FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2595301679> ?p ?o ?g. }

• W2595301679 endingPage "204" @default.
• W2595301679 startingPage "204" @default.
• W2595301679 abstract "Radio- and chemotherapy destroy reproductive germline cells in the gonads, causing infertility. Unlike men who have a clinically proven option, methods for preserving fertility in women and girls surviving cancer remain experimental. Remarkable advances in three-dimensional (3D) culture of mouse follicles in an alginate matrix leading to mature oocytes capable of fertilization with subsequent live offspring offer an opportunity to translate this technique to fertility preservation in women. The aims of this study were to validate whether a similar 3D scaffold permits the coordinated development of the nonhuman primate follicle in vitro, determine whether follicles collected from different stages of the menstrual cycle have similar developmental competence, and evaluate the role of gonadotropins, follicle stimulating hormone (FSH) alone or with luteinizing hormone (LH), to support folliculogenesis and steroidogenesis. Ovaries were obtained from rhesus monkeys during the early follicular (n=6 animals, 77 follicles) and midluteal phase (n=4 animals, 63 follicles) of normal menstrual cycles. Preantral follicles were mechanically isolated and encapsulated into 0.5% (w/v) alginate (FMC Biopolymers) beads. Encapsulated follicles were cultured individually in 250 μl αMEM containing 3 mg/ml BSA, 1mg/ml bovine fetuin, 5 ìg/ml insulin, 5 ìg/ml transferrin and 5 ng/ml selenium (Sigma) with either 0.5 mIU/ml recombinant human (r-h) FSH (NV Organon) alone or with 1.0 mIU r-hLH (Ares Serono) for 30 days at 37C in 5% CO2. Follicle survival and diameter were assessed weekly. Media collected on alternate days was analyzed for androstenedione (AD) and estradiol (E2). With FSH alone, 69% (35/51) of preantral follicles from the early follicular phase survived 30 days in culture, compared to 46% (12/26) from midluteal phase. Preantral follicles from the early follicular phase exposed to FSH + LH had a 33% (14/43) survival rate compared to 15% (3/20) from midluteal phase. Survival was greater (P<0.05) in preantral follicles from the early follicular phase regardless of gonadotropin treatment. Survival was also enhanced (P<0.05) with FSH alone relative to FSH + LH regardless of menstrual cycle stage. On day 1, follicle diameters were similar between the FSH (282 ± 16 μm) and FSH + LH groups (237 ± 12 μm). Follicle diameters were greater (P<0.05) on day 30 in the FSH group (735 ± 47 μm) relative to FSH + LH (528 ± 90 μm). Surviving follicles typically displayed an antral cavity by day 30. Production of AD and E2 was similar between gonadotropin groups throughout culture, and steadily increased to levels 4-fold higher (P<0.05) from day 7 to day 30. Thus, an alginate-based, 3D culture system supported survival, growth and steroid production of preantral follicles to the small antral stage in primates, particularly when preantral follicles originated from the early follicular phase. FSH alone improved follicle survival, promoted follicle growth and supported steroidogenesis. Whether the appropriate milieu for growth and differentiation of a preovulatory follicle and its enclosed oocyte in vitro requires LH remains to be determined. This novel technique provides, for the first time, a model to investigate follicle growth and maturation in primates, with the goal of producing meiotically and developmentally competent oocytes for fertility preservation in female cancer survivors. Supported by HD18185, RR00163, RR024347 Oncofertility Consortium." @default.
• W2595301679 created "2017-03-23" @default.
• W2595301679 creator A5016427677 @default.
• W2595301679 creator A5029655107 @default.
• W2595301679 creator A5030949220 @default.
• W2595301679 creator A5039555071 @default.
• W2595301679 creator A5041101727 @default.
• W2595301679 creator A5047403775 @default.
• W2595301679 creator A5073374356 @default.
• W2595301679 date "2008-05-01" @default.
• W2595301679 modified "2023-10-16" @default.
• W2595301679 title "An Alginate Matrix Supports the Three-dimensional Architecture of the Nonhuman Primate Follicle and Permits Coordinated Development of Preantral Follicles to Small Antral Follicles in Vitro." @default.
• W2595301679 doi "https://doi.org/10.1093/biolreprod/78.s1.204" @default.
• W2595301679 hasPublicationYear "2008" @default.
• W2595301679 type Work @default.
