FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2595111200> ?p ?o ?g. }

• W2595111200 endingPage "1600696" @default.
• W2595111200 startingPage "1600696" @default.
• W2595111200 abstract "Yeast surface display, a well-established technology for protein analysis and engineering, involves expressing a protein of interest as a genetic fusion to either the N- or C-terminus of the yeast Aga2p mating protein. Historically, yeast-displayed protein variants are flanked by peptide epitope tags that enable flow cytometric measurement of construct expression using fluorescent primary or secondary antibodies. Here, we built upon this technology to develop a new yeast display strategy that comprises fusion of two different proteins to Aga2p, one to the N-terminus and one to the C-terminus. This approach allows an antibody fragment, ligand, or receptor to be directly coupled to expression of a fluorescent protein readout, eliminating the need for antibody-staining of epitope tags to quantify yeast protein expression levels. We show that this system simplifies quantification of protein-protein binding interactions measured on the yeast cell surface. Moreover, we show that this system facilitates co-expression of a bioconjugation enzyme and its corresponding peptide substrate on the same Aga2p construct, enabling enzyme expression and catalytic activity to be measured on the surface of yeast." @default.
• W2595111200 created "2017-03-23" @default.
• W2595111200 creator A5019187577 @default.
• W2595111200 creator A5032904785 @default.
• W2595111200 creator A5058204963 @default.
• W2595111200 creator A5082235599 @default.
• W2595111200 creator A5087304853 @default.
• W2595111200 date "2017-04-20" @default.
• W2595111200 modified "2023-10-10" @default.
• W2595111200 title "Dual display of proteins on the yeast cell surface simplifies quantification of binding interactions and enzymatic bioconjugation reactions" @default.
• W2595111200 cites W1485457998 @default.
• W2595111200 cites W1498780407 @default.
• W2595111200 cites W1584021532 @default.
• W2595111200 cites W1855382658 @default.
• W2595111200 cites W1954202239 @default.
• W2595111200 cites W1984760788 @default.
• W2595111200 cites W1985029586 @default.
• W2595111200 cites W1985796689 @default.
• W2595111200 cites W1986651564 @default.
• W2595111200 cites W1990789777 @default.
• W2595111200 cites W1999994305 @default.
• W2595111200 cites W2003134945 @default.
• W2595111200 cites W2017929146 @default.
• W2595111200 cites W2018295245 @default.
• W2595111200 cites W2024127494 @default.
• W2595111200 cites W2034160086 @default.
• W2595111200 cites W2041892339 @default.
• W2595111200 cites W2043627899 @default.
• W2595111200 cites W2050466522 @default.
• W2595111200 cites W2050940974 @default.
• W2595111200 cites W2055100713 @default.
• W2595111200 cites W2064995454 @default.
• W2595111200 cites W2065829656 @default.
• W2595111200 cites W2070360032 @default.
• W2595111200 cites W2071625217 @default.
• W2595111200 cites W2075601360 @default.
• W2595111200 cites W2081115093 @default.
• W2595111200 cites W2082432950 @default.
• W2595111200 cites W2091149944 @default.
• W2595111200 cites W2103336541 @default.
• W2595111200 cites W2130652548 @default.
• W2595111200 cites W2134210906 @default.
• W2595111200 cites W2136428466 @default.
• W2595111200 cites W2138006485 @default.
• W2595111200 cites W2149151801 @default.
• W2595111200 cites W2149603427 @default.
• W2595111200 cites W2153133904 @default.
• W2595111200 cites W2162481232 @default.
• W2595111200 cites W2197946614 @default.
• W2595111200 cites W2409911246 @default.
• W2595111200 cites W2427197815 @default.
• W2595111200 cites W2436853049 @default.
• W2595111200 cites W2504174252 @default.
• W2595111200 cites W2626473198 @default.
• W2595111200 cites W2749679400 @default.
• W2595111200 cites W4233808990 @default.
• W2595111200 cites W4239406033 @default.
• W2595111200 cites W4244541582 @default.
• W2595111200 cites W98408286 @default.
• W2595111200 doi "https://doi.org/10.1002/biot.201600696" @default.
• W2595111200 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5708543" @default.
• W2595111200 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28299901" @default.
• W2595111200 hasPublicationYear "2017" @default.
• W2595111200 type Work @default.
• W2595111200 sameAs 2595111200 @default.
• W2595111200 citedByCount "23" @default.
• W2595111200 countsByYear W25951112002017 @default.
• W2595111200 countsByYear W25951112002018 @default.
• W2595111200 countsByYear W25951112002019 @default.
• W2595111200 countsByYear W25951112002020 @default.
• W2595111200 countsByYear W25951112002021 @default.
• W2595111200 countsByYear W25951112002022 @default.
• W2595111200 countsByYear W25951112002023 @default.
• W2595111200 crossrefType "journal-article" @default.
• W2595111200 hasAuthorship W2595111200A5019187577 @default.
• W2595111200 hasAuthorship W2595111200A5032904785 @default.
• W2595111200 hasAuthorship W2595111200A5058204963 @default.
• W2595111200 hasAuthorship W2595111200A5082235599 @default.
• W2595111200 hasAuthorship W2595111200A5087304853 @default.
• W2595111200 hasBestOaLocation W25951112002 @default.
• W2595111200 hasConcept C104317684 @default.
• W2595111200 hasConcept C123894998 @default.
• W2595111200 hasConcept C147816474 @default.
• W2595111200 hasConcept C153911025 @default.
• W2595111200 hasConcept C159654299 @default.
• W2595111200 hasConcept C167625842 @default.
• W2595111200 hasConcept C181199279 @default.
• W2595111200 hasConcept C185592680 @default.
• W2595111200 hasConcept C186268636 @default.
• W2595111200 hasConcept C195616568 @default.
• W2595111200 hasConcept C23963754 @default.
• W2595111200 hasConcept C2776471321 @default.
• W2595111200 hasConcept C2777576037 @default.
• W2595111200 hasConcept C2779222958 @default.
• W2595111200 hasConcept C2779281246 @default.
• W2595111200 hasConcept C33161422 @default.
• W2595111200 hasConcept C40767141 @default.
• W2595111200 hasConcept C54355233 @default.

• next