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- W2595148785 abstract "The application of biotechnology in forest plantation industry is getting more popular over thelast decades and many researches have been conducted to develop marker-assisted selectionfor tree improvement programme in Malaysia. However, to date, marker assisted selection hasnot yet widely implemented in Sarawak and conventional breeding would take years to selectthe best characteristics in trees. Thus, this study was conducted to look into the effectivenessin using Expressed Sequenced Tag derived Simple Sequence Repeats (EST-SSRs) marker toestimate the distribution of genotypes and allele polymorphism of Acacia mangium superbulkpopulations in Sarawak; to determine the relationship or correlation between geneticvariation of these populations to environmental and physiological factors and to determine thegenetic differentiation of the Acacia mangium superbulk populations in comparison withother Acacia species. In this study, 93 fresh young leaf samples were collected from threedifferent areas, namely Similajau (Borneo Tree Seeds Seedlings Supply Sdn Bhd. (BTSSSB),Bintulu (DAIKEN) and Kota Samarahan (UNIMAS arboretum) and total genomic DNA ofthe samples were extracted using modified CTAB method. Thirteen EST-SSR markers werechosen to determine the genetic diversity of A. mangium superbulk and fragment analysis wasconducted by using ABI PRISM® 3100 Genetic Analyzer and Genemapper™ Version 4.0software (Applied Biosystems, USA). Statistical analysis performed using PowerMarkersoftware over 13 polymorphic loci revealed that the mean expected heterozygosity or He forthe three populations was 0.432 and polymorphic information content (PIC) value of 0.398,respectively. Borneo Tree Seeds Seedlings Supply Sdn. Bhd. population showed the highestlevel of genetic diversity (N = 40; He = 0.474; PIC = 0.432) compared to Daiken (N = 40; He= 0.458; PIC = 0.429) and UNIMAS Arboretum N = 13; He = 0.364; PIC = 0.333). Mean FSTxiiranged from 0.411 to 0.533 which indicates that the surplus of homozygotes generally occurwithin these populations. This might be due to inbreeding factor or partial selfing in A.mangium superbulk populations and Mean GST value of 0.038 further revealed that a lowgene differentiation between all populations (at 3.8%), which signifies that these populationsare genetically similar to each other. UPGMA dendogram was also constructed on the A.mangium superbulk populations and it revealed that the three populations belong to the samecluster, with BTSSSB and Daiken populations have the highest similarity coefficient (0.890).Future studies should look into thebreeding patterns in Acacia plus tree populations; the effectof silvicultural regimes in forest plantations to the genetic structure of Acacia species; and theeffect of interaction between temporal variations in outcrossing rates with temporal andspatial variations in seedling selection towards genetic structure of Acacia species" @default.
- W2595148785 created "2017-03-23" @default.
- W2595148785 creator A5011187304 @default.
- W2595148785 creator A5012719946 @default.
- W2595148785 date "2013-01-01" @default.
- W2595148785 modified "2023-09-27" @default.
- W2595148785 title "Genetic diversity of acacia mangium plus tree (superbulk) using est-ssr molecular marker" @default.
- W2595148785 hasPublicationYear "2013" @default.
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