FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2594090180> ?p ?o ?g. }

• W2594090180 endingPage "e0005433" @default.
• W2594090180 startingPage "e0005433" @default.
• W2594090180 abstract "The performance of current serologic tests for diagnosing chronic Chagas disease (CD) is highly variable. The search for new diagnostic markers has been a constant challenge for improving accuracy and reducing the number of inconclusive results.Here, four chimeric proteins (IBMP-8.1 to -8.4) comprising immunodominant regions of different Trypanosoma cruzi antigens were tested by enzyme-linked immunosorbent assay. The proteins were used to detect specific anti-T. cruzi antibodies in the sera of 857 chagasic and 689 non-chagasic individuals to evaluate their accuracy for chronic CD diagnosis. The antigens were recombinantly expressed in Escherichia coli and purified by chromatographic methods. The sensitivity and specificity values ranged from 94.3% to 99.3% and 99.4% to 100%, respectively. The diagnostic odds ratio (DOR) values were 6,462 for IBMP-8.1, 3,807 for IBMP-8.2, 32,095 for IBMP-8.3, and 283,714 for IBMP-8.4. These chimeric antigens presented DORs that were higher than the commercial test Pathozyme Chagas. The antigens IBMP-8.3 and -8.4 also showed DORs higher than the Gold ELISA Chagas test. Mixtures with equimolar concentrations were tested in order to improve the diagnosis accuracy of negative samples with high signal and positive samples with low signal. However, no gain in accuracy was observed relative to the individual antigens. A total of 1,079 additional sera were used to test cross-reactivity to unrelated diseases. The cross-reactivity rates ranged from 0.37% to 0.74% even for Leishmania spp., a pathogen showing relatively high genome sequence identity to T. cruzi. Imprecision analyses showed that IBMP chimeras are very stable and the results are highly reproducible.Our findings indicate that the IBMP-8.4 antigen can be safely used in serological tests for T. cruzi screening in blood banks and for chronic CD laboratory diagnosis." @default.
• W2594090180 created "2017-03-16" @default.
• W2594090180 creator A5006091022 @default.
• W2594090180 creator A5007076202 @default.
• W2594090180 creator A5008758672 @default.
• W2594090180 creator A5015354831 @default.
• W2594090180 creator A5042733686 @default.
• W2594090180 creator A5074172780 @default.
• W2594090180 creator A5085569238 @default.
• W2594090180 creator A5088289702 @default.
• W2594090180 date "2017-03-08" @default.
• W2594090180 modified "2023-10-13" @default.
• W2594090180 title "Accuracy of chimeric proteins in the serological diagnosis of chronic chagas disease – a Phase II study" @default.
• W2594090180 cites W1754090969 @default.
• W2594090180 cites W1934811695 @default.
• W2594090180 cites W1967906311 @default.
• W2594090180 cites W1970205620 @default.
• W2594090180 cites W1972043575 @default.
• W2594090180 cites W1976609481 @default.
• W2594090180 cites W1977946740 @default.
• W2594090180 cites W1978426257 @default.
• W2594090180 cites W1979387782 @default.
• W2594090180 cites W1979442081 @default.
• W2594090180 cites W1984734982 @default.
• W2594090180 cites W1989793699 @default.
• W2594090180 cites W2015647554 @default.
• W2594090180 cites W2019634118 @default.
• W2594090180 cites W2030666209 @default.
• W2594090180 cites W2038985997 @default.
• W2594090180 cites W2046460184 @default.
• W2594090180 cites W2061246349 @default.
• W2594090180 cites W2063524276 @default.
• W2594090180 cites W2065002789 @default.
• W2594090180 cites W2069057074 @default.
• W2594090180 cites W2085095908 @default.
• W2594090180 cites W2087734610 @default.
• W2594090180 cites W2092712542 @default.
• W2594090180 cites W2092926517 @default.
• W2594090180 cites W2098082628 @default.
• W2594090180 cites W2105827340 @default.
• W2594090180 cites W2106485985 @default.
• W2594090180 cites W2126602963 @default.
• W2594090180 cites W2132390016 @default.
• W2594090180 cites W2136019705 @default.
• W2594090180 cites W2138286403 @default.
• W2594090180 cites W2145163132 @default.
• W2594090180 cites W2149258299 @default.
• W2594090180 cites W2155883358 @default.
• W2594090180 cites W2164777277 @default.
• W2594090180 cites W2164874656 @default.
• W2594090180 cites W2172105162 @default.
• W2594090180 cites W2222811189 @default.
• W2594090180 cites W2223995057 @default.
• W2594090180 cites W2265581766 @default.
• W2594090180 cites W2287494264 @default.
• W2594090180 cites W2318935314 @default.
• W2594090180 cites W2322332255 @default.
• W2594090180 cites W2406994565 @default.
• W2594090180 cites W2409283406 @default.
• W2594090180 cites W2411154732 @default.
• W2594090180 cites W2417222141 @default.
• W2594090180 cites W2508487539 @default.
• W2594090180 cites W4233511858 @default.
• W2594090180 cites W4299960106 @default.
• W2594090180 doi "https://doi.org/10.1371/journal.pntd.0005433" @default.
• W2594090180 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5358787" @default.
• W2594090180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28273127" @default.
• W2594090180 hasPublicationYear "2017" @default.
• W2594090180 type Work @default.
• W2594090180 sameAs 2594090180 @default.
• W2594090180 citedByCount "26" @default.
• W2594090180 countsByYear W25940901802017 @default.
• W2594090180 countsByYear W25940901802018 @default.
• W2594090180 countsByYear W25940901802019 @default.
• W2594090180 countsByYear W25940901802020 @default.
• W2594090180 countsByYear W25940901802021 @default.
• W2594090180 countsByYear W25940901802022 @default.
• W2594090180 countsByYear W25940901802023 @default.
• W2594090180 crossrefType "journal-article" @default.
• W2594090180 hasAuthorship W2594090180A5006091022 @default.
• W2594090180 hasAuthorship W2594090180A5007076202 @default.
• W2594090180 hasAuthorship W2594090180A5008758672 @default.
• W2594090180 hasAuthorship W2594090180A5015354831 @default.
• W2594090180 hasAuthorship W2594090180A5042733686 @default.
• W2594090180 hasAuthorship W2594090180A5074172780 @default.
• W2594090180 hasAuthorship W2594090180A5085569238 @default.
• W2594090180 hasAuthorship W2594090180A5088289702 @default.
• W2594090180 hasBestOaLocation W25940901801 @default.
• W2594090180 hasConcept C112418997 @default.
• W2594090180 hasConcept C136764020 @default.
• W2594090180 hasConcept C147483822 @default.
• W2594090180 hasConcept C159047783 @default.
• W2594090180 hasConcept C159654299 @default.
• W2594090180 hasConcept C203014093 @default.
• W2594090180 hasConcept C2777625466 @default.
• W2594090180 hasConcept C2780420688 @default.
• W2594090180 hasConcept C41008148 @default.
• W2594090180 hasConcept C45189115 @default.

• next