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- W2593782848 abstract "The use of the nuclear DNA reassociation technique has led taxonomists to consider Saccharomyces uvarum a synonym of S. bayanus. The latter, however, is not a species but a hybrid harbouring S. eubayanus (Seu) and S. uvarum (Su) subgenomes with a minor DNA contribution from S. cerevisiae (Sc). To recognize genetically pure lines of S. uvarum and putative interspecies hybrids among so-called S. bayanus strains present in public culture collections, we propose the use of four markers that were defined from the S. bayanus CBS 380T composite genome, namely SeuNTS2 (rDNA), ScMAL31, MTY1 and SuMEL1. Saccharomyces carlsbergensis CBS 1513 was found to be similar to S. bayanus except that it carries the SeuMEL1 allele. Different marker combinations revealed that among 33 strains examined only a few were similar to CBS 380T, but many pure S. uvarum lines and putative Su/Seu-related hybrids occurred. Our results demonstrated that these hybrids were erroneously considered authentic S. bayanus and therefore the varietal state ‘Saccharomyces bayanus var. uvarum comb. nov. Naumov’ is not valid. Our markers constitute a tool to get insights into the genomic makeup of Saccharomyces interspecies hybrids. We also make a proposal to name those hybrids that may also be applicable to other fungal hybrids." @default.
- W2593782848 created "2017-03-16" @default.
- W2593782848 creator A5032472915 @default.
- W2593782848 creator A5036101186 @default.
- W2593782848 date "2017-03-01" @default.
- W2593782848 modified "2023-10-17" @default.
- W2593782848 title "Characterization of Saccharomyces uvarum (Beijerinck, 1898) and related hybrids: assessment of molecular markers that predict the parent and hybrid genomes and a proposal to name yeast hybrids" @default.
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- W2593782848 doi "https://doi.org/10.1093/femsyr/fox014" @default.
- W2593782848 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28334169" @default.
- W2593782848 hasPublicationYear "2017" @default.
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