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- W2593582015 abstract "The aim of this contribution was focused on the isolation ofPCR-ready DNA from Trichophyton lyophilised cells (spores) andfrom the small volumes of Staphylococcus aureus phage lysates.Extraction of PCR-ready DNA from microscopic fungi is crucialin dermatophytes due to the presence of fungal polysaccharidesand the chitinous cell wall. PCR-ready DNA isolated from crudecell lysates was repurified by magnetic carriers containingcarboxyl groups on the surface. The adsorbed DNA was eluted byTE buffer. The DNA in the eluate was used as a matrix in PCRamplification. ITS4 and ITS5 primers were used for theamplification of Trichophyton DNA. The PCR-ready DNA can beisolated from lyophilisates without cultivation of cells(spores) and the influence of PCR inhibitors which are presentin lyophilisation media was eliminated. The high-titre (109particles per ml) and low-titre (103 particles per ml) phagelysates of Staphylococcus aureus bacteriophages f77 and f53were used for sample preparation and DNA isolation. The phagelysates were treated by proteinase K. The DNA extracted usingmagnetic microspheres was RNA-free and multiplex PCR was usedfor phage DNA identification." @default.
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- W2593582015 date "2008-01-01" @default.
- W2593582015 modified "2023-09-27" @default.
- W2593582015 title "Isolation of PCR-ready DNA from lyophilisates of Trichophytonfungi and from phages of Staphylococcus aureus using magneticmicrospheres" @default.
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