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- W2594842011 abstract "Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap 1–7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual F o F 1 -ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object’s Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap." @default.
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- W2594842011 date "2017-02-27" @default.
- W2594842011 modified "2023-10-14" @default.
- W2594842011 title "Confining Brownian motion of single nanoparticles in an ABELtrap" @default.
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- W2594842011 doi "https://doi.org/10.1117/12.2250550" @default.
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