Matches in SemOpenAlex for { <https://semopenalex.org/work/W2593147717> ?p ?o ?g. }
- W2593147717 abstract "Highly purified human thrombin stimulates the adherence of polymorphonuclear leukocytes (PMNs) to vascular endothelial cells (EC). When Indium-labeled PMNs were incubated with primary monolayers of cultured human umbilical vein EC, the basal adherence was 10±1% of the PMNs at 5 min. Addition of thrombin (2 U/ml) increased the mean adherence to 42±15%. Enhanced neutrophil adherence in response to thrombin was confirmed by experiments with unlabeled leukocytes, examined by phase contrast and scanning electron microscopy. The action of thrombin was on the EC, since it did not directly stimulate PMN adhesiveness when measured by aggregation or by adherence to nylon fiber columns. Furthermore, enhanced neutrophil adherence occurred when endothelial monolayers were treated with thrombin and washed before adding 'Indium ('In)-labeled PMNs. Thrombin that had been inactivated with antithrombin III and heparin did not enhance neutrophil adherence. Prothrombin, Factor Xa, and fibrinogen were also ineffective. The stimulated adherence of PMNs was maximal 5 min after incubation of the EC with thrombin, and decreased thereafter. The response was dose-dependent, with half-maximal stimulation at 0.2-0.25 U thrombin/ml. The enhanced PMN adherence caused by thrombin may result in part from the production of platelet-activating factor (PAF) by the stimulated EC since (a) thrombin-stimulated EC synthesize PAF with a time course and concentration dependence that are similar to the time" @default.
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- W2593147717 date "1985-01-01" @default.
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- W2593147717 title "to Human Endothelial Cells In Vitro" @default.
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