Matches in SemOpenAlex for { <https://semopenalex.org/work/W2595831794> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2595831794 endingPage "553" @default.
- W2595831794 startingPage "553" @default.
- W2595831794 abstract "Introduction Microbiological diagnosis of bone and joint infections (BJIs) currently relies on standard cultures which are time consuming and lack sensitivity. Various molecular approaches have been described and allowed improvement of BJI diagnosis. This study evaluated for the first time the performance of a DNA microarray-based assay (Prove-it™ Sepsis assay, PISA) for the rapid (<6 hours) detection and identification of 50 different species involved in BJI directly from clinical samples.Material and methods We retrospectively selected 130 bone and joint samples (67 synovial fluids and 63 bone biopsies) including 114 positive and 16 negative samples. The microbiological diagnosis had been previously established either by culture(C+, n=53) or by PCR16S and sequencing when culture was negative (C-/PCR+). The positive samples were selected to match the species targeted on the DNA microarray. DNA extraction was performed before proceeding to PISA amplification and hybridization on every selected sample.Results Among the 16 negative samples, one was detected positive with S. epidermidis by PISA, result that was secondarily confirmed using specific PCR. Among the 114 positive samples, 62.3% were positive using PISA with highly concordant identification compared to culture and PCR16S/sequencing results. Forty-three samples (37.7%) remained negative, illustrating a defect of sensitivity. However, PISA accurately detected methicillin resistance not only among the 16 C+/PISA+ Staphylococcus species (n=5) but also among the 28 C-/PCR16S+ Staphylococcus species (n=12) offering crucial rapid information to adapt the treatment of staphylococcal BJIs. Seven polymicrobial samples were also identified without extensive experiments.Discussion – Conclusion Even if the sensitivity deserves to be improved by optimizing DNA extraction and investigating on human DNA interference, these preliminary promising results highlight that this new and simple microarray method could be in the future an alternative to conventional PCR16S for the diagnosis of BJI." @default.
- W2595831794 created "2017-03-23" @default.
- W2595831794 creator A5008782369 @default.
- W2595831794 creator A5018967088 @default.
- W2595831794 creator A5039857818 @default.
- W2595831794 creator A5041670195 @default.
- W2595831794 creator A5053964411 @default.
- W2595831794 creator A5063696310 @default.
- W2595831794 creator A5066320819 @default.
- W2595831794 date "2012-09-01" @default.
- W2595831794 modified "2023-09-23" @default.
- W2595831794 title "EVALUATION OF A NEW MOLECULAR APPROACH USING DNA MICROARRAYS FOR THE DIAGNOSIS OF BONE AND JOINT INFECTION" @default.
- W2595831794 hasPublicationYear "2012" @default.
- W2595831794 type Work @default.
- W2595831794 sameAs 2595831794 @default.
- W2595831794 citedByCount "0" @default.
- W2595831794 crossrefType "journal-article" @default.
- W2595831794 hasAuthorship W2595831794A5008782369 @default.
- W2595831794 hasAuthorship W2595831794A5018967088 @default.
- W2595831794 hasAuthorship W2595831794A5039857818 @default.
- W2595831794 hasAuthorship W2595831794A5041670195 @default.
- W2595831794 hasAuthorship W2595831794A5053964411 @default.
- W2595831794 hasAuthorship W2595831794A5063696310 @default.
- W2595831794 hasAuthorship W2595831794A5066320819 @default.
- W2595831794 hasConcept C104317684 @default.
- W2595831794 hasConcept C141071460 @default.
- W2595831794 hasConcept C150194340 @default.
- W2595831794 hasConcept C153911025 @default.
- W2595831794 hasConcept C186836561 @default.
- W2595831794 hasConcept C2777949923 @default.
- W2595831794 hasConcept C2778120723 @default.
- W2595831794 hasConcept C2778336525 @default.
- W2595831794 hasConcept C2779288629 @default.
- W2595831794 hasConcept C2779489039 @default.
- W2595831794 hasConcept C3020326648 @default.
- W2595831794 hasConcept C49105822 @default.
- W2595831794 hasConcept C523546767 @default.
- W2595831794 hasConcept C54355233 @default.
- W2595831794 hasConcept C71924100 @default.
- W2595831794 hasConcept C78063203 @default.
- W2595831794 hasConcept C86803240 @default.
- W2595831794 hasConcept C89423630 @default.
- W2595831794 hasConcept C95371953 @default.
- W2595831794 hasConceptScore W2595831794C104317684 @default.
- W2595831794 hasConceptScore W2595831794C141071460 @default.
- W2595831794 hasConceptScore W2595831794C150194340 @default.
- W2595831794 hasConceptScore W2595831794C153911025 @default.
- W2595831794 hasConceptScore W2595831794C186836561 @default.
- W2595831794 hasConceptScore W2595831794C2777949923 @default.
- W2595831794 hasConceptScore W2595831794C2778120723 @default.
- W2595831794 hasConceptScore W2595831794C2778336525 @default.
- W2595831794 hasConceptScore W2595831794C2779288629 @default.
- W2595831794 hasConceptScore W2595831794C2779489039 @default.
- W2595831794 hasConceptScore W2595831794C3020326648 @default.
- W2595831794 hasConceptScore W2595831794C49105822 @default.
- W2595831794 hasConceptScore W2595831794C523546767 @default.
- W2595831794 hasConceptScore W2595831794C54355233 @default.
- W2595831794 hasConceptScore W2595831794C71924100 @default.
- W2595831794 hasConceptScore W2595831794C78063203 @default.
- W2595831794 hasConceptScore W2595831794C86803240 @default.
- W2595831794 hasConceptScore W2595831794C89423630 @default.
- W2595831794 hasConceptScore W2595831794C95371953 @default.
- W2595831794 hasLocation W25958317941 @default.
- W2595831794 hasOpenAccess W2595831794 @default.
- W2595831794 hasPrimaryLocation W25958317941 @default.
- W2595831794 hasRelatedWork W10958710 @default.
- W2595831794 hasRelatedWork W1679602198 @default.
- W2595831794 hasRelatedWork W1877676100 @default.
- W2595831794 hasRelatedWork W2005127454 @default.
- W2595831794 hasRelatedWork W2007396012 @default.
- W2595831794 hasRelatedWork W2022649880 @default.
- W2595831794 hasRelatedWork W2029290127 @default.
- W2595831794 hasRelatedWork W2139351680 @default.
- W2595831794 hasRelatedWork W2278012771 @default.
- W2595831794 hasRelatedWork W2369642242 @default.
- W2595831794 hasRelatedWork W2378619282 @default.
- W2595831794 hasRelatedWork W2592052532 @default.
- W2595831794 hasRelatedWork W2735460790 @default.
- W2595831794 hasRelatedWork W2745739184 @default.
- W2595831794 hasRelatedWork W2748444984 @default.
- W2595831794 hasRelatedWork W2985380793 @default.
- W2595831794 hasRelatedWork W3013036772 @default.
- W2595831794 hasRelatedWork W2124193330 @default.
- W2595831794 hasRelatedWork W2231555838 @default.
- W2595831794 hasRelatedWork W2887574824 @default.
- W2595831794 isParatext "false" @default.
- W2595831794 isRetracted "false" @default.
- W2595831794 magId "2595831794" @default.
- W2595831794 workType "article" @default.