• W2595301679 sameAs 2595301679 @default.
• W2595301679 citedByCount "0" @default.
• W2595301679 crossrefType "journal-article" @default.
• W2595301679 hasAuthorship W2595301679A5016427677 @default.
• W2595301679 hasAuthorship W2595301679A5029655107 @default.
• W2595301679 hasAuthorship W2595301679A5030949220 @default.
• W2595301679 hasAuthorship W2595301679A5039555071 @default.
• W2595301679 hasAuthorship W2595301679A5041101727 @default.
• W2595301679 hasAuthorship W2595301679A5047403775 @default.
• W2595301679 hasAuthorship W2595301679A5073374356 @default.
• W2595301679 hasBestOaLocation W25953016791 @default.
• W2595301679 hasConcept C102570824 @default.
• W2595301679 hasConcept C126322002 @default.
• W2595301679 hasConcept C134018914 @default.
• W2595301679 hasConcept C142773365 @default.
• W2595301679 hasConcept C16685009 @default.
• W2595301679 hasConcept C179933525 @default.
• W2595301679 hasConcept C187785154 @default.
• W2595301679 hasConcept C196843134 @default.
• W2595301679 hasConcept C2777688143 @default.
• W2595301679 hasConcept C2778575703 @default.
• W2595301679 hasConcept C2778782382 @default.
• W2595301679 hasConcept C2779234561 @default.
• W2595301679 hasConcept C2779279165 @default.
• W2595301679 hasConcept C2780536345 @default.
• W2595301679 hasConcept C54355233 @default.
• W2595301679 hasConcept C5512455 @default.
• W2595301679 hasConcept C71315377 @default.
• W2595301679 hasConcept C71924100 @default.
• W2595301679 hasConcept C86803240 @default.
• W2595301679 hasConcept C95444343 @default.
• W2595301679 hasConceptScore W2595301679C102570824 @default.
• W2595301679 hasConceptScore W2595301679C126322002 @default.
• W2595301679 hasConceptScore W2595301679C134018914 @default.
• W2595301679 hasConceptScore W2595301679C142773365 @default.
• W2595301679 hasConceptScore W2595301679C16685009 @default.
• W2595301679 hasConceptScore W2595301679C179933525 @default.
• W2595301679 hasConceptScore W2595301679C187785154 @default.
• W2595301679 hasConceptScore W2595301679C196843134 @default.
• W2595301679 hasConceptScore W2595301679C2777688143 @default.
• W2595301679 hasConceptScore W2595301679C2778575703 @default.
• W2595301679 hasConceptScore W2595301679C2778782382 @default.
• W2595301679 hasConceptScore W2595301679C2779234561 @default.
• W2595301679 hasConceptScore W2595301679C2779279165 @default.
• W2595301679 hasConceptScore W2595301679C2780536345 @default.
• W2595301679 hasConceptScore W2595301679C54355233 @default.
• W2595301679 hasConceptScore W2595301679C5512455 @default.
• W2595301679 hasConceptScore W2595301679C71315377 @default.
• W2595301679 hasConceptScore W2595301679C71924100 @default.
• W2595301679 hasConceptScore W2595301679C86803240 @default.
• W2595301679 hasConceptScore W2595301679C95444343 @default.
• W2595301679 hasIssue "Suppl_1" @default.
• W2595301679 hasLocation W25953016791 @default.
• W2595301679 hasOpenAccess W2595301679 @default.
• W2595301679 hasPrimaryLocation W25953016791 @default.
• W2595301679 hasRelatedWork W2015670268 @default.
• W2595301679 hasRelatedWork W2017440399 @default.
• W2595301679 hasRelatedWork W2108394955 @default.
• W2595301679 hasRelatedWork W2120987058 @default.
• W2595301679 hasRelatedWork W2124506012 @default.
• W2595301679 hasRelatedWork W2124551859 @default.
• W2595301679 hasRelatedWork W2158095419 @default.
• W2595301679 hasRelatedWork W2166454430 @default.
• W2595301679 hasRelatedWork W2170775580 @default.
• W2595301679 hasRelatedWork W2261035098 @default.
• W2595301679 hasVolume "78" @default.
• W2595301679 isParatext "false" @default.
• W2595301679 isRetracted "false" @default.
• W2595301679 magId "2595301679" @default.
• W2595301679 workType "article" @default.