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• W2593174454 abstract "We investigated the slow release of Zn ion (Zn 2+ ) from nanocoatings and compared the in vitro response of osteoblasts (MC3T3-E1) and proosteoclasts (RAW 264.7) cultured on Ca 2 ZnSi 2 O 7 nanocoated with different Zn/Ca molar ratios on a Ti-6Al-4V (i.e., Ti) substrate to optimize cell behaviors and molecule levels. Significant morphology differences were observed among samples. By comparing with pure Ti and CaSiO 3 nanocoating, the morphology of Ca 2 ZnSi 2 O 7 ceramic nanocoatings was rough and contained small nanoparticles or aggregations. Slow Zn 2+ release from nanocoatings was observed and Zn 2+ concentration was regulated by varying the Zn/Ca ratios. The cell-response results showed Ca 2 ZnSi 2 O 7 nanocoating at different Zn/Ca molar ratios for osteoblasts and osteoclasts. Compared to other nanocoatings and Ti, sample Zn/Ca (0.3) showed the highest cell viability and upregulated expression of the osteogenic differentiation genes ALP, COL-1, and OCN. Additionally, sample Zn/Ca (0.3) showed the greatest inhibition of RAW 264.7 cell growth and decreased the mRNA levels of osteoclast-related genes OAR, TRAP, and HYA1. Therefore, the optimal Zn-Ca ratio of 0.3 in Ca 2 ZnSi 2 O 7 ceramic nanocoating on Ti had a dual osteoblast-promoting and osteoclast-inhibiting effect to dynamically balance osteoblasts/osteoclasts. These optimal Zn-Ca ratios are valuable for Ca 2 ZnSi 2 O 7 ceramic nanocoating on Ti-coated implants for potential applications in bone tissue regeneration." @default.
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• W2593174454 date "2017-01-01" @default.
• W2593174454 modified "2023-10-06" @default.
• W2593174454 title "Optimal Zn-Modified Ca–Si-Based Ceramic Nanocoating with Zn Ion Release for Osteoblast Promotion and Osteoclast Inhibition in Bone Tissue Engineering" @default.
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• W2593174454 doi "https://doi.org/10.1155/2017/7374510" @default.
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