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- W2595480511 abstract "How the very first step in nucleosome assembly, deposition of histone H3-H4 as tetramers or dimers on DNA, is accomplished remains largely unclear. Here, we report that yeast chromatin assembly factor 1 (CAF1), a conserved histone chaperone complex that deposits H3-H4 during DNA replication, binds a single H3-H4 heterodimer in solution. We identify a new DNA-binding domain in the large Cac1 subunit of CAF1, which is required for high-affinity DNA binding by the CAF1 three-subunit complex, and which is distinct from the previously described C-terminal winged-helix domain. CAF1 binds preferentially to DNA molecules longer than 40 bp, and two CAF1-H3-H4 complexes concertedly associate with DNA molecules of this size, resulting in deposition of H3-H4 tetramers. While DNA binding is not essential for H3–H4 tetrasome deposition in vitro, it is required for efficient DNA synthesis-coupled nucleosome assembly. Mutant histones with impaired H3-H4 tetramerization interactions fail to release from CAF1, indicating that DNA deposition of H3-H4 tetramers by CAF1 requires a hierarchical cooperation between DNA binding, H3-H4 deposition and histone tetramerization." @default.
- W2595480511 created "2017-03-23" @default.
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- W2595480511 date "2017-03-18" @default.
- W2595480511 modified "2023-10-17" @default.
- W2595480511 title "Insights into the molecular architecture and histone H3-H4 deposition mechanism of yeast Chromatin assembly factor 1" @default.
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- W2595480511 cites W1854354834 @default.
- W2595480511 cites W1876643910 @default.
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- W2595480511 cites W1964737066 @default.
- W2595480511 cites W1965407215 @default.
- W2595480511 cites W1970304959 @default.
- W2595480511 cites W1973549360 @default.
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- W2595480511 cites W1988997227 @default.
- W2595480511 cites W1993774961 @default.
- W2595480511 cites W1994222671 @default.
- W2595480511 cites W1997519837 @default.
- W2595480511 cites W2001130038 @default.
- W2595480511 cites W2002191009 @default.
- W2595480511 cites W2008409661 @default.
- W2595480511 cites W2017470252 @default.
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- W2595480511 cites W2034104710 @default.
- W2595480511 cites W2042239071 @default.
- W2595480511 cites W2043707975 @default.
- W2595480511 cites W2044721366 @default.
- W2595480511 cites W2050091361 @default.
- W2595480511 cites W2052288218 @default.
- W2595480511 cites W2052540561 @default.
- W2595480511 cites W2054589275 @default.
- W2595480511 cites W2056734463 @default.
- W2595480511 cites W2060325238 @default.
- W2595480511 cites W2063771471 @default.
- W2595480511 cites W2065619780 @default.
- W2595480511 cites W2066011752 @default.
- W2595480511 cites W2069439721 @default.
- W2595480511 cites W2069681995 @default.
- W2595480511 cites W2072674961 @default.
- W2595480511 cites W2073449522 @default.
- W2595480511 cites W2077595353 @default.
- W2595480511 cites W2080578479 @default.
- W2595480511 cites W2081864633 @default.
- W2595480511 cites W2084396631 @default.
- W2595480511 cites W2086188832 @default.
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- W2595480511 cites W2145439094 @default.
- W2595480511 cites W2147990271 @default.
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- W2595480511 cites W2153653183 @default.
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- W2595480511 doi "https://doi.org/10.7554/elife.23474" @default.
- W2595480511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5404918" @default.
- W2595480511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28315525" @default.
- W2595480511 hasPublicationYear "2017" @default.
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