113312 hasConceptScore W2593113312C552990157 @default.
• W2593113312 hasConceptScore W2593113312C55493867 @default.
• W2593113312 hasConceptScore W2593113312C60644358 @default.
• W2593113312 hasConceptScore W2593113312C80311884 @default.
• W2593113312 hasConceptScore W2593113312C86803240 @default.
• W2593113312 hasConceptScore W2593113312C95444343 @default.
• W2593113312 hasConceptScore W2593113312C96232424 @default.
• W2593113312 hasFunder F4320322252 @default.
• W2593113312 hasIssue "1" @default.
• W2593113312 hasLocation W25931133121 @default.
• W2593113312 hasLocation W25931133122 @default.
• W2593113312 hasLocation W25931133123 @default.

• next