FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2594828440> ?p ?o ?g. }

• W2594828440 endingPage "1911" @default.
• W2594828440 startingPage "1911" @default.
• W2594828440 abstract "Two-photon microscopy of cellular autofluorescence intensity and lifetime (optical metabolic imaging, or OMI) is a promising tool for preclinical drug development. OMI, which exploits the endogenous fluorescence from the metabolic coenzymes NAD(P)H and FAD, is sensitive to changes in cell metabolism produced by drug treatment. Previous studies have shown that drug response, genetic expression, cell-cell communication, and cell signaling in 3D culture match those of the original in vivo tumor, but not those of 2D culture. The goal of this study is to use OMI to quantify dynamic cell-level metabolic differences in drug response in 2D cell lines vs. 3D organoids generated from xenograft tumors of the same cell origin. BT474 cells and Herceptin-resistant BT474 (HR6) cells were tested. Cells were treated with vehicle control, Herceptin, XL147 (PI3K inhibitor), and the combination. The OMI index was used to quantify response, and is a linear combination of the redox ratio (intensity of NAD(P)H divided by FAD), mean NADH lifetime, and mean FAD lifetime. The results confirm that the OMI index resolves significant differences (p<0.05) in drug response for 2D vs. 3D cultures, specifically for BT474 cells 24 hours after Herceptin treatment, for HR6 cells 24 and 72 hours after combination treatment, and for HR6 cells 72 hours after XL147 treatment. Cell-level analysis of the OMI index also reveals differences in the number of cell sub-populations in 2D vs. 3D culture at 24, 48, and 72 hours post-treatment in control and treated groups. Finally, significant increases (p<0.05) in the mean lifetime of NADH and FAD were measured in 2D vs. 3D for both cell lines at 72 hours post-treatment in control and all treatment groups. These whole-population differences in the mean NADH and FAD lifetimes are supported by differences in the number of cell sub-populations in 2D vs. 3D. Overall, these studies confirm that OMI is sensitive to differences in drug response in 2D vs. 3D, and provides further information on dynamic changes in the relative abundance of metabolic cell sub-populations that contribute to this difference." @default.
• W2594828440 created "2017-03-16" @default.
• W2594828440 creator A5009288070 @default.
• W2594828440 creator A5084628898 @default.
• W2594828440 creator A5086337143 @default.
• W2594828440 date "2017-02-28" @default.
• W2594828440 modified "2023-10-17" @default.
• W2594828440 title "Autofluorescence imaging captures heterogeneous drug response differences between 2D and 3D breast cancer cultures" @default.
• W2594828440 cites W1564321524 @default.
• W2594828440 cites W1891860149 @default.
• W2594828440 cites W1892152977 @default.
• W2594828440 cites W1967293289 @default.
• W2594828440 cites W1971267920 @default.
• W2594828440 cites W1997400085 @default.
• W2594828440 cites W2033474014 @default.
• W2594828440 cites W2057280155 @default.
• W2594828440 cites W2074141198 @default.
• W2594828440 cites W2078091226 @default.
• W2594828440 cites W2083147890 @default.
• W2594828440 cites W2099225188 @default.
• W2594828440 cites W2109762821 @default.
• W2594828440 cites W2115759102 @default.
• W2594828440 cites W2119795324 @default.
• W2594828440 cites W2121388227 @default.
• W2594828440 cites W2129827453 @default.
• W2594828440 cites W2136445803 @default.
• W2594828440 cites W2140474512 @default.
• W2594828440 cites W2143036691 @default.
• W2594828440 cites W2166550586 @default.
• W2594828440 cites W2170349147 @default.
• W2594828440 cites W2192927701 @default.
• W2594828440 cites W2314492301 @default.
• W2594828440 cites W2515560644 @default.
• W2594828440 cites W2529113179 @default.
• W2594828440 cites W2572957424 @default.
• W2594828440 cites W4239989216 @default.
• W2594828440 doi "https://doi.org/10.1364/boe.8.001911" @default.
• W2594828440 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5480588" @default.
• W2594828440 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28663873" @default.
• W2594828440 hasPublicationYear "2017" @default.
• W2594828440 type Work @default.
• W2594828440 sameAs 2594828440 @default.
• W2594828440 citedByCount "26" @default.
• W2594828440 countsByYear W25948284402017 @default.
• W2594828440 countsByYear W25948284402018 @default.
• W2594828440 countsByYear W25948284402019 @default.
• W2594828440 countsByYear W25948284402020 @default.
• W2594828440 countsByYear W25948284402021 @default.
• W2594828440 countsByYear W25948284402022 @default.
• W2594828440 countsByYear W25948284402023 @default.
• W2594828440 crossrefType "journal-article" @default.
• W2594828440 hasAuthorship W2594828440A5009288070 @default.
• W2594828440 hasAuthorship W2594828440A5084628898 @default.
• W2594828440 hasAuthorship W2594828440A5086337143 @default.
• W2594828440 hasBestOaLocation W25948284401 @default.
• W2594828440 hasConcept C120665830 @default.
• W2594828440 hasConcept C121332964 @default.
• W2594828440 hasConcept C142724271 @default.
• W2594828440 hasConcept C1491633281 @default.
• W2594828440 hasConcept C150903083 @default.
• W2594828440 hasConcept C179687394 @default.
• W2594828440 hasConcept C181199279 @default.
• W2594828440 hasConcept C185592680 @default.
• W2594828440 hasConcept C207001950 @default.
• W2594828440 hasConcept C2780035454 @default.
• W2594828440 hasConcept C2994119904 @default.
• W2594828440 hasConcept C502942594 @default.
• W2594828440 hasConcept C54355233 @default.
• W2594828440 hasConcept C55493867 @default.
• W2594828440 hasConcept C62112901 @default.
• W2594828440 hasConcept C71924100 @default.
• W2594828440 hasConcept C75520062 @default.
• W2594828440 hasConcept C81885089 @default.
• W2594828440 hasConcept C86803240 @default.
• W2594828440 hasConcept C91881484 @default.
• W2594828440 hasConcept C98274493 @default.
• W2594828440 hasConceptScore W2594828440C120665830 @default.
• W2594828440 hasConceptScore W2594828440C121332964 @default.
• W2594828440 hasConceptScore W2594828440C142724271 @default.
• W2594828440 hasConceptScore W2594828440C1491633281 @default.
• W2594828440 hasConceptScore W2594828440C150903083 @default.
• W2594828440 hasConceptScore W2594828440C179687394 @default.
• W2594828440 hasConceptScore W2594828440C181199279 @default.
• W2594828440 hasConceptScore W2594828440C185592680 @default.
• W2594828440 hasConceptScore W2594828440C207001950 @default.
• W2594828440 hasConceptScore W2594828440C2780035454 @default.
• W2594828440 hasConceptScore W2594828440C2994119904 @default.
• W2594828440 hasConceptScore W2594828440C502942594 @default.
• W2594828440 hasConceptScore W2594828440C54355233 @default.
• W2594828440 hasConceptScore W2594828440C55493867 @default.
• W2594828440 hasConceptScore W2594828440C62112901 @default.
• W2594828440 hasConceptScore W2594828440C71924100 @default.
• W2594828440 hasConceptScore W2594828440C75520062 @default.
• W2594828440 hasConceptScore W2594828440C81885089 @default.
• W2594828440 hasConceptScore W2594828440C86803240 @default.
• W2594828440 hasConceptScore W2594828440C91881484 @default.
• W2594828440 hasConceptScore W2594828440C98274493 @default.
• W2594828440 hasFunder F4320306076 @default.

• next