FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2594880291> ?p ?o ?g. }

• W2594880291 abstract "Background: An alarmingly large proportion of cancer-related deaths result from metastatic cancers. Development of quick, reliable and non-invasive or minimally invasive approaches to objectively assess the secondary tissues (potentially in vivo) will be instrumental in substantially reducing the cancer burden due to metastasis, which accounts for majority of cancer related mortality. In our study we report the utilization of Raman spectroscopy and chemometric techniques in identifying formation of pre-metastatic niche in lungs prior to observing morphological changes. Methods: Six-week-old female athymic nu/nu mice (NCI, MD) were implanted with 2x106 cells of human breast cancer cell lines - MDA-MB-231 (n=3), and MCF-7 (n=3) in their fourth right mammary fat pad orthotopically. And control mice (n=3) without tumor cell implantation were also employed. The primary tumor size was monitored and the mice were sacrificed within 8-12 weeks of cell implantation when the primary tumors volume grew to 500-600 mm3. Control mice were also sacrificed in this timeframe. The freshly excised lungs of the mice were cleaned in PBS and utilized for obtaining Raman spectra (830 nm, thermoelectrically cooled CCD). Each tissue was collected from multiple points. Principal component analysis (PCA) and Partial least squares discriminant analysis (PLS-DA) were employed as discriminating algorithm. Following spectral acquisition, the tissues were fixed in 10% formalin, embedded in paraffin, and then HE staining, and Masson9s trichrome staining for collagen. Collagen quantification of Masson9s trichrome stained slides was achieved using MATLAB (Mathworks, MA). The Institutional Animal Care and Use Committee at Johns Hopkins University School of Medicine approved the protocol of the study. Results: 900 Raman spectra each acquired from the lungs of the control mice and mice bearing MCF-7 and MDA-MB-231 tumor xenografts were assigned class labels - 9Control9, 9MCL9 and 9MDL9 respectively for further analysis. Select principal components from those obtained by subjecting all the chosen 900 spectra to PCA clearly evident that the differences in the Raman spectra belonging to tissues being primed by derivatives of different primary cells are quite pronounced. The average correct rates of PLS-DA prediction of 90.1%, 97.7% and 78.4% were obtained for the spectra belonging to the classes - Control, MCL and MDL respectively. The HE images are negative for any signs of cancerous lesions. Masson9s trichrome staining results show that the metastatic potential of the cell lines responsible for the primary tumor is positively correlated with the collagen density in the pre-metastatic niche, the MDL shows the highest collagen density (P Conclusion: The current study introduces Raman spectroscopy in conjunction with chemometric techniques as are liable and minimally invasive tool for diagnosis of metastatic cancers significantly early in the metastatic cascade, and also opens a new route for early targeting of cancer metastasis and its associated burden. Citation Format: Zheng C, Rizwan A, Paidi SK, Yu Z, Barman I, Glunde K. Early detection of development of a pre-metastatic niche in lungs in response to primary breast tumor using Raman spectroscopy [abstract]. In: Proceedings of the 2016 San Antonio Breast Cancer Symposium; 2016 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2017;77(4 Suppl):Abstract nr P6-01-18." @default.
• W2594880291 created "2017-03-16" @default.
• W2594880291 creator A5002196001 @default.
• W2594880291 creator A5017569819 @default.
• W2594880291 creator A5039728129 @default.
• W2594880291 creator A5046935079 @default.
• W2594880291 creator A5085631728 @default.
• W2594880291 creator A5087419436 @default.
• W2594880291 date "2017-02-14" @default.
• W2594880291 modified "2023-09-27" @default.
• W2594880291 title "Abstract P6-01-18: Early detection of development of a pre-metastatic niche in lungs in response to primary breast tumor using Raman spectroscopy" @default.
• W2594880291 doi "https://doi.org/10.1158/1538-7445.sabcs16-p6-01-18" @default.
• W2594880291 hasPublicationYear "2017" @default.
• W2594880291 type Work @default.
• W2594880291 sameAs 2594880291 @default.
• W2594880291 citedByCount "0" @default.
• W2594880291 crossrefType "proceedings-article" @default.
• W2594880291 hasAuthorship W2594880291A5002196001 @default.
• W2594880291 hasAuthorship W2594880291A5017569819 @default.
• W2594880291 hasAuthorship W2594880291A5039728129 @default.
• W2594880291 hasAuthorship W2594880291A5046935079 @default.
• W2594880291 hasAuthorship W2594880291A5085631728 @default.
• W2594880291 hasAuthorship W2594880291A5087419436 @default.
• W2594880291 hasConcept C121608353 @default.
• W2594880291 hasConcept C125473707 @default.
• W2594880291 hasConcept C126322002 @default.
• W2594880291 hasConcept C142724271 @default.
• W2594880291 hasConcept C150903083 @default.
• W2594880291 hasConcept C163031210 @default.
• W2594880291 hasConcept C204232928 @default.
• W2594880291 hasConcept C207001950 @default.
• W2594880291 hasConcept C2779013556 @default.
• W2594880291 hasConcept C2780283643 @default.
• W2594880291 hasConcept C530470458 @default.
• W2594880291 hasConcept C59469219 @default.
• W2594880291 hasConcept C71924100 @default.
• W2594880291 hasConcept C86803240 @default.
• W2594880291 hasConceptScore W2594880291C121608353 @default.
• W2594880291 hasConceptScore W2594880291C125473707 @default.
• W2594880291 hasConceptScore W2594880291C126322002 @default.
• W2594880291 hasConceptScore W2594880291C142724271 @default.
• W2594880291 hasConceptScore W2594880291C150903083 @default.
• W2594880291 hasConceptScore W2594880291C163031210 @default.
• W2594880291 hasConceptScore W2594880291C204232928 @default.
• W2594880291 hasConceptScore W2594880291C207001950 @default.
• W2594880291 hasConceptScore W2594880291C2779013556 @default.
• W2594880291 hasConceptScore W2594880291C2780283643 @default.
• W2594880291 hasConceptScore W2594880291C530470458 @default.
• W2594880291 hasConceptScore W2594880291C59469219 @default.
• W2594880291 hasConceptScore W2594880291C71924100 @default.
• W2594880291 hasConceptScore W2594880291C86803240 @default.
• W2594880291 hasLocation W25948802911 @default.
• W2594880291 hasOpenAccess W2594880291 @default.
• W2594880291 hasPrimaryLocation W25948802911 @default.
• W2594880291 hasRelatedWork W12531703 @default.
• W2594880291 hasRelatedWork W19745168 @default.
• W2594880291 hasRelatedWork W20193601 @default.
• W2594880291 hasRelatedWork W20799819 @default.
• W2594880291 hasRelatedWork W370019 @default.
• W2594880291 hasRelatedWork W3963582 @default.
• W2594880291 hasRelatedWork W6400718 @default.
• W2594880291 hasRelatedWork W7506178 @default.
• W2594880291 hasRelatedWork W7518501 @default.
• W2594880291 hasRelatedWork W7658715 @default.
• W2594880291 isParatext "false" @default.
• W2594880291 isRetracted "false" @default.
• W2594880291 magId "2594880291" @default.
• W2594880291 workType "article" @default.