Matches in SemOpenAlex for { <https://semopenalex.org/work/W2593754901> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2593754901 abstract "The HCN ion channel subunit gene family encodes hyperpolarization-activated cation channels that are permeable to Na + and K + . There are four members of this channel family, three of which, HCN1, HCN2, and HCN4, are expressed in the heart. Current evidence suggests that the HCN ion channel subunit family is the molecular correlate of the alpha subunit of the cardiac pacemaker current if. Our previous work has shown that HCN4 is the dominant isoform expressed in the rabbit sinoatrial (SA) node and that changes in tyrosine phos- phorylation, either by kinase inhibition or growth factor activation, lead to changes in rabbit SA node if conduc- tance with no change in voltage dependence. In the present study we investigate the actions of genistein, a tyrosine kinase inhibitor, on heterologously expressed HCN currents in Xenopus oocytes. Genistein had no effect on HCN1-induced currents, but reduced whole-cell currents induced by HCN2 or HCN4 and slowed activation kinetics at voltages near the midpoint of activation. In the case of HCN2 there was also a negative shift in the voltage dependence of activation that accompanies the current reduction. We have shown previously that HCN2 is the dominant isoform expressed in rat ventricular myocytes. The above results predict that genistein should reduce if in the rat ventricle and cause a negative shift of voltage dependence and kinetics of activation. We tested this hypothesis by studying the effects of genistein on isolated rat ventricular myocytes. Genistein significantly reduced if current density (pA/pF) (control: 12.2±1.8; genistein: 3.5 ±0.5; washout: 7.7±0.8; n=10), and caused a negative shift of the midpoint of activation by 14 mV (�133±1 mV for genistein and �119±1 mV for washout, n=7) with no change in slope factor. Our results thus suggest that if in the heart and if-like currents in other tissues can be regulated differentially by tyrosine phosphorylation based on isoform expression patterns." @default.
- W2593754901 created "2017-03-16" @default.
- W2593754901 creator A5001441830 @default.
- W2593754901 creator A5021250050 @default.
- W2593754901 creator A5039715577 @default.
- W2593754901 date "2004-01-01" @default.
- W2593754901 modified "2023-10-18" @default.
- W2593754901 title "CELL AND MOLECULAR PHYSIOLOGY" @default.
- W2593754901 cites W1893444436 @default.
- W2593754901 cites W1974273055 @default.
- W2593754901 cites W1988080361 @default.
- W2593754901 cites W2002632985 @default.
- W2593754901 cites W2009818140 @default.
- W2593754901 cites W2010048605 @default.
- W2593754901 cites W2010288352 @default.
- W2593754901 cites W2010973571 @default.
- W2593754901 cites W2038163496 @default.
- W2593754901 cites W2076043284 @default.
- W2593754901 cites W2084471888 @default.
- W2593754901 cites W2091122073 @default.
- W2593754901 cites W2094531569 @default.
- W2593754901 cites W2102650234 @default.
- W2593754901 cites W2105326956 @default.
- W2593754901 cites W2110233921 @default.
- W2593754901 cites W2157578689 @default.
- W2593754901 hasPublicationYear "2004" @default.
- W2593754901 type Work @default.
- W2593754901 sameAs 2593754901 @default.
- W2593754901 citedByCount "0" @default.
- W2593754901 crossrefType "journal-article" @default.
- W2593754901 hasAuthorship W2593754901A5001441830 @default.
- W2593754901 hasAuthorship W2593754901A5021250050 @default.
- W2593754901 hasAuthorship W2593754901A5039715577 @default.
- W2593754901 hasConcept C104292427 @default.
- W2593754901 hasConcept C104317684 @default.
- W2593754901 hasConcept C12554922 @default.
- W2593754901 hasConcept C126322002 @default.
- W2593754901 hasConcept C134018914 @default.
- W2593754901 hasConcept C170493617 @default.
- W2593754901 hasConcept C177832589 @default.
- W2593754901 hasConcept C185592680 @default.
- W2593754901 hasConcept C207200792 @default.
- W2593754901 hasConcept C2778298627 @default.
- W2593754901 hasConcept C2779535977 @default.
- W2593754901 hasConcept C4141045 @default.
- W2593754901 hasConcept C50254741 @default.
- W2593754901 hasConcept C53345823 @default.
- W2593754901 hasConcept C55493867 @default.
- W2593754901 hasConcept C71924100 @default.
- W2593754901 hasConcept C86803240 @default.
- W2593754901 hasConceptScore W2593754901C104292427 @default.
- W2593754901 hasConceptScore W2593754901C104317684 @default.
- W2593754901 hasConceptScore W2593754901C12554922 @default.
- W2593754901 hasConceptScore W2593754901C126322002 @default.
- W2593754901 hasConceptScore W2593754901C134018914 @default.
- W2593754901 hasConceptScore W2593754901C170493617 @default.
- W2593754901 hasConceptScore W2593754901C177832589 @default.
- W2593754901 hasConceptScore W2593754901C185592680 @default.
- W2593754901 hasConceptScore W2593754901C207200792 @default.
- W2593754901 hasConceptScore W2593754901C2778298627 @default.
- W2593754901 hasConceptScore W2593754901C2779535977 @default.
- W2593754901 hasConceptScore W2593754901C4141045 @default.
- W2593754901 hasConceptScore W2593754901C50254741 @default.
- W2593754901 hasConceptScore W2593754901C53345823 @default.
- W2593754901 hasConceptScore W2593754901C55493867 @default.
- W2593754901 hasConceptScore W2593754901C71924100 @default.
- W2593754901 hasConceptScore W2593754901C86803240 @default.
- W2593754901 hasLocation W25937549011 @default.
- W2593754901 hasOpenAccess W2593754901 @default.
- W2593754901 hasPrimaryLocation W25937549011 @default.
- W2593754901 hasRelatedWork W1608854978 @default.
- W2593754901 hasRelatedWork W1989268481 @default.
- W2593754901 hasRelatedWork W2005257843 @default.
- W2593754901 hasRelatedWork W2011532113 @default.
- W2593754901 hasRelatedWork W2016582329 @default.
- W2593754901 hasRelatedWork W2018947384 @default.
- W2593754901 hasRelatedWork W2040987653 @default.
- W2593754901 hasRelatedWork W2048725277 @default.
- W2593754901 hasRelatedWork W2070242177 @default.
- W2593754901 hasRelatedWork W2089973072 @default.
- W2593754901 hasRelatedWork W2098819415 @default.
- W2593754901 hasRelatedWork W2111251260 @default.
- W2593754901 hasRelatedWork W2111306466 @default.
- W2593754901 hasRelatedWork W2156152070 @default.
- W2593754901 hasRelatedWork W2295087188 @default.
- W2593754901 hasRelatedWork W2403483497 @default.
- W2593754901 hasRelatedWork W2423815963 @default.
- W2593754901 hasRelatedWork W2891319725 @default.
- W2593754901 hasRelatedWork W3005636757 @default.
- W2593754901 hasRelatedWork W3144484953 @default.
- W2593754901 isParatext "false" @default.
- W2593754901 isRetracted "false" @default.
- W2593754901 magId "2593754901" @default.
- W2593754901 workType "article" @default.