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• W2593228124 endingPage "1655" @default.
• W2593228124 startingPage "1645" @default.
• W2593228124 abstract "Royal jelly (RJ) triggers the development of female honeybee larvae into queens. This effect has been attributed to the presence of major royal jelly protein 1 (MRJP1) in RJ. MRJP1 isolated from royal jelly is tightly associated with apisimin, a 54-residue α-helical peptide that promotes the noncovalent assembly of MRJP1 into multimers. No high-resolution structural data are available for these complexes, and their binding stoichiometry remains uncertain. We examined MRJP1/apisimin using a range of biophysical techniques. We also investigated the behavior of deglycosylated samples, as well as samples with reduced apisimin content. Our mass spectrometry (MS) data demonstrate that the native complexes predominantly exist in a (MRJP14 apisimin4) stoichiometry. Hydrogen/deuterium exchange MS reveals that MRJP1 within these complexes is extensively disordered in the range of residues 20-265. Marginally stable secondary structure (likely antiparallel β-sheet) exists around residues 266-432. These weakly structured regions interchange with conformers that are extensively unfolded, giving rise to bimodal (EX1) isotope distributions. We propose that the native complexes have a dimer of dimers quaternary structure in which MRJP1 chains are bridged by apisimin. Specifically, our data suggest that apisimin acts as a linker that forms hydrophobic contacts involving the MRJP1 segment 316VLFFGLV322. Deglycosylation produces large soluble aggregates, highlighting the role of glycans as aggregation inhibitors. Samples with reduced apisimin content form dimeric complexes with a (MRJP12 apisimin1) stoichiometry. The information uncovered in this work will help pave the way toward a better understanding of the unique physiological role played by MRJP1 during queen differentiation." @default.
• W2593228124 created "2017-03-16" @default.
• W2593228124 creator A5010513390 @default.
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• W2593228124 creator A5067672382 @default.
• W2593228124 creator A5076257201 @default.
• W2593228124 date "2017-03-07" @default.
• W2593228124 modified "2023-10-15" @default.
• W2593228124 title "Characterizing the Structure and Oligomerization of Major Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and Complementary Biophysical Tools" @default.
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• W2593228124 doi "https://doi.org/10.1021/acs.biochem.7b00020" @default.
• W2593228124 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5450925" @default.
• W2593228124 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28252287" @default.
• W2593228124 hasPublicationYear "2017" @default.
• W2593228124 type Work @default.

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