FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2593398483> ?p ?o ?g. }

• W2593398483 abstract "ABSTRACT To our knowledge, fecal microbiota collection methods have not been evaluated in low- and middle-income countries. Therefore, we evaluated five different fecal sample collection methods for technical reproducibility, stability, and accuracy within the Health Effects of Arsenic Longitudinal Study (HEALS) in Bangladesh. Fifty participants from the HEALS provided fecal samples in the clinic which were aliquoted into no solution, 95% ethanol, RNAlater, postdevelopment fecal occult blood test (FOBT) cards, and fecal immunochemical test (FIT) tubes. Half of the aliquots were frozen immediately at −80°C (day 0) and the remaining samples were left at ambient temperature for 96 h and then frozen (day 4). Intraclass correlation coefficients (ICC) were calculated for the relative abundances of the top three phyla, for two alpha diversity measures, and for four beta diversity measures. The duplicate samples had relatively high ICCs for technical reproducibility at day 0 and day 4 (range, 0.79 to 0.99). The FOBT card and samples preserved in RNAlater and 95% ethanol had the highest ICCs for stability over 4 days. The FIT tube had lower stability measures overall. In comparison to the “gold standard” method using immediately frozen fecal samples with no solution, the ICCs for many of the microbial metrics were low, but the rank order appeared to be preserved as seen by the Spearman correlation. The FOBT cards, 95% ethanol, and RNAlater were effective fecal preservatives. These fecal collection methods are optimal for future cohort studies, particularly in low- and middle-income countries. IMPORTANCE The collection of fecal samples in prospective cohort studies is essential to provide the opportunity to study the effect of the human microbiota on numerous health conditions. However, these collection methods have not been adequately tested in low- and middle-income countries. We present estimates of technical reproducibility, stability at ambient temperature for 4 days, and accuracy comparing a “gold standard” for fecal samples in no solution, 95% ethanol, RNAlater, postdevelopment fecal occult blood test cards, and fecal immunochemical test tubes in a study conducted in Bangladesh. Fecal occult blood test cards and fecal samples stored in 95% ethanol or RNAlater adequately preserve fecal samples in this setting. Therefore, new studies in low- and middle-income countries should include collection of fecal samples using fecal occult blood test cards, 95% ethanol, or RNAlater for prospective cohort studies." @default.
• W2593398483 created "2017-03-16" @default.
• W2593398483 creator A5003374278 @default.
• W2593398483 creator A5012823292 @default.
• W2593398483 creator A5032467174 @default.
• W2593398483 creator A5033881627 @default.
• W2593398483 creator A5038877903 @default.
• W2593398483 creator A5048679350 @default.
• W2593398483 creator A5049253513 @default.
• W2593398483 creator A5049866982 @default.
• W2593398483 creator A5052676364 @default.
• W2593398483 creator A5054369353 @default.
• W2593398483 creator A5061567478 @default.
• W2593398483 creator A5075350184 @default.
• W2593398483 creator A5077947383 @default.
• W2593398483 creator A5080956415 @default.
• W2593398483 creator A5081425275 @default.
• W2593398483 creator A5084125220 @default.
• W2593398483 creator A5089088705 @default.
• W2593398483 date "2017-05-15" @default.
• W2593398483 modified "2023-10-16" @default.
• W2593398483 title "Comparison of Fecal Collection Methods for Microbiota Studies in Bangladesh" @default.
• W2593398483 cites W1563930908 @default.
• W2593398483 cites W1682244492 @default.
• W2593398483 cites W1891948122 @default.
• W2593398483 cites W1988925586 @default.
• W2593398483 cites W2006069224 @default.
• W2593398483 cites W2037632565 @default.
• W2593398483 cites W2047304262 @default.
• W2593398483 cites W2049933107 @default.
• W2593398483 cites W2056279562 @default.
• W2593398483 cites W2063158722 @default.
• W2593398483 cites W2072970694 @default.
