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• W2597054145 abstract "Imbalance in the homeostasis of K+ ions has been reported to contribute to the pathogenesis of neurodegenerative diseases. 7-ketocholesterol (7KC), 24S-hydroxycholesterol (24S-OHC), and tetracosanoic acid (C24:0), often found at increased levels in patients with Alzheimer’s disease, Multiple Sclerosis and X-ALD, are able to trigger numerous nerve cell dysfunctions. We therefore studied the impact of 7KC, 24S-OHC, and C24:0 on 158N murine oligodendrocytes, and determined their impact on K+ homeostasis. The effects of 7KC, 24S-OHC and C24:0 on lipid membrane organization and membrane potential were examined with merocyanine 540 (MC540) and bis-(1,3-diethylthiobarbituric acid) trimethine oxonol (DiSBAC2(3)), respectively. The intracellular concentration of K+ ([K+]i) was measured by flame photometry and the ratiometric approach using the PBFI–AM fluorescence indicator. To determine the relationships between [K+]i and lipotoxicity, 158N cells were pre-treated with a universal Kv channels blocker, 4-aminopyridine (4-AP), without or with 7KC, 24S-OHC or C24:0. Cell adhesion, cell growth, mitochondrial depolarization, cytoplasmic membrane integrity, the presence of SubG1 and the morphological aspect of the nuclei were determined with various microscopy, flow cytometry and biochemistry methods. 7KC, 24S-OHC and C24:0 induced changes in lipid content and polarization of the cytoplasmic membrane. These events were associated with increased [K+]i. Blocking Kv channels with 4-AP exacerbated 7KC-, 24S-OHC- and C24:0-induced cell dysfunction. 4-AP exacerbated loss of cell adhesion and cell growth inhibition, amplified mitochondrial depolarization and cytoplasmic membrane damage, and increased the percentage of SubG1 cells. The positive correlation between [K+]i and cell death supports the potential involvement of K+ in 7KC-, 24S-OHC-, and C24:0-induced cytotoxicity." @default.
• W2597054145 created "2017-03-23" @default.
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• W2597054145 date "2017-10-01" @default.
• W2597054145 modified "2023-10-18" @default.
• W2597054145 title "Evidence of K+ homeostasis disruption in cellular dysfunction triggered by 7-ketocholesterol, 24S-hydroxycholesterol, and tetracosanoic acid (C24:0) in 158N murine oligodendrocytes" @default.
• W2597054145 cites W1480307543 @default.
• W2597054145 cites W1488851638 @default.
• W2597054145 cites W1493322929 @default.
• W2597054145 cites W1556974294 @default.
• W2597054145 cites W1620179564 @default.
• W2597054145 cites W1695534144 @default.
• W2597054145 cites W1798216359 @default.
• W2597054145 cites W1811472601 @default.
• W2597054145 cites W1879068300 @default.
• W2597054145 cites W1931744574 @default.
• W2597054145 cites W1962178044 @default.
• W2597054145 cites W1967917811 @default.
• W2597054145 cites W1969009199 @default.
• W2597054145 cites W1970189293 @default.
• W2597054145 cites W1971033954 @default.
• W2597054145 cites W1971571317 @default.
• W2597054145 cites W1972294486 @default.
• W2597054145 cites W1972896422 @default.
• W2597054145 cites W1973888479 @default.
• W2597054145 cites W1974666327 @default.
• W2597054145 cites W1981448470 @default.
• W2597054145 cites W1984911012 @default.
• W2597054145 cites W1989334958 @default.
• W2597054145 cites W1990632046 @default.
• W2597054145 cites W1992465104 @default.
• W2597054145 cites W1994766987 @default.
• W2597054145 cites W1998005444 @default.
• W2597054145 cites W2000948750 @default.
• W2597054145 cites W2014163855 @default.
• W2597054145 cites W2014212765 @default.
• W2597054145 cites W2015050725 @default.
• W2597054145 cites W2015185882 @default.
• W2597054145 cites W2017092830 @default.
• W2597054145 cites W2017274978 @default.
• W2597054145 cites W2023871339 @default.
• W2597054145 cites W2026011695 @default.
• W2597054145 cites W2027347547 @default.
• W2597054145 cites W2028509006 @default.
• W2597054145 cites W2030950431 @default.
• W2597054145 cites W2031163581 @default.
• W2597054145 cites W2033896868 @default.
• W2597054145 cites W2036433301 @default.
• W2597054145 cites W2038429688 @default.
• W2597054145 cites W2039429073 @default.
• W2597054145 cites W2039682492 @default.
• W2597054145 cites W2039974363 @default.
• W2597054145 cites W2046548513 @default.
• W2597054145 cites W2049678879 @default.
• W2597054145 cites W2049795698 @default.
• W2597054145 cites W2054295214 @default.
• W2597054145 cites W2056993355 @default.
• W2597054145 cites W2059203761 @default.
• W2597054145 cites W2059659588 @default.
• W2597054145 cites W2060722219 @default.
• W2597054145 cites W2071140636 @default.
• W2597054145 cites W2074581282 @default.
• W2597054145 cites W2075449056 @default.
• W2597054145 cites W2076404268 @default.
• W2597054145 cites W2076598376 @default.
• W2597054145 cites W2077819605 @default.
• W2597054145 cites W2078365361 @default.
• W2597054145 cites W2078815621 @default.
• W2597054145 cites W2080927052 @default.
• W2597054145 cites W2081051956 @default.
• W2597054145 cites W2083177766 @default.
• W2597054145 cites W2084192822 @default.
• W2597054145 cites W2084248110 @default.
• W2597054145 cites W2084331785 @default.
• W2597054145 cites W2085917831 @default.
• W2597054145 cites W2086011858 @default.
• W2597054145 cites W2089592802 @default.
• W2597054145 cites W2092382816 @default.
• W2597054145 cites W2093851813 @default.
• W2597054145 cites W2097057411 @default.
• W2597054145 cites W2110852753 @default.
• W2597054145 cites W2122096478 @default.
• W2597054145 cites W2123289792 @default.
• W2597054145 cites W2124956869 @default.
• W2597054145 cites W2125650861 @default.
• W2597054145 cites W2128775004 @default.
• W2597054145 cites W2129679875 @default.

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