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• W2593726210 abstract "Cultured luteal cells are profound stimulators of T lymphocyte (T cell) proliferation, and the gamma-delta subset of T cells is the most responsive to stimulation by luteal cells. It has been shown that multiple signals are required for gamma-delta T cell activation but the factors have not been well defined, and gamma-delta T cells are not thought to be MHC restricted. Luteal cells induce proliferation of gamma-delta T cells in vitro by direct contact. It was of interest to determine if products of the luteal cells are involved in the paracrine regulation of gamma-delta T cell activation. The first objective of this study was to determine if luteal cell secretions could stimulate proliferation of T cells. Corpora lutea were collected from cyclic cattle on day 10 of the estrous cycle, dissociated and placed in culture for 48 hours. T lymphocytes were isolated from the blood of the same cows immunomagnetically and were exposed to luteal conditioned medium. All experiments were repeated 3 times. P<0.05 was used to define differences. Proliferation of gamma-delta T cells was increased significantly by luteal conditioned medium (60%) when compared to T cells cultured in fresh medium (15%). The second objective was to determine which soluble factor(s) secreted from luteal cells was able to induce the response of gamma-delta T cells. The luteal conditioned media were subjected to high temperature, digested by trypsin immobilized on magnetic beads, and concentrated on centrifugal filters with different molecular weight cut-offs (3, 10, 30, 50 and 100 kDa). The profile of secreted proteins in conditioned medium was then analyzed by SDS-PAGE and mass spectrometry analysis. These data show the following: (1) Exposure of conditioned medium to 95oC for 30 min partly inactivated proteins and decreased gamma-delta T cell proliferation to 35%. The gamma-delta T cell response to media heated with other temperatures (65oC and 45oC) was unchanged. (2) The soluble factor(s) in medium did not stimulate T cell proliferation (10%) after 2 hours of mag-trypsin digestion. (3) The retained fractions with molecules above a specified molecular weight, collected after concentration, showed that the major factor(s) that induced gamma-delta T cell proliferation had a relative molecular mass of approximately 100kDa or above. It can be concluded from this study that the factor(s) secreted by luteal cells that stimulates T cell proliferation is heat labile, trypsin-sensitive and a large molecular weight. It is concluded that luteal cells secrete a large protein that acts in a paracrine manner to activate T cells. This project was supported by National Research Initiative Competitive Grant no. 2008-35203-04617 from the USDA National Institute of Food and Agriculture. (poster)" @default.
• W2593726210 created "2017-03-16" @default.
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• W2593726210 date "2011-07-01" @default.
• W2593726210 modified "2023-10-18" @default.
• W2593726210 title "Luteal Cells Induce Gamma Delta T Cell Activation Through Secretory Factors." @default.
• W2593726210 doi "https://doi.org/10.1093/biolreprod/85.s1.222" @default.
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