FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2593138384> ?p ?o ?g. }

• W2593138384 abstract "Vertebrate eyes are known to contain circadian clocks, however, the intracellular mechanisms regulating the retinal clockwork remain largely unknown. To address this, we generated a cell line (hRPE-YC) from human retinal pigmental epithelium, which stably co-expressed reporters for molecular clock oscillations (Bmal1-luciferase) and intracellular Ca2+ concentrations (YC3.6). The hRPE-YC cells demonstrated circadian rhythms in Bmal1 transcription. Also, these cells represented circadian rhythms in Ca2+-spiking frequencies, which were canceled by dominant-negative Bmal1 transfections. The muscarinic agonist carbachol, but not photic stimulation, phase-shifted Bmal1 transcriptional rhythms with a type-1 phase response curve. This is consistent with significant M3 muscarinic receptor expression and little photo-sensor (Cry2 and Opn4) expression in these cells. Moreover, forskolin phase-shifted Bmal1 transcriptional rhythm with a type-0 phase response curve, in accordance with long-lasting CREB phosphorylation levels after forskolin exposure. Interestingly, the hRPE-YC cells demonstrated apparent circadian rhythms in phagocytic activities, which were abolished by carbachol or dominant-negative Bmal1 transfection. Because phagocytosis in RPE cells determines photoreceptor disc shedding, molecular clock oscillations and cytosolic Ca2+ signaling may be the driving forces for disc-shedding rhythms known in various vertebrates. In conclusion, the present study provides a cellular model to understand molecular and intracellular signaling mechanisms underlying human retinal circadian clocks." @default.
• W2593138384 created "2017-03-16" @default.
• W2593138384 creator A5005075481 @default.
• W2593138384 creator A5010210218 @default.
• W2593138384 creator A5014444085 @default.
• W2593138384 creator A5018667058 @default.
• W2593138384 creator A5027434950 @default.
• W2593138384 creator A5028592309 @default.
• W2593138384 creator A5053975249 @default.
• W2593138384 creator A5060102784 @default.
• W2593138384 creator A5072591607 @default.
• W2593138384 creator A5076757204 @default.
• W2593138384 date "2017-03-09" @default.
• W2593138384 modified "2023-09-23" @default.
• W2593138384 title "Regulation of molecular clock oscillations and phagocytic activity via muscarinic Ca2+ signaling in human retinal pigment epithelial cells" @default.
• W2593138384 cites W118255430 @default.
• W2593138384 cites W1546846595 @default.
• W2593138384 cites W1674901911 @default.
• W2593138384 cites W1963591622 @default.
• W2593138384 cites W1966595619 @default.
• W2593138384 cites W1971013361 @default.
• W2593138384 cites W1972651922 @default.
• W2593138384 cites W1973622551 @default.
• W2593138384 cites W1976263073 @default.
• W2593138384 cites W1985345337 @default.
• W2593138384 cites W1986517122 @default.
• W2593138384 cites W1986608768 @default.
• W2593138384 cites W1987319020 @default.
• W2593138384 cites W1987638303 @default.
• W2593138384 cites W1988214506 @default.
• W2593138384 cites W1998448534 @default.
• W2593138384 cites W2002297937 @default.
• W2593138384 cites W2003184463 @default.
• W2593138384 cites W2003302378 @default.
• W2593138384 cites W2007395018 @default.
• W2593138384 cites W2018427418 @default.
• W2593138384 cites W2018475001 @default.
• W2593138384 cites W2021199664 @default.
• W2593138384 cites W2025255307 @default.
• W2593138384 cites W2028221037 @default.
• W2593138384 cites W2029323550 @default.
• W2593138384 cites W2029517170 @default.
• W2593138384 cites W2029646077 @default.
• W2593138384 cites W2030084446 @default.
• W2593138384 cites W2036458630 @default.
• W2593138384 cites W2041362984 @default.
• W2593138384 cites W2042227355 @default.
• W2593138384 cites W2042653212 @default.
• W2593138384 cites W2048485183 @default.
• W2593138384 cites W2051747007 @default.
• W2593138384 cites W2051975450 @default.
• W2593138384 cites W2055969703 @default.
• W2593138384 cites W2078689239 @default.
• W2593138384 cites W2079230059 @default.
• W2593138384 cites W2080428496 @default.
• W2593138384 cites W2084292395 @default.
• W2593138384 cites W2086414488 @default.
• W2593138384 cites W2089559975 @default.
• W2593138384 cites W2101746016 @default.
• W2593138384 cites W2104809074 @default.
• W2593138384 cites W2108761772 @default.
• W2593138384 cites W2112214463 @default.
• W2593138384 cites W2114930529 @default.
• W2593138384 cites W2128878853 @default.
• W2593138384 cites W2129022768 @default.
• W2593138384 cites W2130496406 @default.
• W2593138384 cites W2130866419 @default.
• W2593138384 cites W2131552046 @default.
• W2593138384 cites W2132712869 @default.
• W2593138384 cites W2144454171 @default.
• W2593138384 cites W2146843966 @default.
• W2593138384 cites W2148756146 @default.
• W2593138384 cites W2153041000 @default.
• W2593138384 cites W2153312133 @default.
• W2593138384 cites W2160550697 @default.
• W2593138384 cites W2303447433 @default.
• W2593138384 doi "https://doi.org/10.1038/srep44175" @default.
• W2593138384 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5343479" @default.
• W2593138384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28276525" @default.
• W2593138384 hasPublicationYear "2017" @default.
• W2593138384 type Work @default.
• W2593138384 sameAs 2593138384 @default.
• W2593138384 citedByCount "17" @default.
• W2593138384 countsByYear W25931383842018 @default.
• W2593138384 countsByYear W25931383842019 @default.
• W2593138384 countsByYear W25931383842020 @default.
• W2593138384 countsByYear W25931383842021 @default.
• W2593138384 countsByYear W25931383842022 @default.
• W2593138384 countsByYear W25931383842023 @default.
• W2593138384 crossrefType "journal-article" @default.
• W2593138384 hasAuthorship W2593138384A5005075481 @default.
• W2593138384 hasAuthorship W2593138384A5010210218 @default.
• W2593138384 hasAuthorship W2593138384A5014444085 @default.
• W2593138384 hasAuthorship W2593138384A5018667058 @default.
• W2593138384 hasAuthorship W2593138384A5027434950 @default.
• W2593138384 hasAuthorship W2593138384A5028592309 @default.
• W2593138384 hasAuthorship W2593138384A5053975249 @default.
• W2593138384 hasAuthorship W2593138384A5060102784 @default.
• W2593138384 hasAuthorship W2593138384A5072591607 @default.
• W2593138384 hasAuthorship W2593138384A5076757204 @default.

• next