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- W2597136004 abstract "In this study, vancomycin (Van) functionalized PEGylated-magnetic nanoparticles (Van-PMs) combined with polymerase chain reaction (PCR) showed great potential for highly efficient detection of L. monocytogenes at low infectious dose. Herein, Van-PMs played as universal molecular probes for targeting bacterial cells, while PEG was used to help Van close to D-alanyl-d-alanine (D-Ala-d-Ala) moieties on surface of L. monocytogenes. Under optimum conditions, the developed Van-PMs complexes exhibited extremely high capture efficiency (CE) in a 10 mL bacterium suspension with the value was higher than 90% and 83% in the phosphate-buffered saline (PBS) solution and lettuce samples, respectively. Meanwhile, Gram-negative bacteria slightly influenced CE even under concentrations as high as 106 CFU/mL. The assay of Van-PMs-PCR was applied to detect L. monocytogenes with detection limits of 30 CFU/mL and 30 CFU/g in PBS solution and lettuce samples, respectively. The whole experiment was completed within 4 h. This assay demonstrated the potential of this modified method in practical application involving the detection of L. monocytogenes or other Gram-positive pathogenic bacteria." @default.
- W2597136004 created "2017-03-23" @default.
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- W2597136004 date "2017-08-01" @default.
- W2597136004 modified "2023-10-06" @default.
- W2597136004 title "Vancomycin modified PEGylated-magnetic nanoparticles combined with PCR for efficient enrichment and detection of Listeria monocytogenes" @default.
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- W2597136004 doi "https://doi.org/10.1016/j.snb.2017.03.079" @default.
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