Matches in SemOpenAlex for { <https://semopenalex.org/work/W2593189368> ?p ?o ?g. }
- W2593189368 endingPage "e0173747" @default.
- W2593189368 startingPage "e0173747" @default.
- W2593189368 abstract "A growing body of evidence suggests that L-selectin ligands presented on circulating tumor cells facilitate metastasis by binding L-selectin presented on leukocytes. Commonly used methods for detecting L-selectin ligands on tissues, e.g., immunostaining, are performed under static, no-flow conditions. However, such analysis does not assay for functional L-selectin ligands, specifically those ligands that promote adhesion under shear flow conditions. Recently our lab developed a method, termed dynamic biochemical tissue analysis (DBTA), to detect functional selectin ligands in situ by probing tissues with L-selectin-coated microspheres under hemodynamic flow conditions. In this investigation, DBTA was used to probe human colon tissues for L-selectin ligand activity. The detection of L-selectin ligands using DBTA was highly specific. Furthermore, DBTA reproducibly detected functional L-selectin ligands on diseased, e.g., cancerous or inflamed, tissues but not on noncancerous tissues. In addition, DBTA revealed a heterogeneous distribution of functional L-selectin ligands on colon cancer tissues. Most notably, detection of L-selectin ligands by immunostaining using HECA-452 antibody only partially correlated with functional L-selectin ligands detected by DBTA. In summation, the results of this study demonstrate that DBTA detects functional selectin ligands to provide a unique characterization of pathological tissue." @default.
- W2593189368 created "2017-03-16" @default.
- W2593189368 creator A5024015661 @default.
- W2593189368 creator A5026348777 @default.
- W2593189368 creator A5049359415 @default.
- W2593189368 creator A5049540949 @default.
- W2593189368 creator A5051971809 @default.
- W2593189368 creator A5072523196 @default.
- W2593189368 creator A5083420244 @default.
- W2593189368 date "2017-03-10" @default.
- W2593189368 modified "2023-10-17" @default.
- W2593189368 title "Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues" @default.
- W2593189368 cites W149540474 @default.
- W2593189368 cites W1516595608 @default.
- W2593189368 cites W1517501567 @default.
- W2593189368 cites W1544936755 @default.
- W2593189368 cites W1558627736 @default.
- W2593189368 cites W1664827751 @default.
- W2593189368 cites W1744019794 @default.
- W2593189368 cites W1791768301 @default.
- W2593189368 cites W1853590226 @default.
- W2593189368 cites W1975475795 @default.
- W2593189368 cites W1982200045 @default.
- W2593189368 cites W1985524929 @default.
- W2593189368 cites W1994279261 @default.
- W2593189368 cites W2002515133 @default.
- W2593189368 cites W2011629831 @default.
- W2593189368 cites W2012275753 @default.
- W2593189368 cites W2014449340 @default.
- W2593189368 cites W2015211029 @default.
- W2593189368 cites W2025019998 @default.
- W2593189368 cites W2030594652 @default.
- W2593189368 cites W2032371793 @default.
- W2593189368 cites W2032616796 @default.
- W2593189368 cites W2041387847 @default.
- W2593189368 cites W2052192720 @default.
- W2593189368 cites W2062235055 @default.
- W2593189368 cites W2064464428 @default.
- W2593189368 cites W2067878901 @default.
- W2593189368 cites W2072424205 @default.
- W2593189368 cites W2076532644 @default.
- W2593189368 cites W2081427336 @default.
- W2593189368 cites W2082140981 @default.
- W2593189368 cites W2082378946 @default.
- W2593189368 cites W2083260383 @default.
- W2593189368 cites W2085233986 @default.
- W2593189368 cites W2091767454 @default.
- W2593189368 cites W2096029533 @default.
- W2593189368 cites W2096898060 @default.
- W2593189368 cites W2103300312 @default.
- W2593189368 cites W2103634091 @default.
- W2593189368 cites W2104951906 @default.
- W2593189368 cites W2107053575 @default.
- W2593189368 cites W2107664383 @default.
- W2593189368 cites W2107681553 @default.
- W2593189368 cites W2117808177 @default.
- W2593189368 cites W2120331067 @default.
- W2593189368 cites W2126278579 @default.
- W2593189368 cites W2127886758 @default.
- W2593189368 cites W2128493116 @default.
- W2593189368 cites W2129638932 @default.
- W2593189368 cites W2131260695 @default.
- W2593189368 cites W2140336796 @default.
- W2593189368 cites W2145731544 @default.
- W2593189368 cites W2148245417 @default.
- W2593189368 cites W2152762406 @default.
- W2593189368 cites W2157374802 @default.
- W2593189368 cites W2157453847 @default.
- W2593189368 cites W2160648230 @default.
- W2593189368 cites W2160710430 @default.
- W2593189368 cites W2169614830 @default.
- W2593189368 cites W2170200613 @default.
- W2593189368 cites W2170274776 @default.
- W2593189368 cites W2231950910 @default.
- W2593189368 cites W2273747257 @default.
- W2593189368 cites W50972988 @default.
- W2593189368 cites W59368680 @default.
- W2593189368 cites W64930248 @default.
- W2593189368 doi "https://doi.org/10.1371/journal.pone.0173747" @default.
- W2593189368 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5345883" @default.
- W2593189368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28282455" @default.
- W2593189368 hasPublicationYear "2017" @default.
- W2593189368 type Work @default.
- W2593189368 sameAs 2593189368 @default.
- W2593189368 citedByCount "5" @default.
- W2593189368 countsByYear W25931893682017 @default.
- W2593189368 countsByYear W25931893682018 @default.
- W2593189368 countsByYear W25931893682020 @default.
- W2593189368 crossrefType "journal-article" @default.
- W2593189368 hasAuthorship W2593189368A5024015661 @default.
- W2593189368 hasAuthorship W2593189368A5026348777 @default.
- W2593189368 hasAuthorship W2593189368A5049359415 @default.
- W2593189368 hasAuthorship W2593189368A5049540949 @default.
- W2593189368 hasAuthorship W2593189368A5051971809 @default.
- W2593189368 hasAuthorship W2593189368A5072523196 @default.
- W2593189368 hasAuthorship W2593189368A5083420244 @default.
- W2593189368 hasBestOaLocation W25931893681 @default.
- W2593189368 hasConcept C116569031 @default.