FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2594929168> ?p ?o ?g. }

• W2594929168 endingPage "318" @default.
• W2594929168 startingPage "307" @default.
• W2594929168 abstract "The pro-inflammatory potency and causal relationship with asthma of inhaled endotoxins have underscored the importance of accurately assessing the endotoxin content of organic dusts. The Limulus amebocyte lysate (LAL) assay has emerged as the preferred assay, but its ability to measure endotoxin in intact bacteria and organic dusts with similar sensitivity as purified endotoxin is unknown. We used metabolically radiolabeled Neisseria meningitidis and both rough and smooth Escherichia coli to compare dose-dependent activation in the LAL with purified endotoxin from these bacteria and shed outer membrane (OM) blebs. Labeled [14C]-3-OH-fatty acids were used to quantify the endotoxin content of the samples. Purified meningococcal and E. coli endotoxins and OM blebs displayed similar specific activity in the LAL assay to the purified LPS standard. In contrast, intact bacteria exhibited fivefold lower specific activity in the LAL assay but showed similar MD-2-dependent potency as purified endotoxin in inducing acute airway inflammation in mice. Pre-treatment of intact bacteria and organic dusts with 0.1 M Tris-HCl/10 mM EDTA increased by fivefold the release of endotoxin. These findings demonstrate that house dust and other organic dusts should be extracted with Tris/EDTA to more accurately assess the endotoxin content and pro-inflammatory potential of these environmental samples." @default.
• W2594929168 created "2017-03-16" @default.
• W2594929168 creator A5012771682 @default.
• W2594929168 creator A5015680363 @default.
• W2594929168 creator A5017305734 @default.
• W2594929168 creator A5023095988 @default.
• W2594929168 creator A5025592571 @default.
• W2594929168 creator A5035513710 @default.
• W2594929168 creator A5041209504 @default.
• W2594929168 date "2017-02-26" @default.
• W2594929168 modified "2023-10-12" @default.
• W2594929168 title "Modification of sample processing for the<i>Limulus</i>amebocyte lysate assay enhances detection of inflammogenic endotoxin in intact bacteria and organic dust" @default.
• W2594929168 cites W1520507377 @default.
• W2594929168 cites W1550722396 @default.
• W2594929168 cites W1780456323 @default.
• W2594929168 cites W1782869644 @default.
• W2594929168 cites W1788623016 @default.
• W2594929168 cites W1837089874 @default.
• W2594929168 cites W1964346553 @default.
• W2594929168 cites W1968244810 @default.
• W2594929168 cites W1968299377 @default.
• W2594929168 cites W1974163512 @default.
• W2594929168 cites W1977545417 @default.
• W2594929168 cites W1980892269 @default.
• W2594929168 cites W1993540541 @default.
• W2594929168 cites W1997774756 @default.
• W2594929168 cites W2011097744 @default.
• W2594929168 cites W2024992082 @default.
• W2594929168 cites W2042914176 @default.
• W2594929168 cites W2044763085 @default.
• W2594929168 cites W2052202586 @default.
• W2594929168 cites W2057967701 @default.
• W2594929168 cites W2068365384 @default.
• W2594929168 cites W2069143195 @default.
• W2594929168 cites W2075368747 @default.
• W2594929168 cites W2082234253 @default.
• W2594929168 cites W2099936996 @default.
• W2594929168 cites W2103517048 @default.
• W2594929168 cites W2109659575 @default.
• W2594929168 cites W2110259476 @default.
• W2594929168 cites W2116287198 @default.
• W2594929168 cites W2120061656 @default.
• W2594929168 cites W2122116378 @default.
• W2594929168 cites W2129688641 @default.
• W2594929168 cites W2138882635 @default.
• W2594929168 cites W2141293464 @default.
• W2594929168 cites W2165308775 @default.
• W2594929168 cites W2165999456 @default.
• W2594929168 cites W2166538850 @default.
• W2594929168 cites W2171623243 @default.
• W2594929168 cites W2189291740 @default.
• W2594929168 cites W2255008104 @default.
• W2594929168 cites W2273396566 @default.
• W2594929168 cites W2416056314 @default.
• W2594929168 cites W2462845880 @default.
• W2594929168 cites W2484915396 @default.
• W2594929168 cites W32938029 @default.
• W2594929168 cites W4234317413 @default.
• W2594929168 cites W4254186801 @default.
• W2594929168 doi "https://doi.org/10.1177/1753425917694084" @default.
• W2594929168 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5814115" @default.
• W2594929168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28359219" @default.
• W2594929168 hasPublicationYear "2017" @default.
• W2594929168 type Work @default.
• W2594929168 sameAs 2594929168 @default.
• W2594929168 citedByCount "16" @default.
• W2594929168 countsByYear W25949291682017 @default.
• W2594929168 countsByYear W25949291682018 @default.
• W2594929168 countsByYear W25949291682019 @default.
• W2594929168 countsByYear W25949291682020 @default.
• W2594929168 countsByYear W25949291682021 @default.
• W2594929168 countsByYear W25949291682023 @default.
• W2594929168 crossrefType "journal-article" @default.
• W2594929168 hasAuthorship W2594929168A5012771682 @default.
• W2594929168 hasAuthorship W2594929168A5015680363 @default.
• W2594929168 hasAuthorship W2594929168A5017305734 @default.
• W2594929168 hasAuthorship W2594929168A5023095988 @default.
• W2594929168 hasAuthorship W2594929168A5025592571 @default.
• W2594929168 hasAuthorship W2594929168A5035513710 @default.
• W2594929168 hasAuthorship W2594929168A5041209504 @default.
• W2594929168 hasBestOaLocation W25949291681 @default.
• W2594929168 hasConcept C104317684 @default.
• W2594929168 hasConcept C151730666 @default.
• W2594929168 hasConcept C185592680 @default.
• W2594929168 hasConcept C202751555 @default.
• W2594929168 hasConcept C203014093 @default.
• W2594929168 hasConcept C2776040547 @default.
• W2594929168 hasConcept C2776487282 @default.
• W2594929168 hasConcept C2778458888 @default.
• W2594929168 hasConcept C2778754761 @default.
• W2594929168 hasConcept C2781014771 @default.
• W2594929168 hasConcept C43617362 @default.
• W2594929168 hasConcept C523546767 @default.
• W2594929168 hasConcept C54355233 @default.
• W2594929168 hasConcept C547475151 @default.
• W2594929168 hasConcept C55493867 @default.
• W2594929168 hasConcept C57409179 @default.
• W2594929168 hasConcept C57992300 @default.

• next