Matches in SemOpenAlex for { <https://semopenalex.org/work/W2594539021> ?p ?o ?g. }
- W2594539021 endingPage "e0173400" @default.
- W2594539021 startingPage "e0173400" @default.
- W2594539021 abstract "Using unique computer-assisted 3D reconstruction software, it was previously demonstrated that tumorigenic cell lines derived from breast tumors, when seeded in a 3D Matrigel model, grew as clonal aggregates which, after approximately 100 hours, underwent coalescence mediated by specialized cells, eventually forming a highly structured large spheroid. Non-tumorigenic cells did not undergo coalescence. Because histological sections of melanomas forming in patients suggest that melanoma cells migrate and coalesce to form tumors, we tested whether they also underwent coalescence in a 3D Matrigel model. Melanoma cells exiting fragments of three independent melanomas or from secondary cultures derived from them, and cells from the melanoma line HTB-66, all underwent coalescence mediated by specialized cells in the 3D model. Normal melanocytes did not. However, coalescence of melanoma cells differed from that of breast-derived tumorigenic cell lines in that they 1) coalesced immediately, 2) underwent coalescence as individual cells as well as aggregates, 3) underwent coalescence far faster and 4) ultimately formed long, flat, fenestrated aggregates that were extremely dynamic. A screen of 51 purified monoclonal antibodies (mAbs) targeting cell surface-associated molecules revealed that two mAbs, anti-beta 1 integrin/(CD29) and anti-CD44, blocked melanoma cell coalescence. They also blocked coalescence of tumorigenic cells derived from a breast tumor. These results add weight to the commonality of coalescence as a characteristic of tumorigenic cells, as well as the usefulness of the 3D Matrigel model and software for both investigating the mechanisms regulating tumorigenesis and screening for potential anti-tumorigenesis mAbs." @default.
- W2594539021 created "2017-03-16" @default.
- W2594539021 creator A5001607626 @default.
- W2594539021 creator A5010753904 @default.
- W2594539021 creator A5015130871 @default.
- W2594539021 creator A5036353840 @default.
- W2594539021 creator A5041873727 @default.
- W2594539021 creator A5046544722 @default.
- W2594539021 creator A5056940317 @default.
- W2594539021 creator A5070814505 @default.
- W2594539021 creator A5074985509 @default.
- W2594539021 creator A5079572031 @default.
- W2594539021 creator A5083829432 @default.
- W2594539021 creator A5089777560 @default.
- W2594539021 date "2017-03-06" @default.
- W2594539021 modified "2023-09-26" @default.
- W2594539021 title "Melanoma cells undergo aggressive coalescence in a 3D Matrigel model that is repressed by anti-CD44" @default.
- W2594539021 cites W1510510224 @default.
- W2594539021 cites W1545715882 @default.
- W2594539021 cites W1589415602 @default.
- W2594539021 cites W1834998257 @default.
- W2594539021 cites W1888764902 @default.
- W2594539021 cites W1949238489 @default.
- W2594539021 cites W1967120404 @default.
- W2594539021 cites W1969488570 @default.
- W2594539021 cites W1971139108 @default.
- W2594539021 cites W1974391793 @default.
- W2594539021 cites W1975472487 @default.
- W2594539021 cites W1976400563 @default.
- W2594539021 cites W1977604024 @default.
- W2594539021 cites W1980218077 @default.
- W2594539021 cites W1981180318 @default.
- W2594539021 cites W1982393145 @default.
- W2594539021 cites W1983757617 @default.
- W2594539021 cites W1984519260 @default.
- W2594539021 cites W1985585224 @default.
- W2594539021 cites W1987659275 @default.
- W2594539021 cites W1990578192 @default.
- W2594539021 cites W1991949008 @default.
- W2594539021 cites W1994990789 @default.
- W2594539021 cites W1995168697 @default.
- W2594539021 cites W1998926479 @default.
- W2594539021 cites W2002333819 @default.
- W2594539021 cites W2003436264 @default.
- W2594539021 cites W2007904495 @default.
- W2594539021 cites W2008951519 @default.
- W2594539021 cites W2013359277 @default.
- W2594539021 cites W2015937963 @default.
- W2594539021 cites W2017559015 @default.
- W2594539021 cites W2018580411 @default.
- W2594539021 cites W2021758180 @default.
- W2594539021 cites W2023186669 @default.
- W2594539021 cites W2023427658 @default.
- W2594539021 cites W2025192293 @default.
- W2594539021 cites W2025529909 @default.
- W2594539021 cites W2029256820 @default.
- W2594539021 cites W2030584536 @default.
- W2594539021 cites W2032951366 @default.
- W2594539021 cites W2034269086 @default.
- W2594539021 cites W2034617750 @default.
- W2594539021 cites W2036042116 @default.
- W2594539021 cites W2037632318 @default.
- W2594539021 cites W2040972825 @default.
- W2594539021 cites W2042187915 @default.
- W2594539021 cites W2045306157 @default.
- W2594539021 cites W2050957113 @default.
- W2594539021 cites W2051641864 @default.
- W2594539021 cites W2052593409 @default.
- W2594539021 cites W2053704624 @default.
- W2594539021 cites W2057341802 @default.
- W2594539021 cites W2058692868 @default.
- W2594539021 cites W2059469601 @default.
- W2594539021 cites W2068264488 @default.
- W2594539021 cites W2068961038 @default.
- W2594539021 cites W2074512253 @default.
- W2594539021 cites W2076648223 @default.
- W2594539021 cites W2077282333 @default.
- W2594539021 cites W2078003307 @default.
- W2594539021 cites W2078607328 @default.
- W2594539021 cites W2084169296 @default.
- W2594539021 cites W2089701444 @default.
- W2594539021 cites W2094098398 @default.
- W2594539021 cites W2094830604 @default.
- W2594539021 cites W2094865209 @default.
- W2594539021 cites W2099340085 @default.
- W2594539021 cites W2102838769 @default.
- W2594539021 cites W2104902501 @default.
- W2594539021 cites W2112327424 @default.
- W2594539021 cites W2116842737 @default.
- W2594539021 cites W2117692326 @default.
- W2594539021 cites W2118815929 @default.
- W2594539021 cites W2128161552 @default.
- W2594539021 cites W2131827318 @default.
- W2594539021 cites W2134822799 @default.
- W2594539021 cites W2139082708 @default.
- W2594539021 cites W2145371947 @default.
- W2594539021 cites W2147000598 @default.
- W2594539021 cites W2151496433 @default.