FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2594774920> ?p ?o ?g. }

• W2594774920 endingPage "147" @default.
• W2594774920 startingPage "137" @default.
• W2594774920 abstract "C3b, the central component of the alternative pathway (AP) of the complement system, coexists as a mixture of conformations in solution. These conformational changes can affect interactions with other proteins and complement regulators. Here we combine a computational model for electrostatic interactions within C3b with molecular imaging to study the conformation of C3b. The computational analysis shows that the TED domain in C3b is tethered ionically to the macroglobulin (MG) ring. Monovalent counterion concentration affects the magnitude of electrostatic forces anchoring the TED domain to the rest of the C3b molecule in a thermodynamic model. This is confirmed by observing NaCl concentration dependent conformational changes using single molecule electron microscopy (EM). We show that the displacement of the TED domain is compatible with C3b binding to Factor B (FB), suggesting that the regulation of the C3bBb convertase could be affected by conditions that promote movement in the TED domain. Our molecular model also predicts mutations that could alter the positioning of the TED domain, including the common R102G polymorphism, a risk variant for developing age-related macular degeneration. The common C3b isoform, C3bS, and the risk isoform, C3bF, show distinct energetic barriers to displacement in the TED that are related to a network of electrostatic interactions at the interface of the TED and MG-ring domains of C3b. These computational predictions agree with experimental evidence that shows differences in conformation observed in C3b isoforms purified from homozygous donors. Altogether, we reveal an ionic, reversible attachment of the TED domain to the MG ring that may influence complement regulation in some mutations and polymorphisms of C3b." @default.
• W2594774920 created "2017-03-16" @default.
• W2594774920 creator A5009690143 @default.
• W2594774920 creator A5011009080 @default.
• W2594774920 creator A5052276722 @default.
• W2594774920 creator A5053679121 @default.
• W2594774920 creator A5057157143 @default.
• W2594774920 creator A5090077495 @default.
• W2594774920 creator A5091384341 @default.
• W2594774920 date "2017-05-01" @default.
• W2594774920 modified "2023-10-12" @default.
• W2594774920 title "Ionic tethering contributes to the conformational stability and function of complement C3b" @default.
• W2594774920 cites W1031578623 @default.
• W2594774920 cites W1966887649 @default.
• W2594774920 cites W1974073556 @default.
• W2594774920 cites W1979551570 @default.
• W2594774920 cites W1980374511 @default.
• W2594774920 cites W1982407325 @default.
• W2594774920 cites W1986074549 @default.
• W2594774920 cites W1990643927 @default.
• W2594774920 cites W1992861835 @default.
• W2594774920 cites W1998016702 @default.
• W2594774920 cites W1998909987 @default.
• W2594774920 cites W1998925734 @default.
• W2594774920 cites W2011658408 @default.
• W2594774920 cites W2029582401 @default.
• W2594774920 cites W2032145111 @default.
• W2594774920 cites W2047399603 @default.
• W2594774920 cites W2049034575 @default.
• W2594774920 cites W2054891189 @default.
• W2594774920 cites W2084969272 @default.
• W2594774920 cites W2089134885 @default.
• W2594774920 cites W2099463509 @default.
• W2594774920 cites W2100455255 @default.
• W2594774920 cites W2112856904 @default.
• W2594774920 cites W2114520383 @default.
• W2594774920 cites W2125750727 @default.
• W2594774920 cites W2131852221 @default.
• W2594774920 cites W2132629607 @default.
• W2594774920 cites W2140092984 @default.
• W2594774920 cites W2144278357 @default.
• W2594774920 cites W2147645304 @default.
• W2594774920 cites W2152403136 @default.
• W2594774920 cites W2154606069 @default.
• W2594774920 cites W2165565773 @default.
• W2594774920 cites W2305931865 @default.
• W2594774920 cites W2342017170 @default.
• W2594774920 cites W2531349174 @default.
• W2594774920 doi "https://doi.org/10.1016/j.molimm.2016.12.015" @default.
• W2594774920 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28254726" @default.
• W2594774920 hasPublicationYear "2017" @default.
• W2594774920 type Work @default.
• W2594774920 sameAs 2594774920 @default.
• W2594774920 citedByCount "3" @default.
• W2594774920 countsByYear W25947749202017 @default.
• W2594774920 countsByYear W25947749202018 @default.
• W2594774920 countsByYear W25947749202022 @default.
• W2594774920 crossrefType "journal-article" @default.
• W2594774920 hasAuthorship W2594774920A5009690143 @default.
• W2594774920 hasAuthorship W2594774920A5011009080 @default.
• W2594774920 hasAuthorship W2594774920A5052276722 @default.
• W2594774920 hasAuthorship W2594774920A5053679121 @default.
• W2594774920 hasAuthorship W2594774920A5057157143 @default.
• W2594774920 hasAuthorship W2594774920A5090077495 @default.
• W2594774920 hasAuthorship W2594774920A5091384341 @default.
• W2594774920 hasBestOaLocation W25947749202 @default.
• W2594774920 hasConcept C104317684 @default.
• W2594774920 hasConcept C111684460 @default.
• W2594774920 hasConcept C12554922 @default.
• W2594774920 hasConcept C145148216 @default.
• W2594774920 hasConcept C178790620 @default.
• W2594774920 hasConcept C182629451 @default.
• W2594774920 hasConcept C185592680 @default.
• W2594774920 hasConcept C189446657 @default.
• W2594774920 hasConcept C203014093 @default.
• W2594774920 hasConcept C2182769 @default.
• W2594774920 hasConcept C2777339483 @default.
• W2594774920 hasConcept C32909587 @default.
• W2594774920 hasConcept C53345823 @default.
• W2594774920 hasConcept C55493867 @default.
• W2594774920 hasConcept C71240020 @default.
• W2594774920 hasConcept C8010536 @default.
• W2594774920 hasConcept C86803240 @default.
• W2594774920 hasConcept C8891405 @default.
• W2594774920 hasConceptScore W2594774920C104317684 @default.
• W2594774920 hasConceptScore W2594774920C111684460 @default.
• W2594774920 hasConceptScore W2594774920C12554922 @default.
• W2594774920 hasConceptScore W2594774920C145148216 @default.
• W2594774920 hasConceptScore W2594774920C178790620 @default.
• W2594774920 hasConceptScore W2594774920C182629451 @default.
• W2594774920 hasConceptScore W2594774920C185592680 @default.
• W2594774920 hasConceptScore W2594774920C189446657 @default.
• W2594774920 hasConceptScore W2594774920C203014093 @default.
• W2594774920 hasConceptScore W2594774920C2182769 @default.
• W2594774920 hasConceptScore W2594774920C2777339483 @default.
• W2594774920 hasConceptScore W2594774920C32909587 @default.
• W2594774920 hasConceptScore W2594774920C53345823 @default.
• W2594774920 hasConceptScore W2594774920C55493867 @default.

• next