FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2593672643> ?p ?o ?g. }

• W2593672643 endingPage "E525" @default.
• W2593672643 startingPage "E524" @default.
• W2593672643 abstract "Tumor treating fields (TTFields) are an established anti-neoplastic treatment modality in patients with Glioblastoma Multiforme brain tumors. TTFields are delivered via noninvasive application of low-intensity (1-3 V/cm), intermediate-frequency (100-300 kHz), and alternating electric fields to the region of the tumor. The combination of TTFields with chemotherapeutic agents has resulted in enhanced anti-neoplastic efficacy across a variety of tumor cell lines, as well as enhanced treatment efficacy in malignant tumors in vivo. To date, no study has examined the outcomes of combining TTFields exposure with radiation therapy (RT) and thus the therapeutic potential of such combination remains unknown. To investigate whether TTFields can improve RT treatment outcomes, we studied glioma cells treated with RT in combination with TTFields. To evaluate the efficacy of combined treatment with TTFields and RT, the surviving fractions of U-118 MG and U-87 MG glioma cells were determined. Cells were treated for 72h with TTFields (1.75 V/cm RMS, 200 kHz) applied immediately, 1, 4 or 24 h after irradiation (IR) in 4Gy or in different doses. Surviving fractions were calculated. For assessment of DNA double strand breaks, cells were stained with anti-γH2AX antibody or anti-Rad51 antibody. Immunoblot analysis was used for the assessment of activation pattern of phospho-ATM (pS1981) and phospho-DNA-PK (pS2056). Functionality of non-homologous end-joining (NHEJ) and micro-homologous DNA damage repair was assayed using pGL2-Luc vector linearized with either HindIII or EcoRI. Luciferase activity was evaluated using the dual luciferase reporter assay system. The radiomimetic agent bleomycin was used in combination with TTFields to specifically assess the effect of TTFields on double strand breaks (DSB). Alkaline comet assay was used to measure DNA lesions in cells treated with TTFields following exposure to bleomycin or RT. RT treatment efficacy was significantly enhanced when combined with TTFields therapy. The combination therapy was found to be most effective when TTFields were applied immediately after exposure to RT. On the molecular level, our results demonstrate aberrant DNA damage repair and extended retention of γH2AX and Rad51 nuclear foci in cells 24 hours after exposure to both modalities. NHEJ and microhomologous DNA damage repair were not significantly affected by TTFields. Our results suggest that in addition to exerting an anti-mitotic effect, TTFields radiosensitize glioma cells by specifically reducing IR induced DNA damage repair by homologous recombination, providing a strong rationale to investigate the use of TTFields in combination with RT in the clinical setting." @default.
• W2593672643 created "2017-03-16" @default.
• W2593672643 creator A5005523374 @default.
• W2593672643 creator A5007967617 @default.
• W2593672643 creator A5017815724 @default.
• W2593672643 creator A5021566903 @default.
• W2593672643 creator A5021783438 @default.
• W2593672643 creator A5042212699 @default.
• W2593672643 creator A5056907794 @default.
• W2593672643 creator A5057970471 @default.
• W2593672643 creator A5083247843 @default.
• W2593672643 creator A5087293190 @default.
• W2593672643 creator A5089132013 @default.
• W2593672643 date "2015-11-01" @default.
• W2593672643 modified "2023-10-17" @default.
• W2593672643 title "Tumor Treating Fields (TTFields) Sensitize Glioma Tumor Cells to Radiation Therapy by Delaying DNA Damage Repair Through Homologous Recombination" @default.
• W2593672643 doi "https://doi.org/10.1016/j.ijrobp.2015.07.1888" @default.
• W2593672643 hasPublicationYear "2015" @default.
• W2593672643 type Work @default.
• W2593672643 sameAs 2593672643 @default.
• W2593672643 citedByCount "6" @default.
• W2593672643 countsByYear W25936726432017 @default.
• W2593672643 countsByYear W25936726432018 @default.
• W2593672643 countsByYear W25936726432020 @default.
• W2593672643 countsByYear W25936726432021 @default.
• W2593672643 crossrefType "journal-article" @default.
• W2593672643 hasAuthorship W2593672643A5005523374 @default.
• W2593672643 hasAuthorship W2593672643A5007967617 @default.
• W2593672643 hasAuthorship W2593672643A5017815724 @default.
• W2593672643 hasAuthorship W2593672643A5021566903 @default.
• W2593672643 hasAuthorship W2593672643A5021783438 @default.
• W2593672643 hasAuthorship W2593672643A5042212699 @default.
• W2593672643 hasAuthorship W2593672643A5056907794 @default.
• W2593672643 hasAuthorship W2593672643A5057970471 @default.
• W2593672643 hasAuthorship W2593672643A5083247843 @default.
• W2593672643 hasAuthorship W2593672643A5087293190 @default.
• W2593672643 hasAuthorship W2593672643A5089132013 @default.
• W2593672643 hasBestOaLocation W25936726431 @default.
• W2593672643 hasConcept C102744134 @default.
• W2593672643 hasConcept C126322002 @default.
• W2593672643 hasConcept C150903083 @default.
• W2593672643 hasConcept C153911025 @default.
• W2593672643 hasConcept C207001950 @default.
• W2593672643 hasConcept C2778227246 @default.
• W2593672643 hasConcept C2779012473 @default.
• W2593672643 hasConcept C502942594 @default.
• W2593672643 hasConcept C509974204 @default.
• W2593672643 hasConcept C54355233 @default.
• W2593672643 hasConcept C552990157 @default.
• W2593672643 hasConcept C71924100 @default.
• W2593672643 hasConcept C86803240 @default.
• W2593672643 hasConceptScore W2593672643C102744134 @default.
• W2593672643 hasConceptScore W2593672643C126322002 @default.
• W2593672643 hasConceptScore W2593672643C150903083 @default.
• W2593672643 hasConceptScore W2593672643C153911025 @default.
• W2593672643 hasConceptScore W2593672643C207001950 @default.
• W2593672643 hasConceptScore W2593672643C2778227246 @default.
• W2593672643 hasConceptScore W2593672643C2779012473 @default.
• W2593672643 hasConceptScore W2593672643C502942594 @default.
• W2593672643 hasConceptScore W2593672643C509974204 @default.
• W2593672643 hasConceptScore W2593672643C54355233 @default.
• W2593672643 hasConceptScore W2593672643C552990157 @default.
• W2593672643 hasConceptScore W2593672643C71924100 @default.
• W2593672643 hasConceptScore W2593672643C86803240 @default.
• W2593672643 hasIssue "3" @default.
• W2593672643 hasLocation W25936726431 @default.
• W2593672643 hasOpenAccess W2593672643 @default.
• W2593672643 hasPrimaryLocation W25936726431 @default.
• W2593672643 hasRelatedWork W1961425141 @default.
• W2593672643 hasRelatedWork W1981244332 @default.
• W2593672643 hasRelatedWork W2001696529 @default.
• W2593672643 hasRelatedWork W2022421686 @default.
• W2593672643 hasRelatedWork W2035688196 @default.
• W2593672643 hasRelatedWork W2055546213 @default.
• W2593672643 hasRelatedWork W2076225736 @default.
• W2593672643 hasRelatedWork W2083843455 @default.
• W2593672643 hasRelatedWork W2085975659 @default.
• W2593672643 hasRelatedWork W2326915229 @default.
• W2593672643 hasVolume "93" @default.
• W2593672643 isParatext "false" @default.
• W2593672643 isRetracted "false" @default.
• W2593672643 magId "2593672643" @default.
• W2593672643 workType "article" @default.