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- W2597044478 abstract "Investigators identifying virulence genes at first did so by examining transposon mutants or individual gene mutations. Mutants of bacterial pathogens were then assessed in animals, whose symptoms mimicked human disease. Later, genome-wide screens were developed whereby genes and proteins that influence virulence could be identified, including via signature-tagged mutagenesis (STM), in vivo expression technology (IVET), and in vivo-induced antigen technology (IVIAT). Investigators then began to use RT-PCR to measure expression of individual genes, including within infected tissues. Microarray technology enables us to estimate global gene expression under defined culture conditions such as nitrogen limitation, oxygenation, and osmotic stress." @default.
- W2597044478 created "2017-03-23" @default.
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- W2597044478 date "2015-06-01" @default.
- W2597044478 modified "2023-09-27" @default.
- W2597044478 title "Redefining Virulence of Bacterial Pathogens" @default.
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- W2597044478 doi "https://doi.org/10.1128/microbe.10.239.1" @default.
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