• W2593398483 cites W2088445262 @default.
• W2593398483 cites W2105937617 @default.
• W2593398483 cites W2119761326 @default.
• W2593398483 cites W2127013413 @default.
• W2593398483 cites W2135623916 @default.
• W2593398483 cites W2135987905 @default.
• W2593398483 cites W2149243422 @default.
• W2593398483 cites W2152751713 @default.
• W2593398483 cites W2155066423 @default.
• W2593398483 cites W2162088497 @default.
• W2593398483 cites W2165273135 @default.
• W2593398483 cites W2188930066 @default.
• W2593398483 cites W2193096068 @default.
• W2593398483 cites W2228700263 @default.
• W2593398483 cites W2252346213 @default.
• W2593398483 cites W2347104163 @default.
• W2593398483 cites W2434466422 @default.
• W2593398483 cites W2553399478 @default.
• W2593398483 cites W2564724704 @default.
• W2593398483 doi "https://doi.org/10.1128/aem.00361-17" @default.
• W2593398483 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5411505" @default.
• W2593398483 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28258145" @default.
• W2593398483 hasPublicationYear "2017" @default.
• W2593398483 type Work @default.
• W2593398483 sameAs 2593398483 @default.
• W2593398483 citedByCount "40" @default.
• W2593398483 countsByYear W25933984832017 @default.
• W2593398483 countsByYear W25933984832018 @default.
• W2593398483 countsByYear W25933984832019 @default.
• W2593398483 countsByYear W25933984832020 @default.
• W2593398483 countsByYear W25933984832021 @default.
• W2593398483 countsByYear W25933984832022 @default.
• W2593398483 countsByYear W25933984832023 @default.
• W2593398483 crossrefType "journal-article" @default.
• W2593398483 hasAuthorship W2593398483A5003374278 @default.
• W2593398483 hasAuthorship W2593398483A5012823292 @default.
• W2593398483 hasAuthorship W2593398483A5032467174 @default.
• W2593398483 hasAuthorship W2593398483A5033881627 @default.
• W2593398483 hasAuthorship W2593398483A5038877903 @default.
• W2593398483 hasAuthorship W2593398483A5048679350 @default.
• W2593398483 hasAuthorship W2593398483A5049253513 @default.
• W2593398483 hasAuthorship W2593398483A5049866982 @default.
• W2593398483 hasAuthorship W2593398483A5052676364 @default.
• W2593398483 hasAuthorship W2593398483A5054369353 @default.
• W2593398483 hasAuthorship W2593398483A5061567478 @default.
• W2593398483 hasAuthorship W2593398483A5075350184 @default.
• W2593398483 hasAuthorship W2593398483A5077947383 @default.
• W2593398483 hasAuthorship W2593398483A5080956415 @default.
• W2593398483 hasAuthorship W2593398483A5081425275 @default.
• W2593398483 hasAuthorship W2593398483A5084125220 @default.
• W2593398483 hasAuthorship W2593398483A5089088705 @default.
• W2593398483 hasBestOaLocation W25933984831 @default.
• W2593398483 hasConcept C104709138 @default.
• W2593398483 hasConcept C105795698 @default.
• W2593398483 hasConcept C124956284 @default.
• W2593398483 hasConcept C140793950 @default.
• W2593398483 hasConcept C18903297 @default.
• W2593398483 hasConcept C2780797713 @default.
• W2593398483 hasConcept C33923547 @default.
• W2593398483 hasConcept C61716771 @default.
• W2593398483 hasConcept C71924100 @default.
• W2593398483 hasConcept C86803240 @default.
• W2593398483 hasConcept C89423630 @default.
• W2593398483 hasConcept C9893847 @default.
• W2593398483 hasConceptScore W2593398483C104709138 @default.
• W2593398483 hasConceptScore W2593398483C105795698 @default.
• W2593398483 hasConceptScore W2593398483C124956284 @default.

• next